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tadziu
Monster Maker

Registered: 08/21/09
Posts: 99
Last seen: 2 years, 11 months
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Worms/ larvae in Jars... sources and solutions **TRUSTED GROWERS PLS**
#10992466 - 09/03/09 01:06 PM (15 years, 4 months ago) |
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I've found some crawling larvae in a couple of my PF tek jars where I used Dark Rye Flour. I sterilized for over 90 mins and ensured that I had as sterile conditions as possible (steamed jars for 90-120 mins, alcohol sterilized everything, flame sterilized needles, glove box, face mask, clean environment, etc) so not sure how this could have occurred. I suspect these jars are trash now? Who knows (yet), maybe more or all jars are infected.
1) Can the flies only get in the jars thru the inoculation & gas exchange holes if they are the only source of entry, providing that the jars were originally sterlized properly and killed everything inside before inoculation?
2) If micropore tape is left on can they still get in to lay eggs?
3) Can I still save some mycelium and grown in agar?
Cheers!
Edited by tadziu (09/03/09 06:28 PM)
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Miscusi
Upstate Chillin'




Registered: 07/02/04
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Re: Worms/ larvae in Jars... sources and solutions [Re: tadziu]
#10992482 - 09/03/09 01:09 PM (15 years, 4 months ago) |
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Quote:
tadziu said: I've found some crawling larvae in a couple of my PF tek jars where I used Dark Rye Flour. I sterilized for over 90 mins and ensured that I had as sterile conditions as possible (steamed jars for 90-120 mins, alcohol sterilized everything, flame sterilized needles, glove box, face mask, clean environment, etc) so not sure how this could have occurred. I suspect these jars are trash now? Who knows (yet), maybe more or all jars are infected.
1) Can the flies only get in the jars thru the inoculation & gas exchange holes if they are the only source of entry, providing that the jars were originally sterlized properly and killed everything inside before inoculation?
2) If micropore tape is left on can they still get in to lay eggs?
3) Can I still save some mycelium and grown in agar?
Cheers!
I don't have an answer for you. All I can say my friend is I've been growing for the better part of 5 years. And browsed this board since 2004. I can say IVE Never see or herd of any experiencing that problem.
Im amazed honestly. of all things to grow or contam your jar.....
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tadziu
Monster Maker

Registered: 08/21/09
Posts: 99
Last seen: 2 years, 11 months
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Re: Worms/ larvae in Jars... sources and solutions [Re: Miscusi]
#10992537 - 09/03/09 01:18 PM (15 years, 4 months ago) |
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Me neither Miscusi; however, I found this thread http://www.shroomery.org/forums/dosearch.php?terms=worms+in+jars.
One thing to take away from this and take into consideration when removing tape from holes is that your cakes/ jars could potentially become infected by worms... if this is the cause.
Anyway, it's not new or impossible, but I'm still looking for answers to my questions!
-------------------- This forum is here to discuss issues and to HELP other members...
If your answer to a posted question is simply going to be "use the search function" then why not make your post worthwhile and useful by providing them with a link that points them in the right direction... otherwise, keep it to yourself!
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electrics
wave rider



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Re: Worms/ larvae in Jars... sources and solutions [Re: tadziu]
#10992604 - 09/03/09 01:30 PM (15 years, 4 months ago) |
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I have no explanation for you're situation did you include a dry verm barrier in you're jars?? also after inoculation it is a good Idea to cut tyvek circles and rubber band them to the tops of jars allowing Fresh Air Exchange and keeping nastiest out!!the only thing I can think of is the larvae survived the sterilization technique used and know have infested you're stuff..It is possible if they are 100% colonized to dunk them in a Hydrogen Peroxide dunk and then roll in verm and hope for the best!....good luck.....e
-------------------- "Listen now I'm talking I've been here for weeks, waiting in this growing crowd staring at my feet, The world around me is Turning I'm just standing still, The time has come for changes do something or I will" Phish
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solumvita
Q.B.E.


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Re: Worms/ larvae in Jars... sources and solutions [Re: electrics]
#10992636 - 09/03/09 01:37 PM (15 years, 4 months ago) |
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sounds like you have some maggot eggs that may have survived your cooking. Were the jars packed tightly into the PC?
Other than that you have some flies crawling and laying eggs.
Either way I have not really seen flies cause major damage to mushrooms especially not on a small scale. I would not worry to much when you birth it just pick them off with some forceps and squash them.
-------------------- One of these days all the answers will be revealed until then we learn from each other!
www.mushrush.co.za
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tadziu
Monster Maker

Registered: 08/21/09
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Last seen: 2 years, 11 months
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Re: Worms/ larvae in Jars... sources and solutions [Re: solumvita]
#10992851 - 09/03/09 02:24 PM (15 years, 4 months ago) |
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I didn't use a PC, I steam sterilized for 90-120 mins, as outlined in the PF tek and videos by RR and RK. Basically, I followed these videos and PF tek instructions down to a "T".
1) Yes, inside rims of jars wiped clean and dry and dry verm layer applied. Micropore tape over the 4 holes. Jars sealed tight.
2) Jars not PC'd but steamed in canning pot for 90-120 mins. No, put not packed tight at all, as I only had 6 jars in at a time.
3) Covered jars with foil prior to sterilization tho removed after sterilization & just before putting into glove box for immediate inoculation;
4) Before jars were inoculated in glove box they were wiped down with rubbing alcohol for extra safety measure;
5) Inoculated jars again wiped down with rubbing alcohol prior to and after incubation;
6) After 1-2 weeks removed tape because of no signs of mycelium growth. I've subsequently put new tape over the holes to prevent any further spread of the infection (which may or may not be too late);
A week or 2 ago I did, however, notice a fruit fly around my jars... perhaps it could be the f*cker! However, would dry verm layer, etc not prevent its eggs from entering? Possibly not. Guess a warning to others out there who are thinking about removing the tape from the holes!
As for worms not causing too much damage... I heard that they feed off the mycelium and can devastate the jar so best to throw away http://www.shroomery.org/forums/dosearch.php?terms=worms+in+jars ... if this is in fact correct.
I got the syringes from a previous but no longer Shroomery vendor. I don't want to cast blame but perhaps this could also be the source of contamination.
To save some credibility here I won't tell you how I accidentally broke one of the infected jars; however, I want to try and save the mycelium so is it possible to take samples of the mycelium and grow in agar or is it a lost cause?
I've ordered from Ralphsters this time so we'll see if there are any differences between the vendors' products. So far have not been happy at all with the speed of colonization. All of the optimum incubation parameters have been met and maintained but after 2-3 weeks my most successful (and now lost) jar was only at about 10%.
Cheers folks
-------------------- This forum is here to discuss issues and to HELP other members...
If your answer to a posted question is simply going to be "use the search function" then why not make your post worthwhile and useful by providing them with a link that points them in the right direction... otherwise, keep it to yourself!
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electrics
wave rider



Registered: 12/02/08
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Re: Worms/ larvae in Jars... sources and solutions [Re: tadziu]
#10994194 - 09/03/09 06:43 PM (15 years, 4 months ago) |
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I'm gonna tell you something that you don't want to hear..buy a PC!! Sorry man I know people have been successfully with just boiling..but if you are serious about this hobby get yourself a nice pressure cooker if you can't afford a nice one right now find a cheap one on e-bay who cares if it only does 4 jars at a time it will just take longer but believe me the end results will be 100% better!! specially working with brf..it is the way to go!!...e
-------------------- "Listen now I'm talking I've been here for weeks, waiting in this growing crowd staring at my feet, The world around me is Turning I'm just standing still, The time has come for changes do something or I will" Phish
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ABC
Stranger



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Re: Worms/ larvae in Jars... sources and solutions [Re: electrics]
#10995315 - 09/03/09 09:17 PM (15 years, 4 months ago) |
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A PC is not necessary for PF tek. There's no way fly eggs could have survived. It is highly likely that the flies laid their eggs after you removed the tape. though they may be able to lay eggs through the tape, but I'm not sure
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tall dwarf
Strange
Registered: 06/11/09
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Re: Worms/ larvae in Jars... sources and solutions [Re: ABC]
#10995689 - 09/03/09 10:20 PM (15 years, 4 months ago) |
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Most of what is written in this thread is incorrect. The gnats get inside the jars post-sterilization through the gas exchange holes, and YES micropore tape will keep them out. I don't know how bad your infestation problem is but those jars can still be used.
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tadziu
Monster Maker

Registered: 08/21/09
Posts: 99
Last seen: 2 years, 11 months
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Re: Worms/ larvae in Jars... sources and solutions [Re: tall dwarf]
#10995851 - 09/03/09 10:48 PM (15 years, 4 months ago) |
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I agree folks, I think it's post sterilization and thru the inoc holes. There's really no way a fly can get thru that tape so it would obviously have to be when the tape was removed. Lesson here for everybody, I suppose. Little bastards!
Golden_Student you said that those jars can still be used... what do you mean by this? FYI, I only saw one worm in each of the 2 jars so I don't think it's an infestation per se.
That being said, I still can't figure out the issue with all of my jars colonizing so slowly. I'm assuming the worms had nothing to do with it; otherwise, I'm screwed with all the other jars!
-------------------- This forum is here to discuss issues and to HELP other members...
If your answer to a posted question is simply going to be "use the search function" then why not make your post worthwhile and useful by providing them with a link that points them in the right direction... otherwise, keep it to yourself!
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RogerRabbit
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Re: Worms/ larvae in Jars... sources and solutions [Re: tadziu]
#11010601 - 09/06/09 05:04 PM (15 years, 4 months ago) |
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This is pretty common. Flies can lay eggs through the micropore tape and into the jars. If there's a huge fly population in your house, I'd suggest building a screened in box to keep your colonizing jars in. RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
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Dionili
Second Rate Mycologist



Registered: 08/18/09
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Re: Worms/ larvae in Jars... sources and solutions [Re: RogerRabbit]
#11020466 - 09/08/09 12:08 PM (15 years, 4 months ago) |
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Quote:
RogerRabbit said: This is pretty common. Flies can lay eggs through the micropore tape and into the jars. If there's a huge fly population in your house, I'd suggest building a screened in box to keep your colonizing jars in. RR
That and clean your house/grow room..
BUgs....nastys..
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Buddhapaper
Stranger


Registered: 07/10/09
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Re: Worms/ larvae in Jars... sources and solutions [Re: Dionili]
#11020974 - 09/08/09 01:32 PM (15 years, 4 months ago) |
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Thats pretty gross.
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tadziu
Monster Maker

Registered: 08/21/09
Posts: 99
Last seen: 2 years, 11 months
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Re: Worms/ larvae in Jars... sources and solutions [Re: Buddhapaper]
#11026423 - 09/09/09 10:16 AM (15 years, 4 months ago) |
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Thanks RR. Nobody ever seems to really make mention this and I had never heard of this before so it was a shock when I saw it crawling in my jar.
FYI, my place is really clean and the one fly I mentioned seeing was the first and only fly I saw.
-------------------- This forum is here to discuss issues and to HELP other members...
If your answer to a posted question is simply going to be "use the search function" then why not make your post worthwhile and useful by providing them with a link that points them in the right direction... otherwise, keep it to yourself!
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