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MrSpots
Stranger



Registered: 10/02/08
Posts: 10
Last seen: 14 years, 6 months
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whats wrong?
#11006788 - 09/05/09 10:53 PM (15 years, 4 months ago) |
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So this is my first time growing mushrooms and i fallowed directions but something went wrong. After i sterilized the jar and let it cool over night, i inoculated them the next morning, and waited 2 weeks. and there is no sign of mycelium growth at all! the only thing that changed was where i inoculated there was yellow stain like piss. on the substrate.
what the yellow stain? and what went wrong? 
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                       Sometimes dreams really DOO come true!                     
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Polk_Audio3
Moon Cricket



Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
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Re: whats wrong? [Re: MrSpots]
#11006833 - 09/05/09 11:00 PM (15 years, 4 months ago) |
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Did you flame sterilize you're needle after you knocked up each jar? Give it a little more time.
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UFOz8MyGoat
The Governator Sayz Go Chagaz



Registered: 07/18/02
Posts: 1,924
Loc: USA
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mycelium piss yellow maybe something went wrong ... could be mold or bacteria if you post pics people can help
Edited by UFOz8MyGoat (09/05/09 11:05 PM)
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GlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
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Did you remove the foil and tape?
Did you crack open the lids?
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MrSpots
Stranger



Registered: 10/02/08
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Last seen: 14 years, 6 months
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The lids were rubber side up. Yes i did flame sterilize it. but i only inoculated one jar. i did remove the foil. i had no tape on it. ill post pictures right now.
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                       Sometimes dreams really DOO come true!                     
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Polk_Audio3
Moon Cricket



Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
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Re: whats wrong? [Re: MrSpots]
#11006908 - 09/05/09 11:12 PM (15 years, 4 months ago) |
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Whats was you're ratio of substrate?
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MrSpots
Stranger



Registered: 10/02/08
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Last seen: 14 years, 6 months
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Re: whats wrong? [Re: MrSpots]
#11006967 - 09/05/09 11:21 PM (15 years, 4 months ago) |
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1/2 pint jar: 140 ml vermiculite 40 ml brown rice flour
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                       Sometimes dreams really DOO come true!                     
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GlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
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Re: whats wrong? [Re: MrSpots]
#11006982 - 09/05/09 11:25 PM (15 years, 4 months ago) |
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It should've been 140 ml vermiculite 70 ml brown rice flour
How much water?
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UFOz8MyGoat
The Governator Sayz Go Chagaz



Registered: 07/18/02
Posts: 1,924
Loc: USA
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Re: whats wrong? [Re: MrSpots]
#11006991 - 09/05/09 11:26 PM (15 years, 4 months ago) |
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you need the foil off so they can have gas exchange
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MrSpots
Stranger



Registered: 10/02/08
Posts: 10
Last seen: 14 years, 6 months
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Re: whats wrong? [Re: MrSpots]
#11006999 - 09/05/09 11:28 PM (15 years, 4 months ago) |
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hmm would that affect the my mycelium growth? and 20 ml of water.
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                       Sometimes dreams really DOO come true!                     
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Polk_Audio3
Moon Cricket



Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
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Re: whats wrong? [Re: MrSpots]
#11007002 - 09/05/09 11:29 PM (15 years, 4 months ago) |
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Where did you get you're info on cultivating?
watch the pf tek www.mushroomvideos.com
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MrSpots
Stranger



Registered: 10/02/08
Posts: 10
Last seen: 14 years, 6 months
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Re: whats wrong? [Re: MrSpots]
#11007007 - 09/05/09 11:30 PM (15 years, 4 months ago) |
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i got it from http://www.fungifun.org/English/Pftek and ive watched that video plenty of times.
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                       Sometimes dreams really DOO come true!                     
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UFOz8MyGoat
The Governator Sayz Go Chagaz



Registered: 07/18/02
Posts: 1,924
Loc: USA
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Re: whats wrong? [Re: MrSpots]
#11007017 - 09/05/09 11:32 PM (15 years, 4 months ago) |
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ya and it could give mold/bacteria the upper hand
an easy way to remember the mixture is 1 part water / 1 part BRF ./ 3 part verm using a 1/2 pint this makes like 5/6 jars ... It's better to be on the dry side than the wet side because of contams
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GlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
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Re: whats wrong? [Re: MrSpots]
#11007031 - 09/05/09 11:35 PM (15 years, 4 months ago) |
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Yeah, your ratios were way off.
2:1:1
Verm:BRF:water
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MrSpots
Stranger



Registered: 10/02/08
Posts: 10
Last seen: 14 years, 6 months
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Re: whats wrong? [Re: MrSpots]
#11007046 - 09/05/09 11:37 PM (15 years, 4 months ago) |
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alright thanks guys. ill let you know what happens. ill take the foil off and give you an update. also i forgot to tell you that the room temp is around 70 to 75 degrees fahrenheit.
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                       Sometimes dreams really DOO come true!                     
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Polk_Audio3
Moon Cricket



Registered: 02/16/09
Posts: 7,163
Loc: Amsterdam
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Quote:
GlassApex said: Yeah, your ratios were way off.
2:1:1
Verm:BRF:water
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UFOz8MyGoat
The Governator Sayz Go Chagaz



Registered: 07/18/02
Posts: 1,924
Loc: USA
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Quote:
GlassApex said: Yeah, your ratios were way off.
2:1:1
Verm:BRF:water
yes this is right
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MrSpots
Stranger



Registered: 10/02/08
Posts: 10
Last seen: 14 years, 6 months
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Good news guys! so last night i took the foil off and this morning there's a little spec of mycelium growing. i hope it goes good.
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                       Sometimes dreams really DOO come true!                     
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GlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
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Re: whats wrong? [Re: MrSpots]
#11011398 - 09/06/09 07:54 PM (15 years, 4 months ago) |
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Yeah, leave the foil off completely.
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