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SevenHigh
SevenHigh

Registered: 08/22/09
Posts: 7
Loc: AZ
Last seen: 14 years, 10 months
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Loss of spore(syringe) viability from excessive heat??? Cannot achieve colonization of any sort...
#11016406 - 09/07/09 06:49 PM (15 years, 4 months ago) |
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I recently purchased some 6 strains PC spore syringes from a provider (Hawk) who happens to be a sponsor of this site. I must say that Mr. Hawk has been more than accommodating and very helpful throughout this crisis and I am very appreciative of that.
Here's the deal:
I am new to this hobby, and I thought I had some semblance of a brain in my head - so following the most basic PF Tek procedures didn't seem that intimidating. But I am stumped. I have put together LOTS of BRF cake jars, inoculated them in the cleanest of environments, carefully placed them in a dark area (75-80 deg) to incubate, and I have seen 0% colonization. I even dabbled with WBS, followed accepted procedures and still....NOTHING. I can't even grow any contaminants!!! Needless to say I am a little bummed, especially because Umphrey's is coming to town a in few days and I was planning a big night with the wife and friends.
I live in Phoenix, which is the hottest place on earth. I am wondering if the spores could have been rendered useless by sitting in some bin at the post office for hours at a time?
I have made sure that my incubation temps are right on and I took special care to not pack the cakes in the jar too tight. Is there any reason that any of you can come up with as to why I cannot seem to get any response from these spores?
I have used up 10 syringes with the same negative results every time.
Thanks so much for any insight!
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avorg
Quixotic

Registered: 12/02/04
Posts: 1,348
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Re: Loss of spore(syringe) viability from excessive heat??? Cannot achieve colonization of any sort... [Re: SevenHigh]
#11016422 - 09/07/09 06:51 PM (15 years, 4 months ago) |
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heat can kill spores, but how long has it been?
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elemental420
A student



Registered: 08/10/09
Posts: 592
Loc: In da corn feilds.
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Re: Loss of spore(syringe) viability from excessive heat??? Cannot achieve colonization of any sort... [Re: SevenHigh]
#11016424 - 09/07/09 06:51 PM (15 years, 4 months ago) |
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How many days now has it been since you knocked up the jars?
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