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200proofhonky
bluethumb



Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
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Clone problems
#10993622 - 09/03/09 04:57 PM (15 years, 4 months ago) |
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I have a clone Lc ive been using for 3-4 months successfully, the flushes and fruits were great and i got at least 4, if not 5 flushes out of every mono. Now for some reason,my last 3-4 mono's have had shitty flushes and they are all mutants (tiny and mutated).
This clone was made by introducing a piece of tissue to a wbs jar in a glove box.
All my conditions are the exact same as before, i have a master LC so it cant be degenerating can it?
What are some things that would cause this?
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They try to take away everything God gave us,yet...
our money still says "In God We Trust".
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You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
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If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.
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FoxFire
Energized Matter



Registered: 07/17/05
Posts: 1,281
Loc: PNW
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Is your LC master being stored in the fridge?
-------------------- Unless someone like you cares a whole awful lot,
Nothing is going to get better. It's not.
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 5 years, 6 months
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Re: Clone problems [Re: FoxFire]
#10993802 - 09/03/09 05:39 PM (15 years, 4 months ago) |
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--------------------
 
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-pEaCeLoVeGoDbLeSs-
"The Downfall of Mankind; is Believing He Has Limitations."
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Clone problems [Re: just me]
#10994027 - 09/03/09 06:16 PM (15 years, 4 months ago) |
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Contam lurking in the master LC, maybe.
-------------------- You make it all possible. Doesn't it feel good?
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200proofhonky
bluethumb



Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
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Re: Clone problems [Re: Doc_T]
#11005364 - 09/05/09 05:34 PM (15 years, 4 months ago) |
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Quote:
ManaShaman said: Is your LC master being stored in the fridge?
yes, its in the fridge until i need it,then i pull it out a few days before i use it to get it growing again.
Quote:
Doc_T said: Contam lurking in the master LC, maybe.
no cantam's, im still getting 4-5 flushes before it goes green.
-------------------- ------------------------------------------------------------------------------------------------------------------------------
They try to take away everything God gave us,yet...
our money still says "In God We Trust".
------------------------------------------------------------------------------------------------------------------------------
You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
------------------------------------------------------------------------------------------------------------------------------
If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.
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200proofhonky
bluethumb



Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
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Re: Clone problems [Re: just me]
#11005368 - 09/05/09 05:35 PM (15 years, 4 months ago) |
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Quote:
just me said:

lol, whats the ninja about?? sneaky contams? lol
-------------------- ------------------------------------------------------------------------------------------------------------------------------
They try to take away everything God gave us,yet...
our money still says "In God We Trust".
------------------------------------------------------------------------------------------------------------------------------
You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
------------------------------------------------------------------------------------------------------------------------------
If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 5 years, 6 months
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its my way of slipping in and out nearly un-noticed
just like a ninja, silently watching, listening...
--------------------
 
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-pEaCeLoVeGoDbLeSs-
"The Downfall of Mankind; is Believing He Has Limitations."
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200proofhonky
bluethumb



Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
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Re: Clone problems [Re: just me]
#11005713 - 09/05/09 07:20 PM (15 years, 4 months ago) |
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ahh, i see.... weird, but whatever, LOL
-------------------- ------------------------------------------------------------------------------------------------------------------------------
They try to take away everything God gave us,yet...
our money still says "In God We Trust".
------------------------------------------------------------------------------------------------------------------------------
You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
------------------------------------------------------------------------------------------------------------------------------
If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Anything new in your setup? a new misting bottle or nething like that?
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: Clone problems [Re: anonjon]
#11005782 - 09/05/09 07:42 PM (15 years, 4 months ago) |
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It sounds a lot like virus. You never know with LC until you use it. I've also never had luck with long-term storage using LC. Plain distilled water will preserve mycelium for years, but a nutrient solution doesn't seem to hold up as well. I'd suggest master slants for storage. Inoculate some petri dishes for each cycle, and then use those to inoculate your grains. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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NineInchNails
Stranger

Registered: 03/01/09
Posts: 1,190
Last seen: 2 years, 12 days
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Quote:
RogerRabbit said: It sounds a lot like virus. You never know with LC until you use it. I've also never had luck with long-term storage using LC. Plain distilled water will preserve mycelium for years, but a nutrient solution doesn't seem to hold up as well. I'd suggest master slants for storage. Inoculate some petri dishes for each cycle, and then use those to inoculate your grains. RR
Really? ! ? !
So do you recommend a Grain LC specifically for LONG term storage inside a refrigerator?
I always like to keep spore prints as a last resort backup, but I’m in the habit of using LCs for inoculations. It wouldn't be too much to ask to donate at least one grain jar for grain LC.
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200proofhonky
bluethumb



Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
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Quote:
RogerRabbit said: It sounds a lot like virus. You never know with LC until you use it. I've also never had luck with long-term storage using LC. Plain distilled water will preserve mycelium for years, but a nutrient solution doesn't seem to hold up as well. I'd suggest master slants for storage. Inoculate some petri dishes for each cycle, and then use those to inoculate your grains. RR
I never knew that virus could infect fungus, im gonna research that a little.
I may just start using grain LC, i know slants are the best but i dont have a flow hood and it just seems like an awful lot to go through trying to do all that in a glove box.
thanks RR
-------------------- ------------------------------------------------------------------------------------------------------------------------------
They try to take away everything God gave us,yet...
our money still says "In God We Trust".
------------------------------------------------------------------------------------------------------------------------------
You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
------------------------------------------------------------------------------------------------------------------------------
If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.
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