Home | Community | Message Board


Shroom Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Pressure Cooker

Jump to first unread post. Pages: 1
OfflineYellowSubmarine
Soviet Pig

Registered: 10/09/02
Posts: 946
Loc: U.S.S.R.
Last seen: 12 years, 27 days
Sterile Syringes
    #1083552 - 11/25/02 03:56 AM (15 years, 4 months ago)

Hey all-

I just made three syringes. I put the cap into a 1/2 pint jar. The jar sat in the oven at 350 degrees for 30 minutes and cooled before I put the cap in. After 2 days, the cap was quickly removed. I then took three syringes and drew in boiling water, then wrapped the syringes in foil and threw them into the foil to cook up for an hour. Let them cool in the fridge for 30 minutes then squirted the water into the jar and mixed it all up by swirling the jar around. I drew up the spores and voila. Question is, how sterile do you have to be when making syringes. Are the problems with contamination as equal as the contamination possibilites once innocluating?? Can little bacteria get up inside the syringe, hang out then attack your cakes??? Has this happened to anyone?

just a little paranoid


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepleezr
fuct

Registered: 12/22/01
Posts: 1,708
Loc: NW
Last seen: 13 years, 2 months
Re: Sterile Syringes [Re: YellowSubmarine]
    #1083665 - 11/25/02 05:27 AM (15 years, 4 months ago)

Were your syringes used? If so, you may have wanted to draw boiling water a few times then let it sit until it cools. You did let them cool right?

If they were sealed and sterile in the first place, your fine.


--------------------
pleezr

"Tell me about the fucking golf shoes!!"


Edited by pleezr (11/25/02 05:28 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineYellowSubmarine
Soviet Pig

Registered: 10/09/02
Posts: 946
Loc: U.S.S.R.
Last seen: 12 years, 27 days
Re: Sterile Syringes [Re: pleezr]
    #1083678 - 11/25/02 05:44 AM (15 years, 4 months ago)

they weren't used and yeah I let them cool in the fridge for like 30 minutes


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepleezr
fuct

Registered: 12/22/01
Posts: 1,708
Loc: NW
Last seen: 13 years, 2 months
Re: Sterile Syringes [Re: YellowSubmarine]
    #1086800 - 11/26/02 06:05 AM (15 years, 4 months ago)

If you are using new sterile syringes. I would recomend sterilizing your water in a pressure cooker and letting it cool to room temperature normally. Whether you boil it or sterilize it, I dont think putting clean water in to a dirty refigerator is such a good idea. The syringes are already clean and sterile, dont go through the trouble of sterilizing the syringes themselves unless they have been used and are being resused.

Just a tip


--------------------
pleezr

"Tell me about the fucking golf shoes!!"


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Success rate at first time making a sterile syring tekramrepus 2,939 9 09/18/02 10:23 PM
by PSILLYSIMON
* Loading up substrate and sterilizing later? liftedoff420 779 16 02/20/03 04:06 AM
by blackout
* Question about sterility??? VIgnisFatuusI 985 14 07/10/03 10:58 PM
by VIgnisFatuusI
* Sterile jars flawed 805 4 11/04/02 10:47 PM
by repobob
* Post deleted by Moe Howard Anonymous 390 2 11/06/02 06:22 PM
by zeta
* Re: sterilization of spore print AnubisRonin 1,409 10 01/28/00 01:22 PM
by Anonymous
* contamination problem abraham 939 3 11/07/02 12:51 PM
by abraham
* Need advice on sterility & trading spores. The_Gnome_King 1,607 14 06/05/01 12:16 AM
by Azure

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
577 topic views. 26 members, 68 guests and 9 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Avalon Magic Plants
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.008 seconds on 19 queries.