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OfflineSevenHigh
SevenHigh
Male
Registered: 08/22/09
Posts: 7
Loc: AZ
Last seen: 14 years, 10 months
Loss of spore(syringe) viability from excessive heat??? Cannot achieve colonization of any sort...
    #11016406 - 09/07/09 06:49 PM (15 years, 4 months ago)

I recently purchased some 6 strains PC spore syringes from a provider  (Hawk) who happens to be a sponsor of this site.  I must say that Mr. Hawk has been more than accommodating and very helpful throughout this crisis and I am very appreciative of that. 

Here's the deal:

I am new to this hobby, and I thought I had some semblance of a brain in my head - so following the most basic PF Tek procedures didn't seem that intimidating.  But I am stumped.  I have put together LOTS of BRF cake jars, inoculated them in the cleanest of environments, carefully placed them in a dark area (75-80 deg) to incubate, and I have seen 0% colonization.  I even dabbled with WBS, followed accepted procedures and still....NOTHING.  I can't even grow any contaminants!!! Needless to say I am a little bummed, especially because Umphrey's is coming to town a in few days and I was planning a big night with the wife and friends. 

I live in Phoenix, which is the hottest place on earth.  I am wondering if the spores could have been rendered useless by sitting in some bin at the post office for hours at a time? 

I have made sure that my incubation temps are right on and I took special care to not pack the cakes in the jar too tight.  Is there any reason that any of you can come up with as to why I cannot seem to get any response from these spores?

I have used up 10 syringes with the same negative results every time.

Thanks so much for any insight!

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Invisibleavorg
Quixotic
Folding@home Statistics
Registered: 12/02/04
Posts: 1,348
Re: Loss of spore(syringe) viability from excessive heat??? Cannot achieve colonization of any sort... [Re: SevenHigh]
    #11016422 - 09/07/09 06:51 PM (15 years, 4 months ago)

heat can kill spores, but how long has it been?

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Invisibleelemental420
A student
Male User Gallery


Registered: 08/10/09
Posts: 592
Loc: In da corn feilds.
Re: Loss of spore(syringe) viability from excessive heat??? Cannot achieve colonization of any sort... [Re: SevenHigh]
    #11016424 - 09/07/09 06:51 PM (15 years, 4 months ago)

How many days now has it been since you knocked up the jars?


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