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Offlinecanon7
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Help with spores...
    #10991804 - 09/03/09 10:35 AM (15 years, 4 months ago)

Hey guys I just ordered some Psilocybe cubenis: burma spores from sporeworks.  I ordered 2 single spore kits.  I received the spores, and it doesn't tell me the CC size or anything.  Not sure if the syringes are even in cc's, but the numbers go up to 10, so any idea if they are 10cc syringes?  Also I only see like 2 spores in each syringe, so does that mean I can only inoculate 2 jars?  Sorry for the newb questions.

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InvisibleDoc_T
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Re: Help with spores... [Re: canon7]
    #10991808 - 09/03/09 10:36 AM (15 years, 4 months ago)

Right, 10 mL. Spores are microscopic, there's thousands in there, SporeWorks packs them in.
Shoot w/ confidence. :thumbup:


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Offlinenastos
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Re: Help with spores... [Re: canon7]
    #10991814 - 09/03/09 10:37 AM (15 years, 4 months ago)

You can only see clusters of spores containing 10,000 or more or they arent visible to the naked eye.  Curious as to where you purchased syringes?

edit: its early an i suck at reading


shake the shit out of the syringe and it should be good for 10 or so jars if you use it wisley.


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:shrug:

Edited by nastos (09/03/09 10:40 AM)

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Offlinecanon7
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Re: Help with spores... [Re: nastos]
    #10991885 - 09/03/09 10:51 AM (15 years, 4 months ago)

Ok great, I read to use about 1cc per jar.  Also should I shake the syringe to mix up the spores ?  Also, I'm going to be using the PF Tek method for growing these burma spores.  I have bought Verm & Brown Rice Flour ( Bought the rice, and grinded it in a coffee grinder)  would this be sufficient for this strain?

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Invisibleelemental420
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Re: Help with spores... [Re: canon7]
    #10991913 - 09/03/09 10:57 AM (15 years, 4 months ago)

I used a tad over 1 cc per jar u just dont want to much otherwise your water ratio will b off.

Yes shake your syringe to break the spores up.

and yes you flour will work.

Gl


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Invisiblefeelfunny
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Re: Help with spores... [Re: canon7]
    #10991914 - 09/03/09 10:57 AM (15 years, 4 months ago)

yes shake the syringes. and every thing else is fine just follow the tek and you will be enjoying you mushrooms in a little over a month. good luck


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
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Offlinecanon7
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Re: Help with spores... [Re: feelfunny]
    #10991936 - 09/03/09 11:00 AM (15 years, 4 months ago)

Nice, thanks a lot for the quick responses.  This will be my 2nd attempt at growing cubenis.  I did it last time, but used 1 pint jars, and couldn't get them to colonize.  This time around I'm a little more prepared, 1/2 pint jars, verm & brown rice.  Did a lot of research watched a lot of videos on www.mushroomvideos.com ; so I'm hoping this process goes a lot smoother this time around.

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Invisibleelemental420
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Re: Help with spores... [Re: canon7]
    #10991944 - 09/03/09 11:01 AM (15 years, 4 months ago)

YOu will be fine. Thats about exactly how I had done mine. GL


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Offlinecanon7
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Re: Help with spores... [Re: elemental420]
    #10991967 - 09/03/09 11:06 AM (15 years, 4 months ago)

Ok 1 more question, after you put top off the jars with verm and close them off, I see people put tape on top of the jars to close off the holes.  When I was watching the video, the tape was still on top of the jars, and he inoculated through the tape.  Is this a good procedure or should I remove the tape then inoculate?  Also after inoculation anyone recommend a good place to store the jars, should the be out of sunlight?  I know to keep them between 75-80 degrees.

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Invisiblefeelfunny
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Re: Help with spores... [Re: canon7]
    #10991985 - 09/03/09 11:08 AM (15 years, 4 months ago)

i keep the tape but up to you.


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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Invisibleelemental420
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Re: Help with spores... [Re: canon7]
    #10991992 - 09/03/09 11:10 AM (15 years, 4 months ago)

I shot them up through the tape, then after I had them like 50% colonized I took off the tape for more air circulation.

I think rr said them being in a dark area was just a myth but I just threw mine in a my movie cabinet, it stayed around 80.

Gl <3 Ele


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Offlinecanon7
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Re: Help with spores... [Re: elemental420]
    #10992015 - 09/03/09 11:15 AM (15 years, 4 months ago)

Cool thanks a lot for the responses.  One last question before I go inoculate my jars.  They show in the RR videos that he uses a glove box to inoculate the jars, guessing he is using that to keep bacteria to a minimum.  Do you guys feel this is necessary to make a glove box to inoculate?  If not, anyone recommend a way to inoculate in a clean environment like spray lysol or something lol.

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Re: Help with spores... [Re: canon7]
    #10992035 - 09/03/09 11:20 AM (15 years, 4 months ago)

Not neccessary at all. Do this and you won't be sorry.

Edit: Look at Injecting Spores in the TEK.


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InvisibleechoesSG
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Re: Help with spores... [Re: elemental420]
    #10992038 - 09/03/09 11:21 AM (15 years, 4 months ago)

Quote:

elemental420 said:

I think rr said them being in a dark area was just a myth




Not really a myth, just an old misconception from the old school cultivators.

I leave mine on a shelf near my well lit fruiting chamber and they colonize just fine

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Invisibleelemental420
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Re: Help with spores... [Re: canon7]
    #10992041 - 09/03/09 11:22 AM (15 years, 4 months ago)

do it some where without carpet spray everything down that is around the area u are going to work.

Glove box I do recomend, because it decreases the amount of movement in the air.

But my first time I didnt use one and it worked fine


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Offlinecanon7
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Re: Help with spores... [Re: elemental420]
    #10992061 - 09/03/09 11:27 AM (15 years, 4 months ago)

Moonshine, in your guild it says "Tape can be taken off the holes when colonization reaches 60-70%. At this time the jars should be flipped upside down until they finish colonizing."  Do you feel this is an important step I should not forget?

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Invisiblefeelfunny
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Re: Help with spores... [Re: canon7]
    #10992068 - 09/03/09 11:29 AM (15 years, 4 months ago)

not a big deal just a preference. every one has there thing.


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Invisibleelemental420
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Re: Help with spores... [Re: feelfunny]
    #10992086 - 09/03/09 11:34 AM (15 years, 4 months ago)

Yup what feelfunny said


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Offlinecloudsaregathering
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Re: Help with spores... [Re: elemental420]
    #10992135 - 09/03/09 11:47 AM (15 years, 4 months ago)

i have flipped and not flipped same strain, same LC and both took nearly the same amount of time...


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InvisibleDoc_T
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Re: Help with spores... [Re: cloudsaregathering]
    #10992152 - 09/03/09 11:49 AM (15 years, 4 months ago)

In my opinion the less you handle a jar, the better. That dry verm layer loves to shift around.


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