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Offlineshaughnny
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What did I do wrong?(pictures)
    #10997905 - 09/04/09 09:51 AM (15 years, 4 months ago)

Originally there were 30 1/2 pint BRF jars, about 3 days after inoculation 15 of them germinated and began to colonize. The other 15 jars have not shown any sign of anything since day one....accept for one jar that was growing the dreaded forest mold (Trichoderma)
Pictured below.


So needless to say that one has been tossed in the trash!

Also one of the jars that was colonizing began to show trichoderma, and now the trichoderma has all but colonized this jar...is there no hope for this jar?? Should I just toss it? Just a side note on this jar, there was more moisture in this jar for some reason, not sure why, none of the others are. Here is the jar in question.........


Taken over this side of the jar completely


The rest of the jars eh at did begin colonizing have continued to do so at a slow rate, and some of them are at near 80 percent colonization while some others are at maybe 05 percent. Here is a few of the more progressed jars,



The last photos were taken 3 days ago I think and they have come along way since. So I am left with 14 jars that look like there is no activity. It's been over two weeks since inoculation....are these jars dead? I have more spore syringes....would it hurt to inoculate the same jars again with a new syringe? Or do I start over? Any advise would be terrific.

zoom zoom (:

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Invisiblesimbad42
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10997920 - 09/04/09 09:56 AM (15 years, 4 months ago)

was it multi spore? if so this is normal


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Offlineshaughnny
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Re: What did I do wrong?(pictures) [Re: simbad42]
    #10997930 - 09/04/09 09:59 AM (15 years, 4 months ago)

I bought the spore syringes from a vendor on the internet.Not real sure what multi spore is, still got alot to learn. Should I toss the other contaminated jar out?

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Offlineratatonic30
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10997939 - 09/04/09 10:01 AM (15 years, 4 months ago)

Yes, toss it out. Do it right now!


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Existence always has an Order, called Chaos when incompatible with a chosen good. ~ Some philosopher dude

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InvisibleShea25
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Re: What did I do wrong?(pictures) [Re: simbad42]
    #10997940 - 09/04/09 10:01 AM (15 years, 4 months ago)

Mulitspore has nothing to do with it. It must be either a contamed syringe or your sterile procedures. Did you flame sterilize the needle between each jar??

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Offlineshaughnny
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Re: What did I do wrong?(pictures) [Re: Shea25]
    #10997948 - 09/04/09 10:03 AM (15 years, 4 months ago)

Quote:

Shea25 said:
Mulitspore has nothing to do with it. It must be either a contamed syringe or your sterile procedures. Did you flame sterilize the needle between each jar??



I flame sterilized between each jar and followed the instructions to a tee...

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Offlineratatonic30
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Re: What did I do wrong?(pictures) [Re: ratatonic30]
    #10997956 - 09/04/09 10:04 AM (15 years, 4 months ago)

Also, if you're planning on reinoculating the jars with no growth, PC them first. Your best bet would be to start over from scratch though.


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Existence always has an Order, called Chaos when incompatible with a chosen good. ~ Some philosopher dude

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Offlineshaughnny
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Re: What did I do wrong?(pictures) [Re: ratatonic30]
    #10997960 - 09/04/09 10:04 AM (15 years, 4 months ago)

Quote:

ratatonic30 said:
Yes, toss it out. Do it right now!





Okay I threw it in the trash outside. Thanks for the advice.

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InvisibleShea25
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Re: What did I do wrong?(pictures) [Re: Shea25]
    #10997967 - 09/04/09 10:05 AM (15 years, 4 months ago)

I take it there were 3 syringes used since you said 30 jars to start with and its 1 cc per jar. Chances are 1 of the syringes were contaminated. Also throw those contamed jars away there are of no use anymore

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Offlineshaughnny
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10997972 - 09/04/09 10:06 AM (15 years, 4 months ago)

Do you think I should wait on those jars to see if they will germinate? Maybe wait one more week....I have tons of jars laying around, whenever I want to start again.

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InvisibleShea25
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Re: What did I do wrong?(pictures) [Re: ratatonic30]
    #10997973 - 09/04/09 10:07 AM (15 years, 4 months ago)

I would not advice reinoculating the jars that have no signs of growth. Reinoculating waste of time always do it from scratch

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Offlineshaughnny
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10997982 - 09/04/09 10:08 AM (15 years, 4 months ago)

Yes I used three syringes for thirty jars, Equador, Tapalpa, and Wolongong.
1 cc per jar.

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InvisibleShea25
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10998001 - 09/04/09 10:12 AM (15 years, 4 months ago)

Yea good chances are that one is contamed, A multispore syringe is simply  any spore syringe bought online. It is a syringe full of spores and sterile water, the genetics are random you never know what you with get when it come to growth perimeters. But mulitspore has no affect on the reason it contamed

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OfflineTsalagiSpirit
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10998006 - 09/04/09 10:14 AM (15 years, 4 months ago)

Wait about 14 days after inoculation before tossing/reinnoculating a jar, I've had spores that took 11 days to show signs of life.


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InvisibleShea25
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Re: What did I do wrong?(pictures) [Re: TsalagiSpirit]
    #10998014 - 09/04/09 10:15 AM (15 years, 4 months ago)

He has been waiting over 2 weeks already

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Offlineshaughnny
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10998023 - 09/04/09 10:16 AM (15 years, 4 months ago)

Yeah it's been two weeks......do I wait one more week? Just seems so bizarre that the others are all growing so fast.

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Offlineratatonic30
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10998029 - 09/04/09 10:18 AM (15 years, 4 months ago)

There's no harm in waiting longer if you don't need the jars right now.

Edit - Although, I can't promise anything good will come of it.


--------------------
Existence always has an Order, called Chaos when incompatible with a chosen good. ~ Some philosopher dude

Edited by ratatonic30 (09/04/09 10:19 AM)

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InvisibleShea25
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10998031 - 09/04/09 10:18 AM (15 years, 4 months ago)

I would start over if it has been over 2 weeks. That and you for sure have a comtamed syringe in the mix. Start over getting a syringe from a sponsored site

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Offlinethe_gardener
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Re: What did I do wrong?(pictures) [Re: Shea25]
    #10998299 - 09/04/09 11:20 AM (15 years, 4 months ago)

In my very limited experiance, you have either gotten an unstrile syringe or had a mishap with you strile technique. As forThe no growthers, it might be a good idea to open one outside your house( in a secure location of corse) and take a wiff. If its foul smelling you might have a bactiria and that could be causeing your slow or no groth. Also I was under the impression that when you have a dry verm layer on top of your jars that you should not flip them upside down because you might mix your sub with the dry layer. Either way it looks like you need to either reevaluate who you bought the syringes from or your teks. My turtle for a while had a run of really wet sub and he just ajusted his formula and started having success. And come to think of it he was having some of the same problems you are having. So just my two cents. Let me know if any of this helps.

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Offlinenastos
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Re: What did I do wrong?(pictures) [Re: shaughnny]
    #10998796 - 09/04/09 01:01 PM (15 years, 4 months ago)

quit playing with your jars just let em sit if your using a dry verm layer you are probably strring up the caught contams by tipping it sideways like that


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