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OfflineNewpala21
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reuse syringes or no?
    #11009208 - 09/06/09 12:48 PM (15 years, 4 months ago)

was kinds wondering if its possible to reuse syringes that got left over spores in them. I mean if its possible why not right, but it seem like theyre all stuck to the plunger so how would i get them off and floating in water again without compromising the syringe and contaminating it?

Has anyone ever had success doin this and what steps should i take in trying to do so if possible


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OfflineHiddenIdentity
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Re: reuse syringes or no? [Re: Newpala21]
    #11009222 - 09/06/09 12:51 PM (15 years, 4 months ago)

I have re-used my syringes by boiling them, and making my own syringes out of them.  As far as trying to get the spores out that are still in there, if you boiled water, waited for it to cool, then suck up some water, that may work.

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Invisiblem00nshine
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Re: reuse syringes or no? [Re: Newpala21]
    #11009235 - 09/06/09 12:52 PM (15 years, 4 months ago)

"syringes that got left over spores in them"

Like, just in the syringe without water, or do you have some actual solution left? I say make an LC, they say you can make it with a drop of spore solution, not really experienced with that, though, sorry.

Edit: I do know you need 1 cc spore solution, 1 tablespoon (I believe) karo/honey, and 400ml of water....and a pressure cooker. I think you need to incubate the LC, too, but I'm not sure. You can make 40 syringes from this!!! :eek:

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InvisibleShea25
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Re: reuse syringes or no? [Re: Newpala21]
    #11009248 - 09/06/09 12:55 PM (15 years, 4 months ago)

Take a 1/2 pint jar with 1 hole in the lid taped up or self healing port, put water in it PC for 30 min let it cool down. In a glove box or sterile place flame the needle stick the needle in the hole suck up the sterile water. Now take a alcohol lamp or lighter heat up the needle till red keep the flame over the tip and suck in a few bubbles of air now shake like crazy the air in the syringe will help break up the spores

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OfflineNewpala21
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Re: reuse syringes or no? [Re: HiddenIdentity]
    #11009254 - 09/06/09 12:56 PM (15 years, 4 months ago)

i read somewhere along time ago that someone put jet dry(u know dishwasher shit)in the syringe to keep the spore from sticking but im not sure about that one there.  What u guys think?


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InvisibleShea25
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Re: reuse syringes or no? [Re: Newpala21]
    #11009263 - 09/06/09 12:58 PM (15 years, 4 months ago)

I herd that you can do that. I never had to I just make sure theres some sterile air in the syringe so I can shake em

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OfflineNewpala21
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Re: reuse syringes or no? [Re: m00nshine]
    #11009266 - 09/06/09 12:59 PM (15 years, 4 months ago)

Quote:

m00nshine said:
"syringes that got left over spores in them"

Like, just in the syringe without water, or do you have some actual solution left? I say make an LC, they say you can make it with a drop of spore solution, not really experienced with that, though, sorry.

Edit: I do know you need 1 cc spore solution, 1 tablespoon (I believe) karo/honey, and 400ml of water....and a pressure cooker. I think you need to incubate the LC, too, but I'm not sure. You can make 40 syringes from this!!! :eek:


no theyre empty just got spores in them still.

well theres a thought, 40 from that is a damn good deal and its alot cheaper


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Invisiblem00nshine
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Re: reuse syringes or no? [Re: Newpala21]
    #11009288 - 09/06/09 01:03 PM (15 years, 4 months ago)

Quote:

Newpala21 said:

no theyre empty just got spores in them still.well theres a thought, 40 from that is a damn good deal and its alot cheaper




I know, you can do it with less spores, too, I'm sure. Start researching, I know I will when the proper time arrives :gameover:


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Offlinekrygods
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Re: reuse syringes or no? [Re: m00nshine]
    #11009637 - 09/06/09 02:14 PM (15 years, 4 months ago)

boil water in a small pot and then suck the boiling water in the syringe and spray it out into the sink... do it about 4 or 5 times... then all you need to do it flame sterilize the needle... i use this method all the time with no problem at all... plus its a lot faster then throwing them in the PC or boiling them...

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OfflineNewpala21
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Re: reuse syringes or no? [Re: krygods]
    #11009688 - 09/06/09 02:22 PM (15 years, 4 months ago)

Quote:

krygods said:
boil water in a small pot and then suck the boiling water in the syringe and spray it out into the sink... do it about 4 or 5 times... then all you need to do it flame sterilize the needle... i use this method all the time with no problem at all... plus its a lot faster then throwing them in the PC or boiling them...



Did u even read the question/post at the top, ur answer dosent answer my question in the least bit. but thanx anyways for tryin, much appreciated


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Invisiblemister
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Re: reuse syringes or no? [Re: Newpala21]
    #11009729 - 09/06/09 02:31 PM (15 years, 4 months ago)

I boil distilled water and fill syringes, place in a quart jar with no lid. Place these in my P.C. with my grain jars. Then pc the whole 90 min at 15 psi. while these are cooling down, I also p.c. a half piint of distilled water with an injection port in the lid as well. When all is cool, I have jars, and syringes ready to go. All work is done in front of a flow hood as well.


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:mushroomgrow::mushroomgrow::mushroomgrow::mushroomgrow:AMU Q & A thread:mushroomgrow::mushroomgrow::mushroomgrow::mushroomgrow:

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InvisibleAuxin
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Re: reuse syringes or no? [Re: Newpala21]
    #11010981 - 09/06/09 06:26 PM (15 years, 4 months ago)

Quote:

Newpala21 said:
i read somewhere along time ago that someone put jet dry(u know dishwasher shit)in the syringe to keep the spore from sticking but im not sure about that one there.  What u guys think?



RR has said that Jet Dry will work to keep spores suspended better but he generally seems to advocate sex lube more (perhaps cause its buffered better so it wont burn your fun bits and thus is gentler to spores?)
Quote:

RogerRabbit said:
I think you're confusing dish soap with jet dry, used to avoid spots on glasses when washed in a dishwasher.  It's sometimes added to spore syringes to help prevent the spores from sticking.  A better choice is sex lube such as astroglide. Use one or two drops per 100ml prior to sterilization.
RR



[1]


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The Nook

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Offlinechillywilly419
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Re: reuse syringes or no? [Re: Newpala21]
    #11011183 - 09/06/09 07:05 PM (15 years, 4 months ago)

prepare a jar of sterile water-won't need much, a half pint jar
will do. in a glovebox, suck the sterile water into your syringe
and shake, that's it...test it out on a brf jar or two and store it.
if the jars come out clean, you can 'noc' up a few more jars or make
a liquid culture with it.
i would imagine all of this would have a much higher chance of success
if it was done as quickly as possible after the syringes initial use.

Search liquid culture, and syringe-making teks


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OfflineNewpala21
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Re: reuse syringes or no? [Re: chillywilly419]
    #11014432 - 09/07/09 12:21 PM (15 years, 4 months ago)

ok thanx chilly and all u other guys who gave me some feed back. i just scraped some spores into a hlf pint jar and im gonna boil some water and let it cool,suck it up and squirt it in the jar and suck it up agn. does that sound ok to u guys or ia that to risky, by the way the spores are just sitting in the empty jar think that will b ok till i move on with the procedure..
 
  Again, sorry i didnt mean to quote u, got a little trigger happy and hit the wrong one


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OfflineNewpala21
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Re: reuse syringes or no? [Re: Newpala21]
    #11017179 - 09/07/09 09:07 PM (15 years, 4 months ago)

Well i did it. i took a half pint that was already cleaned and i rubbed it down wit rubbing alcohol, the lid as well, and scrapped a whole spore print in to it. Then i dropped a marble that was boiled for 20min in with the spores and a couple drops of some sex lube and sealed it. The lid has 4 holes in it(12,3,6,and 9 o-clock) that are taped with two layers of micro tape.

    Now i got some water to a rolling boil and sucked it up to about 9cc's. I then injected through the tape and squirted it out real hard to brake up the spores, when i was removing the needle i quickly covered the hole with another piece of micro tape and dipped my finger into some rubbing alcohol and dabbed the tape with it so it got a drop or 2 on it. This was repeated 3 more time( once for each hole) and the needle was heated till it was red hot before i injected to each hole . Then gave it a good swirl so the marble would brake the rest of the spores up even better.

  When all this was said and done i put it on a a/c vent so it would cool quickly. Now my only question is, should i now put it in the pc for about 30mins or u guys think the steps i took were ok and everything should b OK. I would have used a self healing port but i have yet to get or make one.
 
    So ppl lemme know what u guys think on how i did this and if i should pc it for the hell of it just to b sure. Ima keep my fingers  crossed and hope for the best, will b innocing 2marrow so we will c what happens in about a week and how its goin...

        Its funny how certain parts of this post sound really dirty, like its spore porn :rofl2:

Edited by Newpala21 (09/07/09 09:10 PM)

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Offlinelsatrap
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Re: reuse syringes or no? [Re: Newpala21]
    #11017218 - 09/07/09 09:16 PM (15 years, 4 months ago)

I think your going to be in good shape..


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At this moment you should be with us, feeling like we do.


               

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Invisibleratdog
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Re: reuse syringes or no? [Re: Newpala21]
    #11017222 - 09/07/09 09:16 PM (15 years, 4 months ago)

W T F ?


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: ratdog]
    #11017281 - 09/07/09 09:29 PM (15 years, 4 months ago)

Quote:

ratdog said:
W T F ?



why u wtf-ing me what i do to deserve that


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Offlinefungusfeller
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Re: reuse syringes or no? [Re: Newpala21]
    #11017366 - 09/07/09 09:48 PM (15 years, 4 months ago)

i must have missed something, you put boiling water on your spores? and now you want to pc it?


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Invisibleratdog
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Re: reuse syringes or no? [Re: fungusfeller]
    #11017399 - 09/07/09 09:53 PM (15 years, 4 months ago)

Quote:

fungusfeller said:
i must have missed something, you put boiling water on your spores? and now you want to pc it?





WTF

what he said

sorry man don't do that often


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: ratdog]
    #11017506 - 09/07/09 10:11 PM (15 years, 4 months ago)

So im gonna assume that i shouldnt have put boiling water on them. I did do some reading before hand but i didnt see anything saying no to boiling water. Well if thats the case then i guess ill have to start over again,O-well,i got more prints anyways. plus it was my first attempt so i guess i can slide on trial an error with this one.. So besides that were the rest of my steps ok, if i did this again and let the water cool this time (dumb ass i'am) think it would work.


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OfflineNewpala21
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Re: reuse syringes or no? [Re: fungusfeller]
    #11017557 - 09/07/09 10:19 PM (15 years, 4 months ago)

Quote:

fungusfeller said:
i must have missed something, you put boiling water on your spores? and now you want to pc it?



yes i did  :shrug: and i know i shouldnt have done that now. the pc part i wasnt gonna do i was just askin if i should. first attempt here, gimmie some slack, swear i wont abuse spores like that again I PROMISE to all of u, my bad.


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Offlinefungusfeller
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Re: reuse syringes or no? [Re: Newpala21]
    #11017578 - 09/07/09 10:23 PM (15 years, 4 months ago)

just chalk it up as a lesson learned:shrug:


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TIMMY PUKESPLASH

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Invisibleratdog
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Re: reuse syringes or no? [Re: Newpala21]
    #11017650 - 09/07/09 10:35 PM (15 years, 4 months ago)

Quote:

Newpala21 said:
Quote:

fungusfeller said:
i must have missed something, you put boiling water on your spores? and now you want to pc it?



yes i did  :shrug: and i know i shouldn't have done that now. the pc part i wasn't gonna do i was just asking if i should. first attempt here, gimme some slack, swear i wont abuse spores like that again I PROMISE to all of u, my bad.





i don't think oyster spores can Handel that hot a temp.

but if you try again then i bet it might work

so what ever happens to the old packed plunger are you trying to pack more onto it now.

did you try the jet dry or my idea would have been to do a pint lc and back wash the hell out of it 10-15 times into the lc and wait.
if that worked then i would split the lc to pints same way but with clean tools. i got a master multi spore lc i have been using to knock qt lc's out the park.

waiting for the perfect oyster to clone then i will retire the multi Sp lc and start a master clone lc

i don't usualy wtf people but you changed the ball game in the 9th inning.

sorry


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: fungusfeller]
    #11017651 - 09/07/09 10:35 PM (15 years, 4 months ago)

Just as knowledge to myself, did the boiling water for say kill the spores.


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Invisibleratdog
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Re: reuse syringes or no? [Re: Newpala21]
    #11017671 - 09/07/09 10:37 PM (15 years, 4 months ago)

holly WTF!


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: ratdog]
    #11017719 - 09/07/09 10:42 PM (15 years, 4 months ago)

Quote:

ratdog said:
Quote:

Newpala21 said:
Quote:

fungusfeller said:
i must have missed something, you put boiling water on your spores? and now you want to pc it?



yes i did  :shrug: and i know i shouldn't have done that now. the pc part i wasn't gonna do i was just asking if i should. first attempt here, gimme some slack, swear i wont abuse spores like that again I PROMISE to all of u, my bad.





i don't think oyster spores can Handel that hot a temp.

but if you try again then i bet it might work

so what ever happens to the old packed plunger are you trying to pack more onto it now.

did you try the jet dry or my idea would have been to do a pint lc and back wash the hell out of it 10-15 times into the lc and wait.
if that worked then i would split the lc to pints same way but with clean tools. i got a master multi spore lc i have been using to knock qt lc's out the park.

waiting for the perfect oyster to clone then i will retire the multi Sp lc and start a master clone lc

i don't usualy wtf people but you changed the ball game in the 9th inning.

sorry



Well now i see i deserved the WTF so its all good, no harm no foul. Im not sure i understand what ur askin me about the plungers though, am i tryin to pack more what onto what? got me confused with that one, sorry


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Invisibleratdog
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Re: reuse syringes or no? [Re: Newpala21]
    #11017761 - 09/07/09 10:47 PM (15 years, 4 months ago)

Quote:

Newpala21 said:
was kinds wondering if its possible to reuse syringes that got left over spores in them. I mean if its possible why not right, but it seem like theyre all stuck to the plunger so how would i get them off and floating in water again without compromising the syringe and contaminating it?

Has anyone ever had success doin this and what steps should i take in trying to do so if possible





W T Holly F man lay off the glue eat something helthy and drink some water. then go get checked out.


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: Newpala21]
    #11017772 - 09/07/09 10:49 PM (15 years, 4 months ago)

Just so im clear, when i redo this(with water that has cooled down of course :wink:) dont pressure cook cause the same shit will happen again, correct,again just so im clear on this.


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Invisibleratdog
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Re: reuse syringes or no? [Re: ratdog]
    #11017817 - 09/07/09 10:58 PM (15 years, 4 months ago)

LMAO!!!!:eek:


post insert

yes but if the plunger has spores then rinse it out with boiling water
a few times so that the old stuff does not contam the new stuff. i would not trust the tool you got there no telling what the last guy did with the spores he might have shot up with it. were did you find this tool.

i do the boiling water thing too but i put a full draw of alcohol in the works also followed by a boiling water draw at end of cleaning my tools.

lc man try it the one with lme .... light malt extract and corn sugar

best thing man


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: ratdog]
    #11017931 - 09/07/09 11:23 PM (15 years, 4 months ago)

Hey ratdog  i was watchin ur video u got on you tube and im curious as to why u sterilized ur h-poo. It will more than likely get contaminated before ur first flush or right after the first..  I sterilized mine the first time i used it and the shit went bad( pun intended).


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Invisibleratdog
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Re: reuse syringes or no? [Re: Newpala21]
    #11017981 - 09/07/09 11:32 PM (15 years, 4 months ago)

Quote:

Newpala21 said:
Hey ratdog  i was watching Ur video u got on you tube and im curious as to why u sterilized ur h-poo. It will more than likely get contaminated before ur first flush or right after the first..  I sterilized mine the first time i used it and the shit went bad( pun intended).






from the video

also from the same ice cream pies





no contam yet but you might be right............but i also did not pc but for 12 min so if you think 12 min at 15 psi ant the same as pasture thin you might be right i do not know but we will see

BTW i have had 2 harvests from them cakes and will do it again in 2 days.......best stuff with eggs man


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
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Re: reuse syringes or no? [Re: ratdog]
    #11020819 - 09/08/09 01:02 PM (15 years, 4 months ago)

well if the shit is workin for ya then keep it goin. when i first used it i pc'd it till the rocker started rockin and then i turned it off, after so i went and posted what i was doin and like everyone and their mom said that i shouldnt have dont that i will contam, and sure enough it did right before the first harvest and my other bin right after the 2nd. Hey but if its workin dont lemme stop ya from doin what ur doin, best of luck to ya


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OfflineNewpala21
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Re: reuse syringes or no? [Re: Newpala21]
    #11020865 - 09/08/09 01:12 PM (15 years, 4 months ago)

Ok , so i went and pc'd everything and started over with the makin of my syringes, got the spores chillin in the jar wating for the water to cool(how cool should it b) in the pc'er and im gonna go for it so hopefully all will go well..

  I got some brf,wbs,and rye jars all ready to go so im gonna noc one of each up and c how it goes. Also i mixed some GT and EQ spores together to see what i get and then hopefully if i get one nice boomer i will attempt to mk some LC, so say a prayer for me all and ill keep u guys updated on that one.


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Offlinefungusfeller
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Re: reuse syringes or no? [Re: Newpala21]
    #11020901 - 09/08/09 01:18 PM (15 years, 4 months ago)

good luck
:popcorn:


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TIMMY PUKESPLASH

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Invisibleratdog
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Re: reuse syringes or no? [Re: fungusfeller]
    #11023978 - 09/08/09 08:39 PM (15 years, 4 months ago)

Quote:

fungusfeller said:
good luck
:popcorn:





shroom gods may they shine


--------------------
some people just don't get it:spank:
http://www.shroomery.org/forums/showflat.php/Number/11241796
so here is a video or two or three for you guys:rolleyes:

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OfflineNewpala21
1st time farmer
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Registered: 10/16/08
Posts: 371
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Re: reuse syringes or no? [Re: ratdog]
    #11024643 - 09/08/09 10:24 PM (15 years, 4 months ago)

AAAAAAAA    :sunny:
                        :mushroom2:


  :ooo: its alive, ITS ALIVE hahahahaha  :crazy2:


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OfflineNewpala21
1st time farmer
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Registered: 10/16/08
Posts: 371
Last seen: 15 years, 3 months
Re: reuse syringes or no? [Re: Newpala21]
    #11027583 - 09/09/09 01:57 PM (15 years, 4 months ago)

OK, so i went and got some agar and i got my honey but im not sure if this kind is fine. I have seen ppl using BEEMADE brand honey but the kind i got is the WILD HARVEST brand and its organic. probally a dumb question but i wanna be sure that this kind is ok to use before i open it and get stuck with honey i dont need. So will this kind be safe to use or should i get the BEEMADE brand instead?


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