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captainm424
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Registered: 08/01/09
Posts: 145
Last seen: 13 years, 3 months
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Sad day 24 jars with Penicillium spp. *DELETED*
#10959259 - 08/29/09 12:40 AM (14 years, 6 months ago) |
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Post deleted by captainm424Reason for deletion: 1
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DWGERRISH
Stranger (than you)



Registered: 07/20/09
Posts: 79
Loc: plantasia
Last seen: 9 years, 3 months
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Re: Sad day 24 jars with Penicillium spp. [Re: captainm424]
#10959261 - 08/29/09 12:41 AM (14 years, 6 months ago) |
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damn that bites
-------------------- Everything contained in my posts are the expected results i have come to anticipate from knowledge obtained through the reading of information published for the general public. All scenarios are strictly hypothetical and there is no true research that i have done personally to support any statements that i have made. Watch this vid What to do during police encounters
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kaste
six-nine power slider



Registered: 12/29/08
Posts: 2,401
Loc: Latvia
Last seen: 6 years, 4 months
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Re: Sad day 24 jars with Penicillium spp. [Re: DWGERRISH]
#10959269 - 08/29/09 12:44 AM (14 years, 6 months ago) |
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Sucks. Better luck nxt time.
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  It could be worse. A woman could cut off your penis while you're sleeping and toss it out the window of a moving car.
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Alabama Slim
Chinese river witch

Registered: 06/29/08
Posts: 7,439
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Edited by Alabama Slim (02/02/12 10:51 AM)
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captainm424
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Registered: 08/01/09
Posts: 145
Last seen: 13 years, 3 months
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Re: Sad day 24 jars with Penicillium spp. *DELETED* [Re: Alabama Slim]
#10959359 - 08/29/09 01:08 AM (14 years, 6 months ago) |
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Post deleted by captainm424Reason for deletion: 1
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Alabama Slim
Chinese river witch

Registered: 06/29/08
Posts: 7,439
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Edited by Alabama Slim (02/02/12 10:51 AM)
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chronosync
kicked the habit. shed my skin.

Registered: 01/23/09
Posts: 692
Last seen: 8 years, 3 months
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Re: Sad day 24 jars with Penicillium spp. [Re: Alabama Slim]
#10959660 - 08/29/09 02:28 AM (14 years, 6 months ago) |
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if you PC properly, have the right kind of lid, are very careful with your procedures, eliminate drafts, use latex gloves and possibly a face mask, wipe everything down with alcohol, flame sterilize your needle, cool it with an alcohol soaked towel immediately prior to inoculation, open air inoculations are possible. although more risky than using a glove box/still air chamber. (you actually dont need any gloves atatched to the box, just latex gloves on your hands, if thats your preference)
i just use a glovebox, or what i prefer to call a "still air chamber", to be safer. its worth it. even just for peace of mind. way less risky. it also adds a control step and eliminates one of the many vectors for contamination.
>>>condoms arent always necessary... but why not use one... just in case.
also. make sure to eliminate all drafts in your work area. as much as possible. close all windows. turn off EVERY fan. etc. even when working with a glovebox.
it helps if the inside of the glovebox/still air chamber is either misted down slightly with everything loaded in first, then covered. (i use a %10 bleach solution). or wiped down with soapy water and then loaded. the damp surfaces tend to trap anything that might be floating around.
get a large clear sterilite container. make two holes for your arms.
it will be sort of like your new mini-lab.
-edit-
and i forgot to say...
GOOD LUCK!
Edited by chronosync (08/29/09 02:30 AM)
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