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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: MycoMal]
    #10996294 - 09/04/09 02:12 AM (12 years, 11 months ago)

Quote:

Sorry for the off subject question but are liberty caps potent?






Quote:

MycoMal said:
This thread has a lot of info on the Liberty Caps. http://www.shroomery.org/forums/showflat.php/Number/2644234





all here...:scaryshroom::thumbup:


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Invisibleelemental420
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #10996371 - 09/04/09 02:25 AM (12 years, 11 months ago)

Thanks and I just got finished reading every ones posts,

Im not sure how much help I will be for you guys still a newb but Ima stick around and watch cuz Im pretty interested.


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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: elemental420]
    #10996410 - 09/04/09 02:34 AM (12 years, 11 months ago)

elemental: aprox 3 times I think.

13: just watch the RR vids man, he covers it pretty good. I take it you are planning on doing the work inside a glovebox, correct? I'll tell you gloveboxes are a flipin pain. Mine is beginning to be a little cloudy (it's a year and a half old) it can be difficult to see the finer definition when it's in the box. I often have to plan and draw out directions for what I'm going to do in sharpie on the bottom of the dish. Ya know marking where the good sectors are so I can tell when it's in the box.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: ScavengerType]
    #10996444 - 09/04/09 02:43 AM (12 years, 11 months ago)

Quote:

13: just watch the RR vids man, he covers it pretty good. I take it you are planning on doing the work inside a glovebox, correct?




I own them, I watch them a couple times a week so that Im going through the motions in my sleep.:thumbup:



Glovebox, check!:stoned:


bioBOT says:
Quote:

Unforseen difficulties aside, I'm guessing
Liberty Cap mushrooms will fruit under fairly
consistent environmental parameters.

Once these parameters are identified, it becomes a
straightfoward task of implementing technology, or
harnessing natural conditions to provide them.

Perhaps a list is in order detailing what aspects
of their cultivation have yet to be identified.

Substrate:          Grass Seed, Organic Compost?
Fruiting Temp:      45-50deg F??
Incubation Temp:    65deg F?
Air Exchange:        Constant?
Fruiting Humidity:  80%+ ??

These are my best guesses... all those who are
willing to share feel free to provide.




from the advanced forum thread.  input???


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Edited by 13shrooms (09/04/09 03:08 AM)


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InvisibleElSeta
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11001423 - 09/04/09 11:12 PM (12 years, 11 months ago)

Where did you get the prints from?


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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: ElSeta]
    #11001569 - 09/04/09 11:41 PM (12 years, 11 months ago)

Quote:

ElSeta said:
Where did you get the prints from?




from a shrommerite in a trade:thumbup:

but I recieved them 2day and they were origanaly from the FSRE (acording to the labeling) so I imagine they have been passed around a few times cuz he told me he got them from a shroomerite trade out of germany.:thumbup:

awaiting my agar supplies now.......

so what does everyone think I should try first for spawn substrate material..I know grass seed, but what else verm for moisture, straw, coffee for Nitrogen? brf/drf, PF-tek, grain/wbs or what? need advice.:thanx:


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Offlinemushroomhunter10
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11001590 - 09/04/09 11:45 PM (12 years, 11 months ago)

I wanted to this.

I'm not sure, but every pic I see of Liberty Caps I've seen it looks as though they are "feeding" off of the grass' root system...

Could they be symbiotic?

I would say definitely start with grains, or even LC>Grains. Then after that it's anybody's guess!


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Invisibledeucedbi9
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: Workman]
    #11002803 - 09/05/09 05:12 AM (12 years, 11 months ago)

Quote:

Workman said:
I used grass seed because it has more hull than other grains.  Liberty caps are associated with grass and the mycelium is seen attached to dead grasses and grass roots. They prefer the nitrogen enriched soils where manure was once present and where grass thrives.  They also seem to tolerate fairly soggy ground.

In my experience, the mycelium grows fairly slowly, especially on sterilized horse manure.  Room temperature seems fine for colonization (around 72F).  Although manure doesn't seem to be an ideal substrate, once fully colonized it pins readily when exposed to unsterile soil casing and cold temperatures.  But the pins don't always mature.  Invitro fruiting is unlikely.




have you considered that libs, may, to some extent, form a symbiotic relationship with a 'benefitial bacteria'?

last year i collected a soil sample from directly beneath one of the three libs i found, in what was once a very productive field.
i failed to get a print though, so had nothing to work with.
might try again this year.

hopefully such a "benefitial bacteria" (in a soil sample) would survive drying, to be later reanimated in either a bulk substrate or  casing mix.
or, at least survive pasteurisation when fresh.


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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: deucedbi9]
    #11004729 - 09/05/09 04:52 PM (12 years, 11 months ago)

The DSMZ Institute of microorganisms from Braunschweig / Germany is using
oat flake agar for some of their Psilocybe semilanceata strains.

Psilocybe semilanceata grows much better on oat flake agar than
on malt extract agar or on potato dextrose agar.

Click on "medium"  (189)

http://www.dsmz.de/microorganisms/html/strains/strain.dsm002331.html

http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium189.pdf


--------------------
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http://www.shroomery.org/forums/showflat.php/Number/11179100


Edited by nightflyer (09/06/09 03:28 AM)


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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: nightflyer]
    #11004784 - 09/05/09 05:01 PM (12 years, 11 months ago)

OAT FLAKE MEDIUM
Oat flakes 30.0 g
Agar 15.0 g
Distilled water 1000.0 ml
Boil oat flakes in water for 10 min; fill up to 1 l and add agar. Autoclave 20 min at
121°C. Shake before pouring.

I an ignorant american,:shrug: can someone tell me what 121C is in Fahrenheit and/or Psi??


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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11004828 - 09/05/09 05:12 PM (12 years, 11 months ago)

121 C = 250 F

http://www.pauliland.ch/zahlenparade/umrechtemperatur/umrechnung-cf.html

Adjust the pH value to 4.5

Beware, this medium will be less firm than other agar recipes, so extra agar has
to be added to compensate. (30 gr. /  1 L)


--------------------
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http://www.shroomery.org/forums/showflat.php/Number/11179100


Edited by nightflyer (09/06/09 08:19 AM)


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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: nightflyer]
    #11004939 - 09/05/09 05:34 PM (12 years, 11 months ago)

:scaryshroom::thumbup::thanx:


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InvisibleElSeta
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11005938 - 09/05/09 10:14 PM (12 years, 11 months ago)

What if you inoculate grain spawn directly to fresh cut grass and let it in open air.
_____________________________________________________________________
I'm looking for a semilanceata print, please PM me.


--------------------
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Looking for Lion's Mane and Yellow Oyster culture. Will trade for: Shiitake/King Oyster/Blue Oyster/White Beech.
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: ElSeta]
    #11006033 - 09/05/09 10:33 PM (12 years, 11 months ago)

I tried a 1 quart of LC once, on some nice thick lush well manicured grass.

Nothing... Although there still might be a chance, but I doubt it.


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: mushroomhunter10]
    #11041744 - 09/11/09 06:58 PM (12 years, 10 months ago)

Got my supplies 2day!:thumbup:  got some cleaning then getting organized to do then I'll get the glove box/pc out and start my agar work.

so how long b4 I should start making spawn jars?
:scaryshroom::thumbup:


13:mushroom2:


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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11041866 - 09/11/09 07:28 PM (12 years, 10 months ago)

After you have isolates on agar.


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Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: mushroomhunter10]
    #11042517 - 09/11/09 09:44 PM (12 years, 10 months ago)

Quote:

mushroomhunter10 said:
After you have isolates on agar.




arent we Captain Obvious:tongue2:

I meant....how long ONCE I GET ISOLATES....I have to let the myc grow out a lil b4 I can use it for inoculations.  so like a 2" in diameter or like 3 days is more of what Im looking for.  is there a best time to use it or what?:shrug:

on another note::sun:
anybody else purchase an agar kit from sporeworks b4, I was just wondering if the flask that comes with it is PC-able or not?


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Edited by 13shrooms (09/27/09 02:18 AM)


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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11042794 - 09/11/09 10:38 PM (12 years, 10 months ago)

does it say pyrex on it anywhere?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: ScavengerType]
    #11042839 - 09/11/09 10:44 PM (12 years, 10 months ago)

nope, just the brand Nalgene 500ml bottom has a mark with P.M.P. and the lid is the same.:shrug:

nevermind.  I found the answer on sporeworks site.  Its autoclavable. :smile:


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Edited by 13shrooms (09/27/09 02:20 AM)


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Invisible13shroomsM
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Re: "Indoor Liberty Caps Community Tek" (work in progress) [Re: 13shrooms]
    #11134940 - 09/27/09 02:37 AM (12 years, 10 months ago)

Alright,  I currently am not able to do any agar work anywhere in my house due to massive amounts of mold growing in my kitchen (walls in and out) and 2 side porches.  all severly water damaged and completely engulfed in mold and random fungi.My kitchen ceiling caved from the flat roof above it holding water (well not any more). 
so untill I get a new roof, kitchen, porches and remove/destroy all the mold I can I wont be bustin out the agar projects. :frown:

feel free to continue adding info/suggestions here.


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