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OfflinePeabody


Registered: 07/14/09
Posts: 123
Last seen: 13 years, 4 months
Bacteria.. myc disappearing after growth
    #11019619 - 09/08/09 09:02 AM (15 years, 4 months ago)

(Talking about WBS in quart jars here with P cubes.)

My first 20 or so quart jars went from inoculation to tubs without a hitch, very fast growth, no contams, easy peasy.

My last 20 jars have been hit or miss with bacteria. I'm trying to pin point it down, and I'm pretty sure it's my LC, but it's been tricky for me to figure out since I also switched birdseed brands and I believe one of the vendor spore syringes I ordered was dead to begin with (maybe because of the florida heat).

Anyway, the most common thing I see in my contaminated jars is slow initial growth, but definitely nice and white mycelial growth, after 2 days. This is from LC. Then, after 4 days, some of the myc starts disappearing. After 2 more days, there is no more visible myc in the jars. These jars look maybe slightly wet at the bottoms, not anything crazy. They definitely don't smell awful, but maybe sweeter and more like the birdseed than the successful jars.

In cases where I have PC'd 4 jars at a time and inoculated 1 straight from a vendor's live spore syringe, 2 with just LC originally from the same syringe, and 1 with both spore syringe and LC, the only one to make it is the one with just spores from the vendor syringe, so it must be the LC, right?

I'm just wondering why I do have growth at first that then disappears. I'm not absolutely sure it's bacteria, but that's what I'm thinking it is.

Also, if I have one jar that has bacterial contamination on the same shelf (but not touching or anything) as a good jar, how likely is it that the bacteria will spread? Lids are jar -> tyvek -> lid -> ring -> punched holes -> tyvek -> two layers paper tape.

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Offlinesolumvita
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Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 1 year, 7 months
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Re: Bacteria.. myc disappearing after growth [Re: Peabody]
    #11019975 - 09/08/09 10:33 AM (15 years, 4 months ago)

I think you said it yourself, your LC sounds like it is the problem.  As far as i know you should not get spread between closed jars.


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OfflineBrennus
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Registered: 05/31/08
Posts: 3,297
Loc: SE United States Flag
Last seen: 6 years, 10 months
Re: Bacteria.. myc disappearing after growth [Re: solumvita]
    #11026165 - 09/09/09 09:04 AM (15 years, 4 months ago)

That's the problem with sugar or honey-water based liquid cultures: It's hard to make sure your LC is clean.

Also, bacteria can't jump from jar to jar.

The new birdseed might also have a higher endospore count. Maybe you got sloppy with sterile technique. There's a lot of variables to chase down.

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