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Offlineresin
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Kero tek.
    #1094185 - 11/28/02 04:59 PM (14 years, 1 month ago)

Could somebody please provide me with a tek using karo to make liquid mycellium? Can I do about 10 jars and pick the best kinda ike a pure isolate?


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Offlineribbit
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Re: Kero tek. [Re: resin]
    #1094723 - 11/28/02 10:54 PM (14 years, 1 month ago)

for 1. its karo.

and

1 tspn of karo per 100 mL distilled/sterile water

30 min @ 15 psi.

even better:

1 tspn dextrose per 100 mL distilled/sterile water

30 min @ 15 psi.


get dextrose here -> brewery

-need to make an air exchange. can use polyfill or a filterdisk

-should put some shards of glass to break up the mycelium when needed.

-shake the jar every other day for a week

-keep the jar in your incubation chamber for 1 week

-when you get your results, you can store in the fridge for up to about 2 months

**be sterile** this is an easy invite for contam


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OfflineBaby_Hitler
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Re: Kero tek. [Re: ribbit]
    #1094761 - 11/28/02 11:22 PM (14 years, 1 month ago)

You can buy fructose at Wal-Mart. It is also a monoglyceride, do you think it would work as well as dextrose?


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Offlinehappygrins
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Re: Kero tek. [Re: ribbit]
    #1094765 - 11/28/02 11:27 PM (14 years, 1 month ago)

On my friends personal experiences with karo he's used:

100 ml water
1/2 Tspn Light Karo syrup

PC'ed for 15 minutes..  He found that if he used the whole Tspn of Karo that his Karo would tend to carmelize thus making it harder to spot contams. 

  >Can I do about 10 jars and pick the best kinda ike a pure isolate?

Im sure you could do this, or even try doing a Stem Cell transfer into the Karo Water..  This works great.

--happygrins :grin:


Edited by happygrins (11/28/02 11:30 PM)


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Invisiblezeta
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Re: Kero tek. [Re: Baby_Hitler]
    #1095296 - 11/29/02 03:05 AM (14 years, 1 month ago)

Rember, dextrose is just another name for glucose.
If they have fructose then they definitely have glucose. Just get the glucose


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OfflineBaby_Hitler
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Re: Kero tek. [Re: zeta]
    #1095400 - 11/29/02 03:49 AM (14 years, 1 month ago)

No, they definitely don't have Glucose/Dextrose just "Sugar" and Fructose.


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