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Offlineporsches
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Registered: 11/15/02
Posts: 2
Last seen: 21 years, 3 months
Bacterie on sclerotia that is sold?
    #1079856 - 11/23/02 05:08 PM (21 years, 4 months ago)

hi there,

I like to know that sclerotia vendors put a good bacterie on the sclerotioa to prevent cloning.
when tryng to grow some byed ones in Amsterdam on agar i noticed a jelly stuff on all agar plates.

All help much apriciated
thanks
P

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InvisibleShaw

Registered: 06/27/01
Posts: 2,257
Re: Bacterie on sclerotia that is sold? [Re: porsches]
    #1080749 - 11/24/02 12:31 AM (21 years, 4 months ago)

You say your attempting to clone sclorotia bought in amsterdam? I would assume they are using a chemical to prevent growth, like they do on garlic, and other things. I have not heard of any bacteria used to hault growth. What species are you working with? I would just buy the spores and grow them out on rye. Was the sclorotia you bought dryed or fresh?


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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 4 months, 22 days
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Re: Bacterie on sclerotia that is sold? [Re: Shaw]
    #1081236 - 11/24/02 05:58 AM (21 years, 4 months ago)

i heard of them contaminating them with "friendly" bacteria to stop cloning. they want you to buy spores and the stones are cheaper than spores in some cases. i cant remember where i read this but i am sure i did...

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OfflineNeonBlack
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Registered: 11/23/00
Posts: 331
Last seen: 14 years, 7 months
Re: Bacterie on sclerotia that is sold? [Re: porsches]
    #1081364 - 11/24/02 09:24 AM (21 years, 4 months ago)

You know, I'm sure that the sclerotia were not handled in sterile conditions and packaged in a sterile container.. It's more likely that they just picked up some bacteria from the air or someone's hand.

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OfflineWorkmanV
1999 Spore War Veteran
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Registered: 03/01/01
Posts: 3,601
Loc: Oregon, USA
Last seen: 1 hour, 40 minutes
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Re: Bacterie on sclerotia that is sold? [Re: blackout]
    #1081370 - 11/24/02 09:30 AM (21 years, 4 months ago)

I find that highly unlikely. Normal handling would place enough bacteria on the surface to make cloning a bit more difficult but I have heard that it can be done. I don't think sclerotia vendors would bother with placing growth inhibiting materials on sclerotia since serious cultivators can purchase tampanesis and mexicana spores/cultures. The extra expense and handling wouldn't be cost effective. Most sclerotia buyers are not interested in cultivation.


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Offlineporsches
Stranger
Registered: 11/15/02
Posts: 2
Last seen: 21 years, 3 months
Re: Bacterie on sclerotia that is sold? [Re: Shaw]
    #1081853 - 11/24/02 01:40 PM (21 years, 4 months ago)

Hi,

The ones where Tampanensis fresh and cleaned with 30% peroxide.
Any idea how i could see bacteria contams in the liquid.
I supose with a microscoop but yeah how would they look like?

Thanks again
P

Edited by porsches (11/24/02 01:42 PM)

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