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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 16 years, 9 months
flame me
    #1086541 - 11/26/02 02:02 AM (21 years, 4 months ago)

i know this is stupid...but how easy is it to get contams when making spore prints?

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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 19 years, 14 days
Re: flame me [Re: MsPacMan]
    #1086581 - 11/26/02 02:17 AM (21 years, 4 months ago)

Holy shit, are you a fucking retard, READ THE DAMN FAQs, shithead, ok ok, sorry, whew that felt good, hehehe, he you asked for it.

To answer your question, it all depends upon how clean you where in preparing the print itself. Did you use a clean printing surface? Did you cover the print while printing, if so did you clean the container used as a cover? Did you cut the stipe with clean scissors, did you handle the cap with clean hands? There are many, many variables to account for.


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Re-Defeat Bush in '04

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 6 months
Re: flame me [Re: MsPacMan]
    #1086584 - 11/26/02 02:18 AM (21 years, 4 months ago)

quite easy.

take some alu foil and cut it into square pieces. put them in a pan and ooopla! into the oven. Bake them for some time. Leave them there. When cooled, put the caps on each alu foil and put a glass on top of each. Leave them inside the oven [which is cool].


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The living ghost of Kykeon

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Invisiblezeta
Stranger

Registered: 05/24/02
Posts: 3,972
Re: flame me [Re: MsPacMan]
    #1086615 - 11/26/02 02:38 AM (21 years, 4 months ago)

In my experience it's quite hard to make a non-sterile print.

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 6 months
Re: flame me [Re: zeta]
    #1086650 - 11/26/02 02:58 AM (21 years, 4 months ago)

well, on my first crop, i was of NO need to make any prints, but i said, hey lets do it. I did it stoned, without thinkin. The result was a contaminated spore print.

Imho, STERILITY is the issue EVERYWHERE in mushroom cultivation.


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The living ghost of Kykeon

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OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 9,995
Loc: South Africa
Last seen: 19 minutes, 16 seconds
Trusted Cultivator
Re: flame me [Re: kykeon]
    #1086806 - 11/26/02 04:10 AM (21 years, 4 months ago)

I cut square tinfoil pieces and give them a good wiping down with rubbing alcohol.
That`s it.
seperatelyon a saucer with a glass over.

Edited by DERRAYLD (11/26/02 04:11 AM)

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InvisibleShaw

Registered: 06/27/01
Posts: 2,263
Re: flame me [Re: MsPacMan]
    #1086812 - 11/26/02 04:18 AM (21 years, 4 months ago)

i mainly fucked it up when doing 30cc syringes. I really don't remember before that.


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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 6 months
Re: flame me [Re: DERRAYLD]
    #1087095 - 11/26/02 08:23 AM (21 years, 4 months ago)

Quote:

I cut square tinfoil pieces and give them a good wiping down with rubbing alcohol.
That`s it.





:grin: well, i say, if u have an idea, share it with Derrayld. He has somethin better to say.

Cool! i prefer your method, no ovens and shit involved :smile: 


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The living ghost of Kykeon

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OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 9,995
Loc: South Africa
Last seen: 19 minutes, 16 seconds
Trusted Cultivator
Re: flame me [Re: kykeon]
    #1087111 - 11/26/02 08:27 AM (21 years, 4 months ago)

sarcasm will get you nothing but a warm smack upside the head my boy :wink:
lol

Mykeon my friend you`re a crasy one.

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Offlinerhizo
herb eater
Registered: 10/31/02
Posts: 599
Loc: Superposition of possible...
Last seen: 5 years, 7 months
Re: flame me [Re: kykeon]
    #1087115 - 11/26/02 08:29 AM (21 years, 4 months ago)

here are a couple of good basic printing teks. the first link is scotsman's and the second link the pf printing tek(both have pictures).

http://www.nansnook.com/forum/index.php?act=ST&f=17&t=565&s=05ccec9fa4e32c2d3215d97ab3dec525

http://www.fanaticus.com/syringe.htm


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An optimist is never pleasantly surprised.

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 6 months
Re: flame me [Re: DERRAYLD]
    #1087122 - 11/26/02 08:31 AM (21 years, 4 months ago)

Smack me HARD babe! :laugh: i like it like that... :laugh:

:tongue: 


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The living ghost of Kykeon

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: flame me [Re: MsPacMan]
    #1087123 - 11/26/02 08:31 AM (21 years, 4 months ago)

Very easy.

I make my prints in a glove box onto laminted index cards that have been wiped with alcohol. It's a bit of overkill but my prints are clean.  :smile: 


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 9,995
Loc: South Africa
Last seen: 19 minutes, 16 seconds
Trusted Cultivator
Re: flame me [Re: dog]
    #1087126 - 11/26/02 08:32 AM (21 years, 4 months ago)

Whipish!!!!

WHo`s your daddy?

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 6 months
Re: flame me [Re: DERRAYLD]
    #1087130 - 11/26/02 08:34 AM (21 years, 4 months ago)

uhm.... you?!?

lol

com'on daddy, do me like you did last year, hope u still can make me feel like flyin in heavens

:P:P:P:P:P


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The living ghost of Kykeon

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OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 9,995
Loc: South Africa
Last seen: 19 minutes, 16 seconds
Trusted Cultivator
Re: flame me [Re: kykeon]
    #1087134 - 11/26/02 08:36 AM (21 years, 4 months ago)

:tongue:  siss!!!

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OfflineBaby_Hitler
Errorist
 User Gallery

Folding@home Statistics
Registered: 03/06/02
Posts: 27,652
Loc: To the limit!
Last seen: 25 minutes, 20 seconds
Re: flame me [Re: MsPacMan]
    #1087592 - 11/26/02 12:16 PM (21 years, 4 months ago)

I think one of the biggest mistakes is that people get wet prints, then fold them up before letting them dry.

Wet prints are bad. Mmkay?

Umm.. Dumbass.  :wink:


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This space for rent

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