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Offlinebowling-name
sleuth

Registered: 09/24/02
Posts: 282
Loc: Mirror in the Sky
Last seen: 7 years, 5 months
Thermal death: visual vs. behavioral determination
    #1073865 - 11/21/02 03:19 PM (18 years, 8 months ago)

October 15th: Four mycobags were innoculated with T.H.E.'s Koh Samui strain multispore solution. Lost one bag quickly to green mold, but the other three were colonizing at a reasonable pace (at room temperature ~70F in complete darkness) considering my heater failed.

About a week ago I took a look at them, and to my horror discovered that the aquarium heater had come back on and was overheating the incubation environment to approximately 105 degrees! I hadn't peaked for at least a week before this so I'm not sure how long they were subjected to these temperatures.

Since then: one bag definitely died (exuding a strange rust colored mold at the point of innoculation), and one bag probably died (no signs of healthy mycelium recolonization post-shaking). The mycelium in the third bag still looks white and somewhat rhizomorphic, albeit only 20% colonized or so.

Is (apparent) cessation of growth proof that mycelium is dead, even when it looks fairly healthy? These Thai strains are fairly robust when it comes to heat (a couple years ago, PF-tek-grown Cambodian cubes were fruited following a similar incubation overheating mishap), so I wonder if this bag still has a chance...any suggestions? Should I reinnoculate the one healthy bag (I have a couple CCs of spore solution remaining)? Sorry no pics. Thanks in advance.


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 16 years, 3 months
Re: Thermal death: visual vs. behavioral determination [Re: bowling-name]
    #1073890 - 11/21/02 03:26 PM (18 years, 8 months ago)

If the mycelium still appears to be healthy in your one bag, I say just leave it be in the correct incubation temps, re-inoculating may introduce contams.


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Re-Defeat Bush in '04


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