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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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OfflineShpongloid
WBS-o-phile

Registered: 11/16/02
Posts: 196
Last seen: 8 years, 7 months
Recommended Inoculation Amounts?
    #1081359 - 11/24/02 11:22 AM (14 years, 3 months ago)

Hello, just wondering how many cc's you guys and gals recommend for 1/2 pint substrate jar and for a 1 pint substrate jar.


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"...NIDA-supplied marijuana cigarettes weigh approximately .9 grams. A smoker would theoretically have to consume nearly 1,500 pounds of marijuana within about fifteen minutes to induce a lethal response."


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Offlinemelic
undead
Registered: 02/21/02
Posts: 85
Loc: fin
Last seen: 11 years, 11 months
Re: Recommended Inoculation Amounts? [Re: Shpongloid]
    #1081373 - 11/24/02 11:32 AM (14 years, 3 months ago)

About 1cc per jar should be fine


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Offlinerhizo
herb eater

Registered: 10/31/02
Posts: 598
Loc: Asia
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Re: Recommended Inoculation Amounts? [Re: Shpongloid]
    #1081500 - 11/24/02 01:02 PM (14 years, 3 months ago)

I prepare my substrate a little drier and then inoculate with about 10 cc's per jar, 2.5 cc's per inoculation point, using a 50 ml syringe(only good for 5 jars but they colonize quickly). I usually get 100% surface colonization in around 8 or 9 days, much slower if the substrate is too wet.

[edit]oh yeah, I use this for 1/2 pints, i don't use pints[/edit]


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An optimist is never pleasantly surprised.


Edited by rhizo (11/24/02 01:07 PM)


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Offlinesdrussell
newbie
Registered: 11/02/02
Posts: 33
Last seen: 14 years, 2 months
Re: Recommended Inoculation Amounts? [Re: rhizo]
    #1081515 - 11/24/02 01:09 PM (14 years, 3 months ago)

I have been getting 100% colonization at about 6-8 days with less than 1 cc per jar. I do 12 jars with a 10cc syringe. Anymore than 1 cc would be a waste. I( incubate my jars between 87-89 degrees. That seems to work better for me than 85.


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Offlinerhizo
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Registered: 10/31/02
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Re: Recommended Inoculation Amounts? [Re: sdrussell]
    #1081534 - 11/24/02 01:18 PM (14 years, 3 months ago)

it's not a waste if you've got loads of mycelial solution, I like to use it fresh, i just prepare and shoot. i find it hard to believe you're getting 100% at 6 days using less than 1 cc per jar...what are you shooting, 1/8 pints?


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An optimist is never pleasantly surprised.


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
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Re: Recommended Inoculation Amounts? [Re: sdrussell]
    #1081614 - 11/24/02 01:41 PM (14 years, 3 months ago)

6 - 8 days? What exactly are you doing to get your cakes colonized so quickly?


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Offlinerhizo
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Registered: 10/31/02
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Re: Recommended Inoculation Amounts? [Re: sdrussell]
    #1081636 - 11/24/02 01:47 PM (14 years, 3 months ago)

"Anymore than 1 cc would be a waste"

it's only a waste if you don't know how to make more


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OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
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Re: Recommended Inoculation Amounts? [Re: rhizo]
    #1081687 - 11/24/02 02:22 PM (14 years, 3 months ago)

in 6-8 days 100% colonised?!?!?!!


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Offlinesdrussell
newbie
Registered: 11/02/02
Posts: 33
Last seen: 14 years, 2 months
Re: Recommended Inoculation Amounts? [Re: Hamurabi]
    #1082053 - 11/24/02 05:31 PM (14 years, 3 months ago)

Yeah. They have been becomming fully colonized in about 6-8 days. The first batch I did, I used 10cc's to innoculate 12 jars. 8 of them were fully colonized in 6 days. Only 1 of them was outside the 8 day window. I am using widemouth halfpints and incubating them at about 87-89 degrees. It is not a fluke either, because I innoculated more on the 18th, and those are about 85-90 percent.
BTW, using PFC's.


Edited by sdrussell (11/24/02 05:43 PM)


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Offlinesdrussell
newbie
Registered: 11/02/02
Posts: 33
Last seen: 14 years, 2 months
Re: Recommended Inoculation Amounts? [Re: rhizo]
    #1082061 - 11/24/02 05:36 PM (14 years, 3 months ago)

In reply to:

"Anymore than 1 cc would be a waste"

it's only a waste if you don't know how to make more




I do know how to make more. I just made 4 syringes last night. Why use more solution than you need to and just have to waste time making more syringes?


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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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