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OfflineBob_J
Spaced outRabbit

Registered: 12/30/01
Posts: 246
Loc: alberta canada
Last seen: 7 years, 7 days
cloning in pF jars?
    #1097130 - 11/29/02 11:50 PM (18 years, 8 months ago)

hi, ive been growing mushrooms for about 2 years for personal use. ive stuck to the PF tek as its easy to do and i dont need lots.
i want to try and clone my shrooms. i read paul staments book a few years back, but unfortunitaly it went missing this year so i cant read up on it. i believe it had something about cloning, i know it has to be sterile in a glovebox and you slice the shroom down the middle and take out the inside of it? right?
heres where i get lost. since im growing PF style, how would i get the piece of stem inside the jars,? ive also read that you can use a syringe to do it to, but i dont know the tek or how it would work. ive searched the FAQ's maybe im a idiot..cant find much info on cloning.
can anybody give me a link or any simple advice on this.

thanks
bob j


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"With insomnia your never really asleep, and your never really awake"


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Offlinerhizo
herb eater
Registered: 10/31/02
Posts: 599
Loc: Superposition of possible...
Last seen: 2 years, 10 months
Re: cloning in pF jars? [Re: Bob_J]
    #1097237 - 11/30/02 12:20 AM (18 years, 8 months ago)

you could go about it a few ways. i think agar would be the best and most interesting, but if you want simple and easy then i would suggest using the syringe method. the basic idea is to get a nice sterile piece of healthy myc from the stem or cap and then transfer it to agar or put it in a sterile solution of water and grind it into tiny pieces for inoculation. i've used a coffee grinder type of thing(a blender with a seed miller attachment) with good success, but i guess you can try pieces of glass or something else to break it up. here's a link
http://www.mycotopia.net/discus/messages/5/3384.html?1004627146


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An optimist is never pleasantly surprised.


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Anonymous

Re: cloning in pF jars? [Re: Bob_J]
    #1097515 - 11/30/02 02:19 AM (18 years, 8 months ago)

You can use a chunk of the inside of the stem and grow karo/honey water. Just look for karo water on the message board or in the FAQ.


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