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OfflineNsomnia
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First grow ready to scale up: precautionary measures?
    #10996426 - 09/04/09 12:39 AM (15 years, 4 months ago)

I inoculated 7 pint jars with B+ a couple months ago. Six jars colonized a couple weeks ago. One more jar is finishing.

I have 4 jars dunked and spawned 2:1 to pasteurized cpoo with a little verm and just a touch of coffee. Put into a foil tray in a shallow tupperware with 2 inches of perlite on the bottom. Was let to colonize then 'cased' with one more jar followed by peatmoss and verm. The casing laying is turning white but no knots yet. One corner did turn blue/green presumibly trich so I soaked it with a bleach solution and h202 and then cut it out and filled with verm.

Second tupperware same deal but I just puta couple 'wbs cakes' rolled in verm peatmoss and a tiny cpoo. Holes stuffed with polyfill on five sides on both tupperwares humidity is 92% and im fanning frequently with the odd mist.

One jar is spawned 1:1 to cpoo and 1/10 verm with a verm layer on top. In a closed container colonizing.

What should I do with the last jar to gaurentee I have at least a few mushrooms to collect spore prints from to start my next grow? Ill be starting headfirst with agar. I have a glovebox ive been using and ready to upgrade to tyvek coveralls and up to quart jars.

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #10996483 - 09/04/09 12:54 AM (15 years, 4 months ago)

check the links in my sig to see what you need.


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OfflineHaRo On KoRn
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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #10996901 - 09/04/09 02:10 AM (15 years, 4 months ago)

u just gotta wait it out and see what hapens
my foaf really he is well sorta a friend now sorta

any way i got my spores from him
he said he had a cake that took 6 weeks after birth too fruit

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OfflineNsomnia
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11002643 - 09/05/09 02:01 AM (15 years, 4 months ago)

I woke up today to 3 tiny pins and one large one. Added more water to perlite for more humidity and gonna run a lot more light now.

This is on WBS 'cakes' im really glad i did them just to try so I dont expect much but i will dunk and maybe roll in manure then verm again.

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Offlinedarkestmage
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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11002693 - 09/05/09 02:18 AM (15 years, 4 months ago)

u r fuct

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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11002801 - 09/05/09 03:11 AM (15 years, 4 months ago)

Quote:

Nsomnia said:
I woke up today to 3 tiny pins and one large one. Added more water to perlite for more humidity and gonna run a lot more light now.

This is on WBS 'cakes' im really glad i did them just to try so I dont expect much but i will dunk and maybe roll in manure then verm again.





Please, do not roll your cakes in manure..


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OfflineNsomnia
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Re: First grow ready to scale up: precautionary measures? [Re: nastos]
    #11002830 - 09/05/09 03:24 AM (15 years, 4 months ago)

Theyre only value is to create spores to be used to start (successful this time) LC's and more jars. I figure they need some nutrients. cpoo will just mold or take too long to recolonize or...?

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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11002836 - 09/05/09 03:29 AM (15 years, 4 months ago)

poo is for bulk not for cakes u dont want to be rolling your cakes in anything that rich in nutrients it will invite contams for sure


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:shrug:

Edited by nastos (09/05/09 03:35 AM)

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OfflineHaRo On KoRn
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Re: First grow ready to scale up: precautionary measures? [Re: nastos]
    #11002944 - 09/05/09 04:54 AM (15 years, 4 months ago)

CONTAMAS

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OfflineNsomnia
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11005183 - 09/05/09 04:38 PM (15 years, 4 months ago)

Makes sense. Some additional reading verified that. Im just glad I have some fruit bodies to print. 7 pins last night. 12-13 today after i stuck them in the sun for a few hours.

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11005302 - 09/05/09 05:12 PM (15 years, 4 months ago)

Natural Sunlight:sun:

:headbang3:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007539 - 09/06/09 01:17 AM (15 years, 4 months ago)

do cakes grow knots before pins?

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007607 - 09/06/09 01:35 AM (15 years, 4 months ago)

doesnt matter what you have your myc growing on its the life cycle of the fungus, YES, hyphal knots b4 primordia then pins then fruitbody.

13:mushroom2:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007639 - 09/06/09 01:44 AM (15 years, 4 months ago)

what do the knots look like i think my cake has som

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007689 - 09/06/09 02:00 AM (15 years, 4 months ago)

just dots on the myc like white chicken pox.



you can see the brighter tiny white specks. hyphal knots:thumbup: 
the ones that are brown/rust color are primordia.


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007757 - 09/06/09 02:28 AM (15 years, 4 months ago)

so primordia are the ones i want?

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007772 - 09/06/09 02:36 AM (15 years, 4 months ago)

its the life cycle of the mushroom,

1) spores -2) spore germination -3) fusion of compatable hyphae -4) hyphal knot -5) pinhead -6) primordium -7) small mushroom -8) mature fruitbody -back to 1) spores.

its full circle, you have to have one in order to get the other.:thumbup:

Pg.5 Figure 2. in The Mushroom Cultivator by Paul Stamets. (just so you know Im not talking out of my ass)

13:mushroom2:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007810 - 09/06/09 02:53 AM (15 years, 4 months ago)

ok so dam this is my first grow

i want it to grow so bad

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007835 - 09/06/09 03:03 AM (15 years, 4 months ago)

if you have patience, you will have shrooms :waits:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007846 - 09/06/09 03:06 AM (15 years, 4 months ago)

o i got the waiting down i got a mono tub with hpoo and 2 brf cakes colonizing right now almost 2 weeks in at 25% gonna take a long while for these

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007854 - 09/06/09 03:08 AM (15 years, 4 months ago)

how big is your mono?  better yet how much substrate did you spawn to?


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007877 - 09/06/09 03:17 AM (15 years, 4 months ago)

44 quart like 1 inch thick like hmm 2-3 quarts of poo

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007897 - 09/06/09 03:25 AM (15 years, 4 months ago)

try to keep your substrate to spawn ratio low like 1:2 or 1:4.  the more spawn the faster it colonizes the better the chance of not getting any contams.  if you have 3 quarts of sub you should have at least 1 quart spawn.  you have a pint.  4-5 1/2pint cakes would give you a quart of spawn.  I have 18gal monos with 3"-4" sub depth and use around 12 quarts sub and 6 quarts spawn. 1:2 ratio.  Ive used less and it takes longer to colonize and mold sets in b4 a second flush all the time.

I hope your tub is sealed up for gas exchange only and your not opening it to check on it:eek:
you should have a view port cut out in your lid covered in clear plastic or plexi glass so you dont have to expose your uncolonized sub to freash air too early giving you a very uneven pinset and/or low yeild.:stoned: 

just a heads up incase you already havent done so.:thumbup:

13:mushroom2:


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OfflineHaRo On KoRn
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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007921 - 09/06/09 03:34 AM (15 years, 4 months ago)

i have it covered checked on it twice but thanks for the info i will check a lot less

also i want to make mono 2 litres do u think 1 pint of hpoo and 1/2 pint spawn will make a good mini 2 litre mono

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007932 - 09/06/09 03:40 AM (15 years, 4 months ago)

yup,:sun:
Ive used 1gal. milk jugs for mini shotgun FCs b4, got 3 cakes and 4" of perlite all to fit inside.  it worked:thumbup:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007936 - 09/06/09 03:43 AM (15 years, 4 months ago)

hmm maybe if i make a mono out of a milk carten it would work better

how did u make the lid im thinken cranwrap

o have u ever inculated wbs with a transfer for a brf jar

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007939 - 09/06/09 03:46 AM (15 years, 4 months ago)

Quote:

HaRo On KoRn said:
what do the knots look like i think my cake has some





if you see knots then it will be less than a week and you will be picking:mushroomgrow::mushroomgrow::mushroomgrow:

just to let you know.:thumbup:


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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007950 - 09/06/09 03:53 AM (15 years, 4 months ago)

Quote:

HaRo On KoRn said:
hmm maybe if i make a mono out of a milk carten it would work better

how did u make the lid im thinken cranwrap

o have u ever inculated wbs with a transfer for a brf jar




I used straws to form a structor and then draped plastic wrap over/around for a lid.

yes, use many small pieces of myc in each transfer for faster colonization.  I did that exact thing with the cakes in my milk jug.



you can see where I cut it away with a scalpel, it recovered for a second flush.:smirk:



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OfflineHaRo On KoRn
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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11007955 - 09/06/09 03:55 AM (15 years, 4 months ago)

thats sweet thanks so much for the help

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OfflineNsomnia
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11007997 - 09/06/09 04:35 AM (15 years, 4 months ago)

I agree great words. I am going to take my last jar and crate your 18gal monotub. then just forget about it.

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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11010335 - 09/06/09 04:09 PM (15 years, 4 months ago)

if u do that ull come back 3 months later with moldy shrooms

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: HaRo On KoRn]
    #11010896 - 09/06/09 06:05 PM (15 years, 4 months ago)

Quote:

try to keep your substrate to spawn ratio low like 1:2 or 1:4.  the more spawn the faster it colonizes the better the chance of not getting any contams.  if you have 3 quarts of sub you should have at least 1 quart spawn.  you have a pint.  4-5 1/2pint cakes would give you a quart of spawn.  I have 18gal monos with 3"-4" sub depth and use around 12 quarts sub and 6 quarts spawn. 1:2 ratio.  Ive used less and it takes longer to colonize and mold sets in b4 a second flush all the time.





13:scaryshroom::thumbup:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11012419 - 09/06/09 11:42 PM (15 years, 4 months ago)

My tray of cpoo + verm + 2 pints followed by 1 pint spread on top then a verm/peat casing finally has one tiny tiny pin.

I hope they explode soon... I expected dozens to show up at once.

Should my casing layer be moist? The substrate is 100% wet but the casing layer is dry-dry-damp.

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Invisible13shroomsM
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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11012428 - 09/06/09 11:46 PM (15 years, 4 months ago)

sounds good, next time try mixing your spawn with your sub then put the casing on, makes for a better flush due to the myc colonizing more of the sub getting more nutes.:stoned:

a mist once in a while to give it a "glistening" look but no BIG drops or puddles will give your pins a nice drink. :mushroomgrow: 

If it looks dry.....mist, if you can visibly see moisture.....fan.:wink:

its a good rule of thumb:thumbup:


13:mushroom2:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11012517 - 09/07/09 12:13 AM (15 years, 4 months ago)

Thats what ive been reading great help thanks. I did mix the spawn with the substrate it colonized super fast. it was like 1:1 or 1:2.

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Re: First grow ready to scale up: precautionary measures? [Re: Nsomnia]
    #11012524 - 09/07/09 12:14 AM (15 years, 4 months ago)

:scaryshroom::thumbup:


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Re: First grow ready to scale up: precautionary measures? [Re: 13shrooms]
    #11013235 - 09/07/09 06:10 AM (15 years, 4 months ago)

i wish i knew this

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