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Anonymous

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    #1102706 - 12/02/02 02:21 AM (14 years, 2 months ago)



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Offlinesocratesmind
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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1102726 - 12/02/02 02:28 AM (14 years, 2 months ago)

some people have used h2o2 solutions in there grains i've heard. chemically it is just different than water by the extra oxygen molecule :/ some people that have used it in growlogs are right here i know.
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Growlog&Number=609532&page=&view=&sb=&o=&fpart=1&vc=1


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Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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Invisiblezeta
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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1103129 - 12/02/02 05:02 AM (14 years, 2 months ago)

Straight peroxide? That's rediculous. Was it 3% or 6%?
I would dilute it to at least 1:10, more likely 1:20
When I make peroxidated agar I use about 1ml of 6% peroxide in 250ml of agar



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Offlinesocratesmind
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Re: How bad is H2O2 for mycelial tissue [Re: zeta]
    #1103137 - 12/02/02 05:09 AM (14 years, 2 months ago)

In reply to:

When I make peroxidated agar I use about 1ml of 6% peroxide in 250ml of agar




Well your ratio sounds like 1:16 and works well i guess so that must be a good range.


--------------------
Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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Anonymous

Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1103200 - 12/02/02 06:09 AM (14 years, 2 months ago)

i innoculated a mildly peroxidated (1ml @3% / 500ml of purified water) MYA mix w/ flammulina velutipes i got from anno. about a week later i innoculated MYA w/ no peroxide. the the non peroxidated plates are growing quicker as i would expect from the info provided on these boards, w/ no contams. out of the 6 peroxidated plates i started, 4 show slow but steady growth, the other 2 sit idle, i'm just hanging onto them for now... maybe the growing mycelia have been damaged in some way. i don't know. i hope to get a microscope soon... not sure if it will make it here before christmas. i'll probably preserve samples of each and see if there is a physical difference when i have one... maybe someone else knows the answer already. my work was done w/ spores...

u are using tissue samples so there's a big difference already. but straight up h2o2 is pretty kick ass from what i understand. if the oxidation damaged the outer layer of ur sample it may be all over.. i'll put this thread in my favorite, please keep us posted.

peace!



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Invisiblemycofile
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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1104112 - 12/02/02 03:39 PM (14 years, 2 months ago)

Peroxide can be used in agar, grains, and tissue sampling. For general use in a substrate (agar, grain etc.) you want a total peroxide content of .15% by weight.

In this case you're simply rinsing the tissue. 3% peroxide which is the most common concentration from a drug store in the US is fine for brief rinsings. It will bubble like crazy, have you never poured peroxide on an open wound before? That's what the stuff does, and is why it's used as an antiseptic.

You should be perfectly fine, assuming everything else was done properly.

BTW, I have extensive knowledge of peroxide if anybody has specific questions.


--------------------
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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1104575 - 12/02/02 06:23 PM (14 years, 2 months ago)

well i learned something today... thank u mycofile...


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InvisibleSixCee
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Re: How bad is H2O2 for mycelial tissue [Re: Anonymous]
    #1104986 - 12/02/02 08:49 PM (14 years, 2 months ago)

Yeah hydrogen peroxide is pretty harmless to mycelium and fruit bodies. I have even heard of a tek that uses h202 for its sterilization method.


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InvisibleTheEnemy
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Re: How bad is H2O2 for mycelial tissue [Re: SixCee]
    #1105231 - 12/02/02 10:13 PM (14 years, 2 months ago)

mmmmm, elements, percentages..... its good to see more chemistry on the boards.


--------------------
"Its not not a dollar bill" All statements for entertainment porposes only.


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Anonymous

Re: How bad is H2O2 for mycelial tissue [Re: SixCee]
    #1106408 - 12/03/02 07:26 AM (14 years, 2 months ago)

i've read the same thing... but i was under the impression even 3% solution direct expousure could damage mycelia. this is a good thing to know. the FV i'm culturing on peroxide is slow... but steady. another species spores just sit in the dish. no sign of contams, but no colinization either... i'm sitting tight for now. the teks that use peroxide seem pretty cool and the benefits from breakdown makes so much sense. i'm learning alot here...

one thing that i did do that wasn't mentioned... i don't want to derail this thread, but maybe someone can quickly comment on this fact and it's roll in my results so far...

i used 1.5 grams of yeast in a 500 ml batch.  :crazy: did i make bad?

thanks sixcee.  :cool:   


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Offlinezeronio
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Re: How bad is H2O2 for mycelial tissue [Re: Anonymous]
    #1106417 - 12/03/02 07:48 AM (14 years, 2 months ago)

Recomended is 1g per 500ml but you can use more without problems.


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Anonymous

Post deleted by Administrator [Re: Anonymous]
    #1106660 - 12/03/02 11:39 AM (14 years, 2 months ago)



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Offlinecomario2
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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1107023 - 12/03/02 02:24 PM (14 years, 2 months ago)

hi mycofile, i love peroxide myself, and i would like to take advantage of your offer to share on the topic. my specific questions are:

1. peroxide & grains. could explain how you do it? do you mix h2o2 with the grains before or after sterilization? please give all details;

2. peroxide & pan cyans. please note if there are any differences with respect to h2o2 & pcubensis.

thank you, i look forward to your precious knowledge :laugh: 


--------------------
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"crusaders against emotional poverty"


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Anonymous

Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1107296 - 12/03/02 03:54 PM (14 years, 2 months ago)

amazonians stalled on peroxide on me... any idea's mycofile? thank u in advance..


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Anonymous

Post deleted by Administrator [Re: ]
    #1115378 - 12/05/02 10:10 PM (14 years, 2 months ago)



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OfflineAzmodeus
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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1117172 - 12/06/02 02:35 PM (14 years, 2 months ago)

Yeah, i did some jars recently....and by day 5 was getting pretty nervous, but then low and behold!! patience is a virtue!


--------------------
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Lest we forget. "


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Anonymous

Re: How bad is H2O2 for mycelial tissue [Re: Azmodeus]
    #1117505 - 12/06/02 04:08 PM (14 years, 2 months ago)

h202 doesnt seem to hurt it to bad, ive mixed some with water before and used to to treat the cobweb mold, works most of the time if you catch it early


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InvisibleJoshua
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Re: How bad is H2O2 for mycelial tissue [Re: ]
    #1118312 - 12/06/02 08:41 PM (14 years, 2 months ago)

I use a 3% sol'n of peroxide in the same manner you did when I prepare cultures from wild specimens.

Joshua


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Offlinescifi5001
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Re: How bad is H2O2 for mycelial tissue [Re: mycofile]
    #1119299 - 12/07/02 04:29 AM (14 years, 2 months ago)

How strong peroxide solution would be needed for full desinfection?


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InvisibleHippie3
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Re: How bad is H2O2 for mycelial tissue [Re: scifi5001]
    #1119518 - 12/07/02 10:09 AM (14 years, 2 months ago)

the recommended dilution is 20:1,
water : 3% peroxide from the bottle.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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