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Offlinetall dwarf
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Registered: 06/11/09
Posts: 199
Last seen: 15 years, 30 days
what would cause a brf jar to contam befores inoculation?
    #11016953 - 09/07/09 08:23 PM (15 years, 4 months ago)

Somebody sent me some halfpint brf jars and as soon as I removed them from the parcel I noticed they all had widespread green mold (either Trich or Penicillium).  The jars were never inoculated and had both a verm barrier AND a synthetic filter disc, and they still arrived contamed.
The sender assured me that his sterilization method is proper and he says he has some test jars from the same batch that are doing fine.  So he says the heat of transit must have caused this.  But how exactly could the heat of transit have caused widespread mold like this?  Wouldn't sterilization temps be hotter? 

So I guess the basic question is, what's the most likely reason a filtered brf jar would contam within a week of sterilization and before inoculation?

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Invisibleelemental420
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Registered: 08/10/09
Posts: 592
Loc: In da corn feilds.
Re: what would cause a brf jar to contam befores inoculation? [Re: tall dwarf]
    #11016978 - 09/07/09 08:27 PM (15 years, 4 months ago)

Does it really matter?

I mean either way you have to toss them, and why exactly dont you make your own?


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InvisibleDoc_T
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Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
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Re: what would cause a brf jar to contam befores inoculation? [Re: elemental420]
    #11017006 - 09/07/09 08:31 PM (15 years, 4 months ago)

A refund is in order. Unsterile jars were shipped.
Take refund money, buy RR's videos, then make your own jars.


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Invisiblefeelfunny
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Re: what would cause a brf jar to contam befores inoculation? [Re: Doc_T]
    #11017027 - 09/07/09 08:37 PM (15 years, 4 months ago)

good advice and way cheaper


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Offlinetall dwarf
Strange
Registered: 06/11/09
Posts: 199
Last seen: 15 years, 30 days
Re: what would cause a brf jar to contam befores inoculation? [Re: Doc_T]
    #11017055 - 09/07/09 08:41 PM (15 years, 4 months ago)

Quote:

elemental420 said:
Does it really matter?

I mean either way you have to toss them, and why exactly dont you make your own?




When things go wrong I like to know why.

I do make my own jars but I was traveling and wanted some jars ready for inoc when I returned home.

Quote:

Doc_T said:

A refund is in order. Unsterile jars were shipped.
Take refund money, buy RR's videos, then make your own jars.




This guy claims he kept some from the same batch and his are doing fine... Already own the DVDs.

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Offlinebusyskull
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Registered: 07/01/09
Posts: 62
Loc: Tincan Mountain
Last seen: 13 years, 4 months
Re: what would cause a brf jar to contam befores inoculation? [Re: tall dwarf]
    #11017175 - 09/07/09 09:06 PM (15 years, 4 months ago)

if you get some grain and start there you be doing alot more. you can even just lay the colinized grain out and fruit it. a refund:thumbup: definitly! like the other guy said DIY and you will be selp supportive, also, theres BRF agar cultures that make it a lot easier


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Invisibleelemental420
A student
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Registered: 08/10/09
Posts: 592
Loc: In da corn feilds.
Re: what would cause a brf jar to contam befores inoculation? [Re: tall dwarf]
    #11017242 - 09/07/09 09:22 PM (15 years, 4 months ago)

Quote:

Golden_Student said:
Quote:

elemental420 said:
Does it really matter?

I mean either way you have to toss them, and why exactly dont you make your own?




When things go wrong I like to know why.

I do make my own jars but I was traveling and wanted some jars ready for inoc when I returned home.


I hear ya but if you didnt prep the jars then there is no way you will know why they are no good.


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