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InvisibleJ3illy
Trainee

Registered: 10/18/08
Posts: 3,344
Re: First time grower... used dried shroom gills bought from dealer [Re: JamesChappy]
    #9402564 - 12/09/08 08:59 PM (15 years, 2 months ago)

Ouch that sucks bro.  That sounds like trich, so hopefully you didn't get any of the spores onto the prints you took.  If everything doesn't work out OK tho, based on this extremely dedicated effort you made - I'm sure someone would hook you up w/ a print or something.  Good luck man.


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Invisiblecreekfreek
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Registered: 01/26/08
Posts: 4,818
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Re: First time grower... used dried shroom gills bought from dealer [Re: J3illy]
    #9408472 - 12/10/08 06:53 PM (15 years, 2 months ago)

Quote:

J3illy said:
Ouch that sucks bro.  That sounds like trich, so hopefully you didn't get any of the spores onto the prints you took.  If everything doesn't work out OK tho, based on this extremely dedicated effort you made - I'm sure someone would hook you up w/ a print or something.  Good luck man.





After all the work you went thru on this project, if your print is bad, I will hook you up with a good one.


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OfflineJamesChappy
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Re: First time grower... used dried shroom gills bought from dealer [Re: creekfreek]
    #9409089 - 12/10/08 08:15 PM (15 years, 2 months ago)

wow, thanks all. I'm semi confident in my print. I didn't see any green until just a few days ago and I did the prints about a week, week and a half before that. I hoped at first to combat the trich because it was in a nice contained section. I cut out the infected part and took out a good inch of "good" cake with it. I heated the knife before each cut I made to continue to kill off whatever got onto the blade. Two days later more green bubbles. Oh well. I got two harvests out of a pretty ghetto grow method. I'll start my next grow after Christmas I think. If it's got contams I'll prolly hit you guys up for some prints : :thanx:


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Offlinetr8orjohn
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Re: First time grower... used dried shroom gills bought from dealer [Re: JamesChappy]
    #9409694 - 12/10/08 10:14 PM (15 years, 2 months ago)

Just finished reading this thread...good job, even though it ended in heartbreak(ish)

To tally it up: old, dried up source, unsterilized transfer (the turkey baster...), suffocating the cake, using vermiculite instead of perlite, and low temperatures...and it *still* worked!

The mushroom g-ds have smiled upon you...and you give me hope in being able to do some of this neat stuff myself, as all this talk of contam from other folks got me scared....

Either way, good job man!

jon


--------------------
--------------------------------------------------------------------------------------------------------

Microbiologist turned mycologist, but still amateur enough to enjoy the occasional surprise...

My Trade List

Terry M said:
Looking for rules that other people use successfully. Sterile procedure is just a set of rules that work.


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OfflineJamesChappy
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Re: First time grower... used dried shroom gills bought from dealer [Re: tr8orjohn]
    #9416542 - 12/12/08 04:10 AM (15 years, 2 months ago)

hey, I boiled the shit outta that turkey baster before I used it. The transfer wasn't ideal because instead of doing a controlled injection with little to no chance of airborn spores landing in my substrate, I had to open the lids of my jars and pour in the spore solution in open air. The whole suffocating the cake really wasn't too bad, considering that the cake had fully colonized, but only just that day. Many guides suggest giving the cake a few days after colonization to birth so the mycellium can continue to fill out. I do blame the trich on the vermiculite. I thought I smelled some kind of mustyness a few days before I saw any green, and it was coming from the verm, not the cake. The vermiculite actually did a fairly good job of humidifying the container. I should have sterilized it first though. There's a twofold advantage to perlite. It has more surface area to expose water, and it isn't as prone to harboring contams. Oh, also the low temps were only throughout the colonization period and early in the birthing period. I blame the slow growth during that time on the temps. Just a few days after birthing I put the container on top my laptop, which slightly warmed the verm, which created more humidity and brought the temp inside the chamber to optimal growth temps.

All in all... I did get very lucky, again and again. I had a feeling when I started this little project that growing mushrooms wasn't as hard as it is made out to be. I think if you follow the guides to the letter and are very anal about sterility you'll increase your odds for success to near 100%. I think I had a total of 8 jars I've tried to grow. Only one had success. I guess the lesson is, the closer you follow the guides, the less time and materials you'll waste of failed jars.

If someone asked me how to grow mushrooms from dried shroom gills, I'd tell them to go out and buy everything recommended in a pftek, along with some agar. I would definitely not recommend doing it the ghetto method I tried. I did get lucky. I think I basically ended up making chocolate chip cookies by looking at a recipe, substituting half the ingredients with whatever I had in the kitchen, then asking real bakers for advice. Yeah, I got hella lucky.


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OfflineThe Centre
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Registered: 10/04/08
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Re: First time grower... used dried shroom gills bought from dealer [Re: veda_sticks]
    #9416636 - 12/12/08 05:10 AM (15 years, 2 months ago)

Quote:

veda_sticks said:
the problem with using mushrooms as inoculant is that they have been exposed to the elements and are likely to be covered in contaminants. Your shrooms have been god knows where and are likely heavily contaminated. Which you are experiencing with the pf tek.

You cant transfer mycelium from that jar, it will most likely result in more contamination.

Your best option is to start growth using agar on petri dishes and transfer mycelium furthest away from the contamination. Repeat these transfers until you have a clean sample.

It would be much simpler and easier to just spend $18 dollers on a spore syringe rather than wasting substrate and materials trying to clone a dried mushroom.

Your substrate looks heavily colinised with something else, bacteria or mould. Toss it.




Or $5: Check the deals at magicmushrooms.org


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Invisibleseven
.
Male

Registered: 06/09/08
Posts: 1,478
Loc: north carolina
Re: First time grower... used dried shroom gills bought from dealer [Re: The Centre]
    #9416712 - 12/12/08 06:04 AM (15 years, 2 months ago)

Quote:

The Centre said:
Quote:

veda_sticks said:
the problem with using mushrooms as inoculant is that they have been exposed to the elements and are likely to be covered in contaminants. Your shrooms have been god knows where and are likely heavily contaminated. Which you are experiencing with the pf tek.

You cant transfer mycelium from that jar, it will most likely result in more contamination.

Your best option is to start growth using agar on petri dishes and transfer mycelium furthest away from the contamination. Repeat these transfers until you have a clean sample.

It would be much simpler and easier to just spend $18 dollers on a spore syringe rather than wasting substrate and materials trying to clone a dried mushroom.

Your substrate looks heavily colinised with something else, bacteria or mould. Toss it.




Or $5: Check the deals at magicmushrooms.org


-USE A SHROOMERY SPONSOR!


--------------------
grind


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OfflineThe Centre
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Re: First time grower... used dried shroom gills bought from dealer [Re: German Kahuna]
    #9416717 - 12/12/08 06:06 AM (15 years, 2 months ago)

Quote:

German Kahuna said:
Quote:

4 ho cultivar said: and that bubble is weird, i thought that this whole time..... what the bugger is that fucker




I'd say it's a mutant. I'd eat it. :crazy2:




Isn't it maby a truffle?


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OfflineThe Centre
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Re: First time grower... used dried shroom gills bought from dealer [Re: Michael O G]
    #9416720 - 12/12/08 06:08 AM (15 years, 2 months ago)

Quote:

Michael O G said:
I'm glad I'm not the only one who started like this.  This thread has inspired me to finally make an account after two and a half years of lurking.  Shh, the good old days of baggy dust and eye droppers.  If you have the determination to make this work, it only gets easier.  Paranoia is probably the best thing you can have with a hobby like this.  Enjoy the anonymity.  I started with the furriest slowest strain I've ever heard of.  Today, I got a myself a few Lc's of some good quick Rhizo's.  It's all just a matter of patience, willingness to learn, and this site!

OT: 'ello all! (First post)




Or the worst. Bad trips for all!


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OfflineThe Centre
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Re: First time grower... used dried shroom gills bought from dealer [Re: seven]
    #9416739 - 12/12/08 06:25 AM (15 years, 2 months ago)

Quote:

seven said:
Quote:

The Centre said:
Quote:

veda_sticks said:
the problem with using mushrooms as inoculant is that they have been exposed to the elements and are likely to be covered in contaminants. Your shrooms have been god knows where and are likely heavily contaminated. Which you are experiencing with the pf tek.

You cant transfer mycelium from that jar, it will most likely result in more contamination.

Your best option is to start growth using agar on petri dishes and transfer mycelium furthest away from the contamination. Repeat these transfers until you have a clean sample.

It would be much simpler and easier to just spend $18 dollers on a spore syringe rather than wasting substrate and materials trying to clone a dried mushroom.

Your substrate looks heavily colinised with something else, bacteria or mould. Toss it.




Or $5: Check the deals at magicmushrooms.org


-USE A SHROOMERY SPONSOR!




They are.


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Offline4 ho cultivar
Transcending plains
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Re: First time grower... used dried shroom gills bought from dealer [Re: The Centre]
    #9435073 - 12/15/08 05:03 AM (15 years, 2 months ago)

good job chappy. Just keep plugging away like the little engine that could....
I'm so impressed. how are the potency of those buggers????? I like everthing but
more picks though. everyone loves pictures. pictures of your dog we don't care. just not your butt. but maybe your girlfriend butt. that would be o.k. :spank:


--------------------
:mushroom2::crazy2::mushroom2::crazy2::Patience be thy virtue:crazy2::mushroom2::crazy2::mushroom2:

Blurring the line between reality and dreams
Mr. Winkie says: "Procrastinators unite................................tommorrow!!:ganja:
G.Kahuna said: "Plus you get all the fun from taking a walk {and} shooting mycelium at cow droppings with a super soaker. Now beat that!":cuteshit:


Edited by 4 ho cultivar (12/15/08 05:04 AM)


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Offlineveda_sticks
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Re: First time grower... used dried shroom gills bought from dealer [Re: 4 ho cultivar]
    #9435441 - 12/15/08 08:34 AM (15 years, 2 months ago)

I take back my last previous statement about not being able to use mycelium from contaminated jars. Not only have you proven that it can be done.

I have seen a few others do it.

A mate of mine has done it too. too bad its liberty caps though which are almost imposible to fruit LOL

TheCentre, the OP didnt want to buy spores as to have no trail. Overkill in my opinion though but thats the route he wanted to go.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinekingjames488
Stranger
Registered: 02/18/09
Posts: 435
Last seen: 12 years, 8 months
Re: First time grower... used dried shroom gills bought from dealer [Re: JamesChappy]
    #10196319 - 04/19/09 04:08 PM (14 years, 9 months ago)

im not sure if this has been mentioned, as i didnt want to read all 7 pages of the thread or w.e.

but if you made like mini cakes it might work out.

just like about 1/2" tall and 1" around or something.

they would colonize faster, and prolly give less chance for contams to fight the myc. growth.

then should take less time from inoculation to fruiting and maybe you could get a shroom to take a SMALL print from, but at least its something.


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OfflineJamesChappy
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Re: First time grower... used dried shroom gills bought from dealer [Re: kingjames488]
    #10819787 - 08/07/09 07:57 PM (14 years, 6 months ago)

Well, a little update for anyone who read this thread... almost a year ago. I finally got around to trying the shrooms that were succesfully produced. After it was all said and done, I lost most of the shrooms to improper drying procedures. I put most of them in an airtight container with what I was told was dissicant. It was not, the mushrooms got mouldy and had to be thrown out. I was left with about 1.5 grams of good dried shrooms. Three days ago they were enough to get a buddy and I lightly buzzed. Now I'm starting up another grow using the spore print I collected from the first flush. I've never used syringes before, but I sucked it up and bought some from the drug store and I picked up some good jars and a pressure cooker. Time to do this right...


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OfflineP.Menace
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Re: First time grower... used dried shroom gills bought from dealer [Re: JamesChappy]
    #10819810 - 08/07/09 08:05 PM (14 years, 6 months ago)

desiccant removes water from the mushrooms....not the container... nor does it keep the water out... when packed all together like that over time everything inside said container would eventually even out to an average humidity.
if you left it all together without making sure that they were already 100% cracker dry then you simply stored them for failure as you have seen now


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.


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OfflineJamesChappy
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Re: First time grower... used dried shroom gills bought from dealer [Re: P.Menace]
    #10895455 - 08/20/09 03:46 AM (14 years, 5 months ago)

Good news, of the three jars that I inoculated, one took. It appears to be healthy mycelium so far. Unfortunately ants have invaded my place, so I'm trying to find a good way of keeping them out of the jar (right now the top is covered in aluminum foil and I've sealed the jar in a zip lock for extra ant protection...

Hopefully the whole jar will grow out successfully and I'll take some myc from that and spread it into another empty jar. We'll see... I'm just happy that something took... I hadn't exactly been kind to my spore print...


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OfflineLostkeys
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Re: First time grower... used dried shroom gills bought from dealer [Re: JamesChappy]
    #10895557 - 08/20/09 04:45 AM (14 years, 5 months ago)

Quote:

JamesChappy said:
Good news, of the three jars that I inoculated, one took. It appears to be healthy mycelium so far. Unfortunately ants have invaded my place, so I'm trying to find a good way of keeping them out of the jar (right now the top is covered in aluminum foil and I've sealed the jar in a zip lock for extra ant protection...

Hopefully the whole jar will grow out successfully and I'll take some myc from that and spread it into another empty jar. We'll see... I'm just happy that something took... I hadn't exactly been kind to my spore print...




If the ants are "sweet ants" look for some stuff called "Terro" at your local hardware or home improvement store.  It comes as a little bottle and some squares of cardboard that you cut out yourself.  Place a drop on each piece of cardboard and place them where the ants can find them.

I don't know what's in it but I've killed entire ant colonies with the stuff... they literally crawl over top of each other and I've seen them cross a whole room to get to it, climbing over the dead body's of their brethren to get to the precious poison.  If they survive collecting the goop, they take it back to their lair and feed it to the queen.

It's awesome if you're into the wholesale slaughter of an entire colony.  I'm not so violent towards nature these days.


--------------------
So heavy I fell through the earth...



Edited by Lostkeys (08/20/09 04:47 AM)


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Offlineveda_sticks
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Re: First time grower... used dried shroom gills bought from dealer [Re: Lostkeys]
    #10895840 - 08/20/09 07:14 AM (14 years, 5 months ago)

cover the hwole sin your jars with micrpore tape, that should stop any bugs getting in.

keep an eye out for compititions also, there have been quite a few recently, there would have been 2 more but they go ruined.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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