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hi, ive been growing mushrooms for about 2 years for personal use. ive stuck to the PF tek as its easy to do and i dont need lots. i want to try and clone my shrooms. i read paul staments book a few years back, but unfortunitaly it went missing this year so i cant read up on it. i believe it had something about cloning, i know it has to be sterile in a glovebox and you slice the shroom down the middle and take out the inside of it? right? heres where i get lost. since im growing PF style, how would i get the piece of stem inside the jars,? ive also read that you can use a syringe to do it to, but i dont know the tek or how it would work. ive searched the FAQ's maybe im a idiot..cant find much info on cloning. can anybody give me a link or any simple advice on this.
thanks bob j
-------------------- "With insomnia your never really asleep, and your never really awake"
you could go about it a few ways. i think agar would be the best and most interesting, but if you want simple and easy then i would suggest using the syringe method. the basic idea is to get a nice sterile piece of healthy myc from the stem or cap and then transfer it to agar or put it in a sterile solution of water and grind it into tiny pieces for inoculation. i've used a coffee grinder type of thing(a blender with a seed miller attachment) with good success, but i guess you can try pieces of glass or something else to break it up. here's a link http://www.mycotopia.net/discus/messages/5/3384.html?1004627146
-------------------- An optimist is never pleasantly surprised.
Re: cloning in pF jars? [Re: Bob_J] #1097515 - 11/30/02 02:19 AM (14 years, 8 months ago)
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