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Offlineupupup
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Problems getting Rhizo growth from MEA
    #1088785 - 11/26/02 10:27 PM (14 years, 10 days ago)

In a future life I am having a hard time getting Rhizomorphic growth to happen out of my Multi Spore Innoculations onto MEA from Myco Supply. My psycic tells me that I am only seeing that Intermediate linear type. The crystal ball says I tried it with spores from a syringe and from print scrapings.

I think at some point I may wonder if the strain has anything to do with it? Psilo. Cambo's. Being new to the whole clarivoyant idea and to a future of MEA I wonder what I might do to encourage rhyzo growth?

How many transfers might I have to do to get to the point of that pic on the cover of TMC? Should I use peroxided MEA or just straight MEA in the future?


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Support bacteria - they're the only culture some people have.


Edited by upupup (11/26/02 11:03 PM)


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InvisibleJoshua
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Registered: 10/27/98
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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1088881 - 11/26/02 10:56 PM (14 years, 10 days ago)

After one to two transfers you should start seeing rhizomorphic growth. I am using MEA, I have some strains that grew rhizos on the first isolation, others took two isolations.

Joshua


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OfflineOn_the_Down-Low
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Re: Problems getting Rhizo growth from MEA [Re: Joshua]
    #1089215 - 11/27/02 12:21 AM (14 years, 10 days ago)

Dumb question #116: Is there a connection between the agar and the lack of rhizomorphic growth? I thought it would be like the jars, some good rhizome growth and some not as good. No?


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Offlineupupup
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Re: Problems getting Rhizo growth from MEA [Re: On_the_Down-Low]
    #1090578 - 11/27/02 11:08 AM (14 years, 9 days ago)

Wondering that same question especially after reading in TMC where they talk about the different agar recipies and them having favorites, the one out of the three they mentioned as NOT being a favorite was MEA. THey also mentioned that rye flour added to the agar helped with rhizo growth.

Anybody try this?


--------------------
Support bacteria - they're the only culture some people have.


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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1091868 - 11/27/02 08:36 PM (14 years, 9 days ago)

I find that the addition of a bit of yeast helps produce more rhizomorphic mycelium. But if transferring a linear isolation to MYA makes it more rhizomorphic it won't change the fruiting characteristics of the isolation....


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hmmm........


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Offlineupupup
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Re: Problems getting Rhizo growth from MEA [Re: Champion des Champignons]
    #1092644 - 11/28/02 01:02 AM (14 years, 9 days ago)

I really want to be smart...really, but what the hell did you say?

If I got that right your saying I might fidget with the "conditions" but it won't matter if the stuff dosen't have the umph to begin with?


--------------------
Support bacteria - they're the only culture some people have.


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InvisibleChampion des Champignons
long standing member;)

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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1092924 - 11/28/02 02:41 AM (14 years, 9 days ago)

what I was getting at is....
well, I mostly deal with clones on agar, rather than mutispores y'see. In my experience a cloned culture produces more rhizomorphic mycelium on MYA than MEA.
If you were to isolate a pure linear culture from a multispore inoc. which didn't produce rhizomorphic mycelium on MEA and then transferred it to MYA where it suddenly did, this wouldn't indicate any change in the genetics of the isolation, ie it would fruit the same whether or not it was growing rhizomorphically on agar or not...kinda thing:tongue:

>If I got that right your saying I might fidget with the "conditions" but it won't matter if the stuff dosen't have the umph to begin with?
yes, if you change the agar formulation to improve the growth pattern of a PURE isolation, it won't make any difference in the final outcome, assuming all other conditions remain the same.


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hmmm........


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OfflinestonErollEr1
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Re: Problems getting Rhizo growth from MEA [Re: Champion des Champignons]
    #1092947 - 11/28/02 02:56 AM (14 years, 9 days ago)

But he was talking about multispore right?
In my experience MEA does not produce rhizo?s untill the 2:nd or 3:rd transfer..but if i put it on PDYA[yeast] it actually produces rhizo?s right away on multispore innoculation..
So my conclution is that yeast do stimulate rhizo growth and PDYA is my favorite agar recipe..since i usually can do an pretty clean isolation in 2-3 transfers.[without sectoring.]

that?s my 2cents..

peace...


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Invisiblezeta
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Re: Problems getting Rhizo growth from MEA [Re: stonErollEr1]
    #1092955 - 11/28/02 02:58 AM (14 years, 9 days ago)

How much yeast are you people using?


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OfflineAnnoA
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Re: Problems getting Rhizo growth from MEA [Re: stonErollEr1]
    #1092957 - 11/28/02 02:59 AM (14 years, 9 days ago)

>So my conclution is that yeast do stimulate rhizo growth and PDYA is my favorite agar recipe

Mine too.


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Offlinecujo7999
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Re: Problems getting Rhizo growth from MEA [Re: upupup]
    #1092993 - 11/28/02 03:14 AM (14 years, 9 days ago)

so what are all of the types of growth? i would just look for the biggest thickest quickest strands, right? but i understand that there are some names for the different types of growth, could i get a descriptions and names?

and if i were to do agar and powdered ...whatever theyed be growing on...Like dried and powdered grain, dung, or rice. then cook it with just agar. does this produce resonable growth and is it worth the effort to get substrate specific genetics isolated?


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*This is all Fiction by the Way*


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OfflinestonErollEr1
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Re: Problems getting Rhizo growth from MEA [Re: zeta]
    #1093005 - 11/28/02 03:22 AM (14 years, 9 days ago)

1g potato-powder [instant mashed potato-powder.Do you have that in the US?]
1g dextrose
2g Agar agar
0.2-0.4g dried yest

per 100ml H2o

Works great for me...
But everybody got their own favourite recipe..

pecae...


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InvisibleRoadkill
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Re: Problems getting Rhizo growth from MEA [Re: Anno]
    #1093195 - 11/28/02 04:54 AM (14 years, 9 days ago)

I like the PDYA the best too

5 grams instant potato's "Betty Crocker Potato Buds"
9 grams agar
7 grams of pure honey
1 gram of nutritional yeast mini flake
500 ml of purified water



--------------------
Laterz, Road

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OfflineSkikid16
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Re: Problems getting Rhizo growth from MEA [Re: Roadkill]
    #1093245 - 11/28/02 05:45 AM (14 years, 8 days ago)

In reply to:

7 grams of pure honey




This may sound stupid, but how does one go about measuring 7g of pure honey?


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Re-Defeat Bush in '04


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OfflineAnnoA
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Re: Problems getting Rhizo growth from MEA [Re: Skikid16]
    #1093260 - 11/28/02 06:27 AM (14 years, 8 days ago)

Weighing?


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OfflineSkikid16
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Re: Problems getting Rhizo growth from MEA [Re: Anno]
    #1093262 - 11/28/02 06:31 AM (14 years, 8 days ago)

So I just weigh it out? Damn I swear I'm retarded sometimes.


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Offlineupupup
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Re: Problems getting Rhizo growth from MEA [Re: Skikid16]
    #1093500 - 11/28/02 12:34 PM (14 years, 8 days ago)

Wow, great posts kids...

This helps immeasurably....lovin ya'll....


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OfflineOn_the_Down-Low
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Re: Problems getting Rhizo growth from MEA [Re: Anno]
    #1095394 - 11/29/02 03:45 AM (14 years, 8 days ago)

The armadillo was using the PDYA. But hasn't seen anything rhizomy in the first 7 days or so. There is some growth, but it can be hard to see it thru the 1/2 pint jars. He had noticed that some strains would pop out rhizomes that looked like a freaking forest of sea anemones on rye, but only produced cottony fluff on birdseed.


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InvisibleAlkaloids
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Re: Problems getting Rhizo growth from MEA [Re: Skikid16]
    #1095412 - 11/29/02 04:01 AM (14 years, 8 days ago)

don't worry.. the obvious can escape the best of us sometimes. *chuckle*


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OfflineOn_the_Down-Low
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Re: Problems getting Rhizo growth from MEA [Re: Skikid16]
    #1095435 - 11/29/02 04:20 AM (14 years, 8 days ago)

With water, 1 g = 1 ml.


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Paul Stamets, Rye Grain, Wild Bird Seed

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