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OfflineMrSpots
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Registered: 10/02/08
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whats wrong?
    #11006788 - 09/05/09 10:53 PM (15 years, 4 months ago)

So this is my first time growing mushrooms and i fallowed directions but something went wrong. After i sterilized the jar and let it cool over night, i inoculated them the next morning, and waited 2 weeks. and there is no sign of mycelium growth at all! the only thing that changed was where i inoculated there was yellow stain like piss. on the substrate.

what the yellow stain? and what went wrong? :sad::confused:


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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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InvisiblePolk_Audio3
Moon Cricket
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Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
Re: whats wrong? [Re: MrSpots]
    #11006833 - 09/05/09 11:00 PM (15 years, 4 months ago)

Did you flame sterilize you're needle after you knocked up each jar?
Give it a little more time.


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InvisibleUFOz8MyGoat
The Governator Sayz Go Chagaz
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Registered: 07/18/02
Posts: 1,924
Loc: USA
Re: whats wrong? [Re: Polk_Audio3]
    #11006856 - 09/05/09 11:04 PM (15 years, 4 months ago)

mycelium piss yellow maybe something went wrong ... could be mold or bacteria
if you post pics people can help

Edited by UFOz8MyGoat (09/05/09 11:05 PM)

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OfflineGlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
Re: whats wrong? [Re: Polk_Audio3]
    #11006865 - 09/05/09 11:05 PM (15 years, 4 months ago)

Did you remove the foil and tape?

Did you crack open the lids?

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OfflineMrSpots
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Re: whats wrong? [Re: GlassApex]
    #11006882 - 09/05/09 11:08 PM (15 years, 4 months ago)

The lids were rubber side up.
Yes i did flame sterilize it. but i only inoculated one jar.
i did remove the foil. i had no tape on it.
ill post pictures right now.


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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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InvisiblePolk_Audio3
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Re: whats wrong? [Re: MrSpots]
    #11006908 - 09/05/09 11:12 PM (15 years, 4 months ago)

Whats was you're ratio of substrate?


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OfflineMrSpots
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Re: whats wrong? [Re: MrSpots]
    #11006967 - 09/05/09 11:21 PM (15 years, 4 months ago)

1/2 pint jar:
140 ml vermiculite
40 ml brown rice flour



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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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OfflineGlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
Re: whats wrong? [Re: MrSpots]
    #11006982 - 09/05/09 11:25 PM (15 years, 4 months ago)

It should've been
140 ml vermiculite
70 ml brown rice flour

How much water?

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InvisibleUFOz8MyGoat
The Governator Sayz Go Chagaz
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Registered: 07/18/02
Posts: 1,924
Loc: USA
Re: whats wrong? [Re: MrSpots]
    #11006991 - 09/05/09 11:26 PM (15 years, 4 months ago)

you need the foil off so they can have gas exchange

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OfflineMrSpots
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Registered: 10/02/08
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Re: whats wrong? [Re: MrSpots]
    #11006999 - 09/05/09 11:28 PM (15 years, 4 months ago)

hmm would that affect the my mycelium growth?
and 20 ml of water.


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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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InvisiblePolk_Audio3
Moon Cricket
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Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
Re: whats wrong? [Re: MrSpots]
    #11007002 - 09/05/09 11:29 PM (15 years, 4 months ago)

Where did you get you're info on cultivating?

watch the pf tek www.mushroomvideos.com


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OfflineMrSpots
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Registered: 10/02/08
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Re: whats wrong? [Re: MrSpots]
    #11007007 - 09/05/09 11:30 PM (15 years, 4 months ago)

i got it from http://www.fungifun.org/English/Pftek
and ive watched that video plenty of times.


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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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InvisibleUFOz8MyGoat
The Governator Sayz Go Chagaz
Male


Registered: 07/18/02
Posts: 1,924
Loc: USA
Re: whats wrong? [Re: MrSpots]
    #11007017 - 09/05/09 11:32 PM (15 years, 4 months ago)

ya and it could give mold/bacteria the upper hand

an easy way to remember the mixture is
1 part water / 1 part BRF ./ 3 part verm
using a 1/2 pint this makes like 5/6 jars ... It's better to be on the dry side than the wet side because of contams

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OfflineGlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
Re: whats wrong? [Re: MrSpots]
    #11007031 - 09/05/09 11:35 PM (15 years, 4 months ago)

Yeah, your ratios were way off.

2:1:1

Verm:BRF:water

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OfflineMrSpots
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Registered: 10/02/08
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Last seen: 14 years, 6 months
Re: whats wrong? [Re: MrSpots]
    #11007046 - 09/05/09 11:37 PM (15 years, 4 months ago)

alright thanks guys. ill let you know what happens. ill take the foil off and give you an update.
also i forgot to tell you that the room temp is around 70 to 75 degrees fahrenheit.


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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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InvisiblePolk_Audio3
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Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
Re: whats wrong? [Re: GlassApex]
    #11007055 - 09/05/09 11:38 PM (15 years, 4 months ago)

Quote:

GlassApex said:
Yeah, your ratios were way off.

2:1:1

Verm:BRF:water





:thumbup:


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InvisibleUFOz8MyGoat
The Governator Sayz Go Chagaz
Male


Registered: 07/18/02
Posts: 1,924
Loc: USA
Re: whats wrong? [Re: GlassApex]
    #11007064 - 09/05/09 11:40 PM (15 years, 4 months ago)

Quote:

GlassApex said:
Yeah, your ratios were way off.

2:1:1

Verm:BRF:water



yes this is right

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OfflineMrSpots
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Registered: 10/02/08
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Re: whats wrong? [Re: UFOz8MyGoat]
    #11009305 - 09/06/09 01:06 PM (15 years, 4 months ago)

Good news guys! so last night i took the foil off and this morning there's a little spec of mycelium growing. :grin: i hope it goes good.


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:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2:Sometimes dreams really DOO come true!:mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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OfflineGlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
Re: whats wrong? [Re: MrSpots]
    #11011398 - 09/06/09 07:54 PM (15 years, 4 months ago)

Yeah, leave the foil off completely.

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