Home | Community | Message Board

The Spore Depot
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblecyb3rtr0n
searching for truth
Male User Gallery


Folding@home Statistics
Registered: 05/06/09
Posts: 1,832
Panaeolus (Copelandia) cambodginiensis on on Agar
    #10992165 - 09/03/09 11:52 AM (15 years, 4 months ago)

Working with Panaeolus (Copelandia) cambodginiensis on agar.  My first time working with agar, so I am trying to  get accustom to what I should be seeing at different stages.

Started off with eight petri dishes of light malt extract agar, using one drop of spores from a multispore syringe. Out of the eight the following three are all that show growth after nine days. the ones that don't show any growth have excess moisture on the surface of the agar that I can see run when I tilt the petri dish.

I believe I poured the agar a little too thick in the dish, and the surface I pored them on wasn't level. So, one side of the dish is thicker than the other.

How much longer should I wait before making my first set of transfers?



This dish I had one drop at the needle tip, but I streaked the needle onto the agar in a Z pattern.  My only problem is that the mycelium is getting close to the top edge now.  I think if I do streak onto the agar again, I will just do one line in the center of the dish.  This dish showed the first signs of growth






I currently only have a non macro manual focus lens for my camera, hence the picture quality.


--------------------
LAGM v.2.024 Grow log

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Using classroom agar petri dishes? Stevo21 976 3 09/07/05 02:19 PM
by jjam
* Re: Agar & Petri Dish Problem MrBoomer 2,373 3 04/16/01 09:49 PM
by Anno
* AGAR AND STUFF......LOADS OFF QUESTIONS......LOL smokes 1,730 1 11/17/02 12:07 PM
by Zero7a1
* agar newbie question KafkaPie 1,712 10 05/18/03 09:50 PM
by micro
* old agar dish boomer420 1,354 8 04/14/04 07:08 PM
by thisspock
* sterilizing petri dishes llamaboy 10,959 11 01/17/04 02:45 PM
by ragadinks
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,904 38 05/11/01 11:08 AM
by MAIA
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,722 8 02/14/05 07:57 AM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,235 topic views. 22 members, 91 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.018 seconds spending 0.007 seconds on 11 queries.