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OfflineAuditory Misperception
Digital Hippy
Registered: 11/15/99
Posts: 32
Last seen: 21 years, 6 months
Shaking jars of rye
    #1070808 - 11/20/02 04:04 PM (21 years, 7 months ago)

After Inoculating some rye jars I shook them to spread out the spores, was I supose to do this, or is spreading the spores out a bad idea?

It's been about 48 hours and I don't see any groth yet, I'm probably just being impatient and paranoid...

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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 17 years, 5 days
Re: Shaking jars of rye [Re: Auditory Misperception]
    #1070880 - 11/20/02 04:16 PM (21 years, 7 months ago)

yes you were supposed to shake it, wait longer.

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OfflineJovialLeprechaun
reasonableperson
Registered: 09/04/02
Posts: 276
Last seen: 17 years, 4 months
Re: Shaking jars of rye [Re: Auditory Misperception]
    #1070909 - 11/20/02 04:23 PM (21 years, 7 months ago)

Somewhere in the FAQ there is a section that recommends when to shake the jars, it's not going to hurt it at the start - I believe it's something like 20% you shake once and then dont shake again. It's just to move the colonized bits around so you're not waiting for a large patch of uncolonized grain to get done at the end. With many points of colo'd grain working towards it, it takes much less time.

I made that mistake the other day, the other jars which were shaken colonized in about 7 days, but the one jar that I forgot to shake is still going (12 days).
This isnt from spores however, its from a grain to grain transfer, so dont be discouraged if you dont see much in a week - when I originally started grains it seemed to take forever for the spores to germinate.. but once they do! zoom.


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I am a reasonable person.

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Offlinenksfo5
traverse traveler

Registered: 12/19/12
Posts: 689
Last seen: 4 months, 1 day
Re: Shaking jars of rye [Re: Auditory Misperception]
    #17898253 - 03/03/13 04:13 PM (11 years, 3 months ago)

waiting is the majority of this hobby

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InvisibleKavince

Registered: 02/22/13
Posts: 250
Re: Shaking jars of rye [Re: nksfo5]
    #17898283 - 03/03/13 04:19 PM (11 years, 3 months ago)

but when you get to do something it's amazingly fun


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Fuck the King's Guard. Fuck the city. Fuck the King.
-The Hound

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OfflineJabbawaya

Registered: 07/10/05
Posts: 1,479
Last seen: 10 years, 6 months
Re: Shaking jars of rye [Re: Kavince]
    #17898314 - 03/03/13 04:27 PM (11 years, 3 months ago)

I've had the most success injecting the spores into a single spot in the jar only, and then shaking at estimated 10-20% colonization. It will colonize very quickly after that, as myceliated rye is distributed throughout the jar. Shaking the jar right after injecting the spores can slow germination, but it will still germinate.

For liquid cultures, the jar is best shaken right after inoculation, because you're dealing with live mycelium instead of spores, which would have to germinate.

Don't worry about no colonization after 48 hours at 68 - 78 *F. It could take up to a week, or even two weeks, depending on the strain. Plus, a watched pot never boils :tongue:


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 6 days
Trusted Cultivator
Re: Shaking jars of rye [Re: Jabbawaya]
    #17898325 - 03/03/13 04:31 PM (11 years, 3 months ago)

Quote:

Jabbawaya said:
It could take up to a week, or even two weeks, depending on the strain spores. Plus, a watched pot never boils :tongue:



Spores can take up to 3 weeks to germinate in some cases.

It has nothing to do with the "strain". Strain in mushroom cultivation is not the same as strain in pot growing.

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OfflineJabbawaya

Registered: 07/10/05
Posts: 1,479
Last seen: 10 years, 6 months
Re: Shaking jars of rye [Re: PussyFart]
    #17898341 - 03/03/13 04:35 PM (11 years, 3 months ago)

I agree in principle, and while I agree that "spores are spores", I do notice that my Texas prints consistently take a week and a half longer to germinate than my Panama prints, all things being equal. I'm not really sure why, it could come down to a number of factors.


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