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OfflineTheManWithTheHat
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Chimeras
    #10937380 - 08/25/09 08:40 PM (14 years, 5 months ago)

Does anyone have procedural information on how these are generally created in the lab?
I might have access to the facilities to do some work on campus in the fall and I was wondering if anyone could shed some light or offer some useful information

I think I plant to try to created some cactus chimeras--and of course if I am successful I shall share with my fellow shroomerites :thumbup:

-tmwth


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OfflineCactusdan
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Re: Chimeras [Re: TheManWithTheHat]
    #10937414 - 08/25/09 08:44 PM (14 years, 5 months ago)

Wiki article on chimeras for those who don't know:
Quote:

Typically seen in non-human zoology (but also discovered to a rare extent in human beings), a chimera is an animal that has two or more different populations of genetically distinct cells that originated in different zygotes; if the different cells emerged from the same zygote, it is called a mosaicism.

Chimeras are formed from four parent cells (two fertilized eggs or early embryos fused together) or from three parent cells (a fertilized egg is fused with an unfertilized egg or a fertilized egg is fused with an extra sperm). Each population of cells keeps its own character and the resulting animal is a mixture of tissues.

This condition is either inherited, or it is acquired through the infusion of allogeneic hematopoietic cells during transplantation or transfusion. In nonidentical twins, chimerism occurs by means of blood-vessel anastomoses. The likelihood of a child being a chimera is increased if the child is created via in vitro fertilization. Chimeras can often breed, but the fertility and type of offspring depends on which cell line gave rise to the ovaries or testes; intersexuality and hermaphroditism may result if one set of cells is genetically female and another genetically male.





Now to answer your question I think it's usually done through genetic engineering almost.

I'd look at this line of the article posted:

"or it is acquired through the infusion of allogeneic hematopoietic cells during transplantation or transfusion."

That's about as much as I could help.

I'd wait for Methadone on this one though, I know he's done far more research into this than I have.


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InvisibleMethadone
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Re: Chimeras [Re: Cactusdan]
    #10937902 - 08/25/09 09:55 PM (14 years, 5 months ago)

This link is something ive had saved for awhile.. Should answer LOTS of your questions: http://aggie-horticulture.tamu.edu/tisscult/chimeras/chimera.html


It seems via tissue culture that chimeras cant be created, most of the work done is done to maintain the chimeral tissue (preventing reversion) during introduction to the sterile culture environment. Im not sure if a plant can be 'forced' into mutation, besides polyploidy (do a search, someone did an excellent wrietup on it somewhere on the S, my apologies if they read this). My elementary understanding of polyploidy is that you're adding an extra chromosome to the original plant to make it 'more' of what it originally was. I *assume* More potent, faster, stronger, etc..

This isnt anything I have as much an understanding about as id like to, but I can say for certain that if anybody has figured out how to make the types of chimeras were familiar with, they sure arent sharing it. It seems the only way to acquire them is chance.. via grafting. After the fact, they can be maintained as chimeras via micropropagation with some finesse, but NOT created.

Thats my understanding of it anyways, hope the link helps! If you get to playing in the lab, show us some pics.

-M


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OfflineTheManWithTheHat
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Re: Chimeras [Re: Methadone]
    #10937974 - 08/25/09 10:07 PM (14 years, 5 months ago)

Quote:

Methadone said:
This link is something ive had saved for awhile.. Should answer LOTS of your questions: http://aggie-horticulture.tamu.edu/tisscult/chimeras/chimera.html


It seems via tissue culture that chimeras cant be created, most of the work done is done to maintain the chimeral tissue (preventing reversion) during introduction to the sterile culture environment. Im not sure if a plant can be 'forced' into mutation, besides polyploidy (do a search, someone did an excellent wrietup on it somewhere on the S, my apologies if they read this). My elementary understanding of polyploidy is that you're adding an extra chromosome to the original plant to make it 'more' of what it originally was. I *assume* More potent, faster, stronger, etc..

This isnt anything I have as much an understanding about as id like to, but I can say for certain that if anybody has figured out how to make the types of chimeras were familiar with, they sure arent sharing it. It seems the only way to acquire them is chance.. via grafting. After the fact, they can be maintained as chimeras via micropropagation with some finesse, but NOT created.

Thats my understanding of it anyways, hope the link helps! If you get to playing in the lab, show us some pics.

-M



Thanks dude, I'll start reading over it
I have seen the thread on inducing polyploidy too, though it was a while ago


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InvisibleMethadone
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Re: Chimeras [Re: TheManWithTheHat]
    #10938353 - 08/25/09 11:04 PM (14 years, 5 months ago)

No problem, chimeras are extremely interesting, nothing in this world piques my curiosity as much as a true chimera. Other recommended reading about Chimera's: Wikipedia "Chimera", The "Bizzaria" Orange (Darwin did some writing about it if I'm not mistaken, its an old orange/citron chimera which originated in France if memory serves), Calico cats, and stem cell research (using animal eggs w/ human sperm).

Very advanced and interesting area of science, im going to PM you a book I recommend.

-M


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OfflineHorticulture
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Re: Chimeras [Re: Methadone]
    #10938504 - 08/25/09 11:22 PM (14 years, 5 months ago)

I know it has a tendency to happen with some fruit tree grafts.  It's pretty fascinating stuff, but I haven't looked much into it.


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OfflineTheManWithTheHat
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Re: Chimeras [Re: Horticulture]
    #10938814 - 08/26/09 12:17 AM (14 years, 5 months ago)

Quote:

its hypothesized that some monstrous/chimeras originate from bacterial, fungal, and viral infection



I personally think then it would be really interesting to see if you could locate any foreign organisms on a cristate/etc cactus that weren't also in a normal cactus grown in the same environment under a high powered microscope or something
And then try to culture whatever is causing the growth, oh gosh :boobs:


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Offlinekadakuda
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Re: Chimeras [Re: TheManWithTheHat]
    #10941307 - 08/26/09 12:25 PM (14 years, 5 months ago)

i think the main way of getting them is grafting (with cacti).  i have not seen any really successful intentional attempts.  but some species seem a little more prone that others....still a long shot though.

do pass on something if ya get a clue though :smile:


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OfflineTheManWithTheHat
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Re: Chimeras [Re: kadakuda]
    #10943672 - 08/26/09 06:30 PM (14 years, 5 months ago)

Quote:

kadakuda said:
i think the main way of getting them is grafting (with cacti).  i have not seen any really successful intentional attempts.  but some species seem a little more prone that others....still a long shot though.

do pass on something if ya get a clue though :smile:



If I do so, I shall let you know the moment after I publish it--because that's some really intense stuff right there

If by some freak chance I do though, you shall all receive some inside informayshun


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