|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 Mushroom
Stranger
Registered: 11/25/02
Posts: 4
Last seen: 21 years, 4 months
|
 Inoculating without a syringe 
#1085408 - 11/25/02 07:34 PM (21 years, 4 months ago) |
|
|
Is there any way of inoculating without a syringe?
|
fredthetree
Stranger
Registered: 09/29/02
Posts: 473
Loc: Canada
Last seen: 18 years, 7 months
|
Re: Inoculating without a syringe [Re: Mushroom]
#1085441 - 11/25/02 07:50 PM (21 years, 4 months ago) |
|
|
yes.
|
rico357
journeyman
Registered: 11/24/02
Posts: 98
Last seen: 21 years, 1 month
|
Re: Inoculating without a syringe [Re: fredthetree]
#1085460 - 11/25/02 07:57 PM (21 years, 4 months ago) |
|
|
lol. great answer. roflmao! 
|
Mushroom
Stranger
Registered: 11/25/02
Posts: 4
Last seen: 21 years, 4 months
|
Re: Inoculating without a syringe [Re: fredthetree]
#1085483 - 11/25/02 08:04 PM (21 years, 4 months ago) |
|
|
ok...... well how?
|
user_exists
Stranger
Registered: 10/05/02
Posts: 501
Last seen: 20 years, 9 months
|
Re: Inoculating without a syringe [Re: Mushroom]
#1085489 - 11/25/02 08:06 PM (21 years, 4 months ago) |
|
|
You could innoculate using already grown mycelium but that would require an innoculated cake so just buy the syringe you cheap bastard (just fuckin joking man).
Edited by user_exists (11/25/02 08:07 PM)
|
New Name
New Name, OldUser
Registered: 11/11/02
Posts: 230
Loc: ATL
Last seen: 21 years, 3 months
|
Re: Inoculating without a syringe [Re: user_exists]
#1085503 - 11/25/02 08:12 PM (21 years, 4 months ago) |
|
|
I have heard of an eye dropper being used, but I haven't tried it myself.
--------------------
--------------------------------------------------------------------
"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.
|
 Bi0TeK
elephant man
Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
|
Re: Inoculating without a syringe [Re: Mushroom]
#1085525 - 11/25/02 08:23 PM (21 years, 4 months ago) |
|
|
I remember reading some tek where you just scrape some spore print onto the substrate.. It's in the "Grow/Find" section somewhere. Sorry I couldn't be more specific. Sounds a bit hit & miss to me though..
--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.
|
fredthetree
Stranger
Registered: 09/29/02
Posts: 473
Loc: Canada
Last seen: 18 years, 7 months
|
Re: Inoculating without a syringe [Re: Bi0TeK]
#1085544 - 11/25/02 08:27 PM (21 years, 4 months ago) |
|
|
yep, you can just scrape a bit of sporeprint off into the jar. actually works pretty well (i've used that method myself  ), it just takes a few more days to colonize.
but you should just go and buy some empty syringes! they're about $1 a peice
|
fredthetree
Stranger
Registered: 09/29/02
Posts: 473
Loc: Canada
Last seen: 18 years, 7 months
|
Re: Inoculating without a syringe [Re: Mushroom]
#1085551 - 11/25/02 08:29 PM (21 years, 4 months ago) |
|
|
sorry about my first reply
its just that your question is so vague, i felt i had to give it a vague answer
|
TeRzMaStA
Psilocybin Pimp
Registered: 10/08/01
Posts: 1,157
Last seen: 7 years, 1 month
|
Re: Inoculating without a syringe [Re: fredthetree]
#1085558 - 11/25/02 08:31 PM (21 years, 4 months ago) |
|
|
you can't just go and buy them though. Not unless you have a prescription. You could order them, but then you have to wait for them to get to your house. Last time I needed some I had to resort to convincing the guy at a mom and popo pet store to sell me some syringes. They were only 3cc too so I had to make a ton of em. Next time I went back for more there was a different guy and he wouldn't sell me any without a prescription.
--------------------
Think for yourself; Question Authority
Edited by TeRzMaStA (11/25/02 08:35 PM)
|
cookdandbombd
SERENITY NOW!
Registered: 08/31/02
Posts: 148
Loc: Northern Ireland
Last seen: 21 years, 2 months
|
Re: Inoculating without a syringe [Re: New Name]
#1085567 - 11/25/02 08:33 PM (21 years, 4 months ago) |
|
|
Yeah, before some kind people sent me syringe pins, I innoculated jars with an eye dropper, and a drinking straw attached to a syringe to act as the needle.
I sterilised them as best I could by pulling boiling water in and squirting out a number of times (I used water that had been boiled, covered, and was now cooling for the drinking straw method, so as not to warp the straw.)
And I had no probs with contams. That's using BRF and boiling the jars as I don't have a PC. HTH.
--------------------
--
Freak out baby, the bee is coming.
|
fredthetree
Stranger
Registered: 09/29/02
Posts: 473
Loc: Canada
Last seen: 18 years, 7 months
|
Re: Inoculating without a syringe [Re: TeRzMaStA]
#1085619 - 11/25/02 08:47 PM (21 years, 4 months ago) |
|
|
yeah i know, wtf is with that anyways? i ordered mine over the net  but a lot of people say you can go to an animal feed store and buy them (try and get 10cc syringes with 16/18/20 guage needles)
|
SixCee
keep rolling
Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
|
Re: Inoculating without a syringe [Re: fredthetree]
#1085690 - 11/25/02 09:03 PM (21 years, 4 months ago) |
|
|
I bet those people think you will shoot up with the needles or something.
When innoculating with a print use your oven. Turn it on and pull out one of the drawer things and work on that. The heat from the oven will destroy airborne contaminants.
--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.
|
zeta
Stranger
Registered: 05/24/02
Posts: 3,972
|
Re: Inoculating without a syringe [Re: Mushroom]
#1085856 - 11/25/02 09:50 PM (21 years, 4 months ago) |
|
|
I honestly woulnd't bother, the chance of success is so slim
|
Mushroom
Stranger
Registered: 11/25/02
Posts: 4
Last seen: 21 years, 4 months
|
Re: Inoculating without a syringe [Re: cookdandbombd]
#1085859 - 11/25/02 09:50 PM (21 years, 4 months ago) |
|
|
ok, well you see perhaps i can steel my schools eye dropper, its very thin, so it can go through the substrate properly. Now the other method of scraping the spores on, would i just scrap the spore print, and then rub it on the inside of the jar without the substrate in it, or with it in?
|
Anonymous
|
Re: Inoculating without a syringe [Re: Mushroom]
#1085976 - 11/25/02 10:21 PM (21 years, 4 months ago) |
|
|
Try to find a feed store. Most carry syringes, and big fuckers too. You dont need a prescription to give your horse a shot;)
|
PsiloSteve
Bioinformant
Registered: 04/07/00
Posts: 381
Last seen: 10 years, 10 months
|
Re: Inoculating without a syringe [Re: ]
#1086049 - 11/25/02 10:38 PM (21 years, 4 months ago) |
|
|
I used to inoculate by flecking spores right onto the substrate in a glovebox and really didn't have any problems with contam with most of my prints. Somehow a few of them were badly contaminated so I got me some agar. Instead of just making syringes out of the spores, or flecking directly onto the substrate, I would fleck the spores on some agar agar under sterile conditions. After it grew out a bit, I would then take a small piece of the mycelium and transfer it to another petri dish of agar with a sterile scalpel until an isolated culture was obtained. Then I would take that final mycelium and drop in a dish of sterile water and suck up with a syringe.
Voilah, mycelium syringes! Glovebox is a necessity for working with agar however but thats no big deal to make.
--------------------
Don't worry be happy!
|
PsiloSteve
Bioinformant
Registered: 04/07/00
Posts: 381
Last seen: 10 years, 10 months
|
Re: Inoculating without a syringe [Re: PsiloSteve]
#1086070 - 11/25/02 10:44 PM (21 years, 4 months ago) |
|
|
Oh shit that's right you said without a syringe, well you could just scrape up the mycelium culture in the glovebox with a scalpel and drop in the jar, or you could just fleck spores, but I would definately invest the time in making a glovebox. Definately worth the time IMO. 
--------------------
Don't worry be happy!
|
bugabuga420
bUgAbUgaBOO
Registered: 07/18/02
Posts: 488
Last seen: 6 years, 5 months
|
Re: Inoculating without a syringe [Re: PsiloSteve]
#1086078 - 11/25/02 10:48 PM (21 years, 4 months ago) |
|
|
I know for a fact you can get them at local feed stores. I get mine at a chain called tractor supply which just carries like garden equipment and all types of pet foods and a large animal vet section. They have premade syringes with mediicine and have all types of empty syringes and needles. I never got the courage to go up and buy them though just becuase i dont look like a farmer type at all. They need to invest in security 
|
DERRAYLD
Constructus
Registered: 05/13/02
Posts: 9,980
Loc: South Africa
Last seen: 8 hours, 34 minutes
|
Re: Inoculating without a syringe [Re: bugabuga420]
#1086086 - 11/25/02 10:51 PM (21 years, 4 months ago) |
|
|
I get mine at the chemist.
Obviously here we have less junkies I`m guessing.
So I just tell them I use them to innoculate jars of substrate to grow mushrooms.
They give me a confused look and just point me to the syringes and needles.
Now when I go in I just go and fetch my own from the despensary, no one helps me any more. Thye pretty much know what I want by now.
|
|
Threaded
Previous
Index
Next
Edit 
Reply 
Fraggin
kiss
Zwieback0
GnuBobo
1 2 3 4 all )
EaTmEKiDs
Salvinorin
discman1