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Amazon Shop: ½ Pint Jars, Brown Rice Flour

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OfflineNoOne
Ca-razy
Registered: 11/09/02
Posts: 7
Last seen: 14 years, 6 months
No Growth?
    #1083589 - 11/25/02 04:23 AM (14 years, 6 months ago)

well i've done my first attempt here now....i've followed everything to a tee and it's been about 3 n a half weeks and no sign of anything.....i'm usin the brf teck any advice here?


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Invisiblecarbonhoots
old hand

Registered: 09/11/01
Posts: 1,351
Loc: BC Canada
Re: No Growth? [Re: NoOne]
    #1083610 - 11/25/02 04:32 AM (14 years, 6 months ago)

If your keeping it warm...it's contaminated


--------------------
  -I'd rather have a frontal lobotomy than a bottle in front of me

CANADIAN CENTER FOR POLICY ALTERNATIVES


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OfflineNoOne
Ca-razy
Registered: 11/09/02
Posts: 7
Last seen: 14 years, 6 months
Re: No Growth? [Re: carbonhoots]
    #1083619 - 11/25/02 04:39 AM (14 years, 6 months ago)

.....i am keeping it warm at around 75 80 degrees but there's no growth what so ever, how can it be contaminated? there's no signs of anything at all


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OfflineDobie
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Registered: 08/15/02
Posts: 52,140
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Re: No Growth? [Re: NoOne]
    #1083623 - 11/25/02 04:43 AM (14 years, 6 months ago)

did ya sterilze after you injected the spores or before


--------------------
This place is gayer than when the balls touch


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OfflineNoOne
Ca-razy
Registered: 11/09/02
Posts: 7
Last seen: 14 years, 6 months
Re: No Growth? [Re: Dobie]
    #1083628 - 11/25/02 04:46 AM (14 years, 6 months ago)

before of course...i followed directions exactly, why would i want to streilize and kill my spores? geez i'm not incompident


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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/18/99
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Re: No Growth? [Re: NoOne]
    #1083639 - 11/25/02 05:01 AM (14 years, 6 months ago)

>i'm not incompident
* incompetent

Do the jars smell?


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OfflineNoOne
Ca-razy
Registered: 11/09/02
Posts: 7
Last seen: 14 years, 6 months
Re: No Growth? [Re: Anno]
    #1083645 - 11/25/02 05:08 AM (14 years, 6 months ago)

sorry i'm not that smart at 2am....no smell no nothin!


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OfflineJackal
Well Versed In Etiquette
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Registered: 10/16/02
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Re: No Growth? [Re: Anno]
    #1083656 - 11/25/02 05:15 AM (14 years, 6 months ago)

In reply to:

>i'm not incompident
* incompetent




Ahahahahahahahahahaaaa
ROFLMFAO
Ahahahahahahahahahaaaa
Sorry just found that hysterical - It is Monday morning!


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OfflineAnnoA
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Re: No Growth? [Re: NoOne]
    #1083701 - 11/25/02 06:18 AM (14 years, 6 months ago)

Were the jars completely cool when you inoculated them?
Perhaps the spores were old?


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OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
Last seen: 8 years, 6 months
Re: No Growth? [Re: Anno]
    #1083827 - 11/25/02 08:42 AM (14 years, 6 months ago)

I`m willing to bet the spores were old as Anno said.


--------------------
Derrayld and out of control


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OfflineNoOne
Ca-razy
Registered: 11/09/02
Posts: 7
Last seen: 14 years, 6 months
Re: No Growth? [Re: Anno]
    #1084201 - 11/25/02 01:49 PM (14 years, 6 months ago)

it was completely cooled down, yeah maybe the spores were old....anyway back to the drawing board huh? here's a question though...would that jar still be ok to put some new spore into? or should i start over, nothing has grown or anything so it should still be sterile though right? would it be safe to innoculate again?


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Offlineshroominbloom
i have arrived

Registered: 11/18/02
Posts: 457
Last seen: 11 years, 2 months
Re: No Growth? [Re: NoOne]
    #1084226 - 11/25/02 01:58 PM (14 years, 6 months ago)

Yeah, as soon as you boil or PC them again. Don't take any chances to save time, it's not going to be worth it in the long run cause you might get the same thing all over again. 12hrs<3weeks


--------------------
ha


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Amazon Shop: ½ Pint Jars, Brown Rice Flour

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