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OfflineSmitington
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Registered: 08/10/09
Posts: 1,408
Loc: Mushroom Kingdom
Last seen: 11 years, 8 months
Contamination?
    #11020759 - 09/08/09 12:53 PM (15 years, 4 months ago)

My friend colonized 10 PES hawaiin jars strictly adhereing to the PF Tek.  His jars showed no signs of colonization until 3 weeks after inoculation, which seemed quite long.  Now, some of the spots where the spores where injected have nothing growing at all, and the others all have a white mycelium with dark blue on the inside.  It seems strange that all the jars would have the same kind of contamination if this blue coloring is contamination.  Perhaps the blue discoloration is a result of mycelium stress similiar to bruising, because it did take the spores soo long to do anything.  Any thoughts?  Just to clarify he did heat sterilize the needle between each jar, and no other contamination formed in the jars during the 3 weeks, so this seems to suggest that the jars were properly sterilized and that it would be unlikely for all the jars to have the same contamination at the injection sights.


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OfflineBuddhapaper
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Registered: 07/10/09
Posts: 30
Last seen: 14 years, 6 months
Re: Contamination? [Re: Smitington]
    #11020945 - 09/08/09 01:26 PM (15 years, 4 months ago)

What tempature did you keep the jars at during the incubation process, this could explain the delay for mycellum growth.I belive the blueish color is most likely a contamination. Also incubation tempatures range with different strains of fungi. Were the jars in a clean environment? Just because the jars were sterilized does not mean they are not susceptible to contamination during the incubation process.I like to keep my jars in a filterd enviroment during incubation to help elimate the possibilities of contamination.




Hawaiins were my first grow, such a wonderful strain:smile:

good luck

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Offlinettsk8r
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Registered: 01/23/07
Posts: 53
Last seen: 14 years, 1 month
Re: Contamination? [Re: Buddhapaper]
    #11023738 - 09/08/09 07:53 PM (15 years, 4 months ago)

Posting pictures would help.  However, it could be due to the lack of water since after all it is 3 weeks old which is causing them to bruise.. How many cc's of spores were injected into each cake?

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OfflineABC
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Registered: 11/16/08
Posts: 1,439
Last seen: 2 years, 8 months
Re: Contamination? [Re: ttsk8r]
    #11023966 - 09/08/09 08:36 PM (15 years, 4 months ago)

Probably a contamination. 3 weeks is too long and you also shouldn't be seeing any blue at this stage. Starting over is a good idea

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