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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Something earned that might be helpful f/ newbies
    #1094975 - 11/29/02 01:18 AM (14 years, 7 months ago)

Hey ive been lurking in the forums since july but this is my first post ever, I have spent many nights reading my ass off, anyways i went thru a whole suringe and just turned out with contams, finaly ive had success with the changing of one variable , i figured my problem, it was at the time of incolation, THERE IS NOT ENOUGH EMPHASIS IN ANY OF THE TEKS PUT ON THE POINT OF INCOLATION, they dont realy say how imporant it is to squirt the sporewater down the edge of the jar, yes they do say it, but when i did it i was so afaid of contams from everyone on here that i incolated very speedily straight down the middle and didnt pay attention to where it was squirting, IT IS VERY IMPORANT THAT U SEE THE SPOREWATE DRIP DOWN THE SIDE OF THE JAR, dont get scared like me and hurry up and stick yer needle in yer lil hole straight in the center, ever since i changes this one part of my process, i have had much success, THANKS EVERYONE!!!!!!!!!!!!!!!!!!!!!!!!!!!!!


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NONE OF THE ABOVE IS TRUE I LIKE TO WRITE STORIES


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Something earned that might be helpful f/ newbies [Re: deprave]
    #1095254 - 11/29/02 02:55 AM (14 years, 7 months ago)

There isn't much of a difference if you innoculate through center or sides except for a colonization time difference. But if it works for you then go ahead.


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
Posts: 24,123
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Last seen: 29 days, 11 hours
Re: Something earned that might be helpful f/ newbies [Re: deprave]
    #1095291 - 11/29/02 03:04 AM (14 years, 7 months ago)

>they dont realy say how imporant it is to squirt the sporewater down the edge of the jar, yes they do say it

So what, do they say it or not? :wink: 


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Something earned that might be helpful f/ newbies [Re: deprave]
    #1095323 - 11/29/02 03:10 AM (14 years, 7 months ago)

I recommend inoculating in the centre AND the sides of the jar which I think speeds up colonization even more.

Just my opinion


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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (11/29/02 03:11 AM)


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Something earned that might be helpful f/ newbies [Re: Bi0TeK]
    #1095350 - 11/29/02 03:19 AM (14 years, 7 months ago)

Yeah me as well. A fifth innoculation point to the center speeds things up a bit.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: Something earned that might be helpful f/ newbies [Re: Anno]
    #1095632 - 11/29/02 10:36 AM (14 years, 7 months ago)

LoL, Sorry bout the hiddeous typing there, i was a bit intoxicated lastnite when i posted, all i realy ment to say was that THERE IS NOT ENOUGH EMPHASIS PUT ON THAT STEP IN MANY TEKS, im not badmouthing any Tek writers im just saying that there is not enough emphasis usually put on the importance of this from my interpratation which caused me to waste a syringe, Just trying to help  :grin: 


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NONE OF THE ABOVE IS TRUE I LIKE TO WRITE STORIES


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OfflineShaw
hick

Registered: 06/28/01
Posts: 2,218
Loc: midwest, usa
Last seen: 5 months, 12 days
Re: Something earned that might be helpful f/ newbies [Re: deprave]
    #1096029 - 11/29/02 02:33 PM (14 years, 7 months ago)

i really don't see how the other way causes contams


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OfflineFred Garvin
Male Prostitute
Registered: 09/24/02
Posts: 1,657
Loc: The northern part of sout...
Last seen: 10 years, 6 months
Re: Something earned that might be helpful f/ newbies [Re: deprave]
    #1096052 - 11/29/02 02:44 PM (14 years, 7 months ago)

I believe that you just had a contaminated syringe, or you did something else wrong. Inoculating on the side or in the middle makes no difference in regards to contamination.



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The above statements are just the incoherent babblings of your friendly neighborhood Cracker!

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