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Offlineporsches
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Registered: 11/15/02
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Last seen: 13 years, 11 months
Bacterie on sclerotia that is sold?
    #1079856 - 11/23/02 07:08 PM (14 years, 21 days ago)

hi there,

I like to know that sclerotia vendors put a good bacterie on the sclerotioa to prevent cloning.
when tryng to grow some byed ones in Amsterdam on agar i noticed a jelly stuff on all agar plates.

All help much apriciated
thanks
P


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OfflineShaw
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Registered: 06/28/01
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Re: Bacterie on sclerotia that is sold? [Re: porsches]
    #1080749 - 11/24/02 02:31 AM (14 years, 20 days ago)

You say your attempting to clone sclorotia bought in amsterdam? I would assume they are using a chemical to prevent growth, like they do on garlic, and other things. I have not heard of any bacteria used to hault growth. What species are you working with? I would just buy the spores and grow them out on rye. Was the sclorotia you bought dryed or fresh?


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Offlineblackout
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Re: Bacterie on sclerotia that is sold? [Re: Shaw]
    #1081236 - 11/24/02 07:58 AM (14 years, 20 days ago)

i heard of them contaminating them with "friendly" bacteria to stop cloning. they want you to buy spores and the stones are cheaper than spores in some cases. i cant remember where i read this but i am sure i did...


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OfflineNeonBlack
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Re: Bacterie on sclerotia that is sold? [Re: porsches]
    #1081364 - 11/24/02 11:24 AM (14 years, 20 days ago)

You know, I'm sure that the sclerotia were not handled in sterile conditions and packaged in a sterile container.. It's more likely that they just picked up some bacteria from the air or someone's hand.


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OfflineWorkmanV
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Re: Bacterie on sclerotia that is sold? [Re: blackout]
    #1081370 - 11/24/02 11:30 AM (14 years, 20 days ago)

I find that highly unlikely. Normal handling would place enough bacteria on the surface to make cloning a bit more difficult but I have heard that it can be done. I don't think sclerotia vendors would bother with placing growth inhibiting materials on sclerotia since serious cultivators can purchase tampanesis and mexicana spores/cultures. The extra expense and handling wouldn't be cost effective. Most sclerotia buyers are not interested in cultivation.


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Offlineporsches
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Registered: 11/15/02
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Re: Bacterie on sclerotia that is sold? [Re: Shaw]
    #1081853 - 11/24/02 03:40 PM (14 years, 20 days ago)

Hi,

The ones where Tampanensis fresh and cleaned with 30% peroxide.
Any idea how i could see bacteria contams in the liquid.
I supose with a microscoop but yeah how would they look like?

Thanks again
P


Edited by porsches (11/24/02 03:42 PM)


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