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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Resurrecting dried shrooms on cardboard :)
#10708073 - 07/20/09 06:58 AM (15 years, 5 months ago) |
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This is a continuation and small re-cap from my other post as it was getting a bit off topic in "Mushroom Hunting and Identification" My old post - MY FIRST SHROOMS!!! plz help identify.
Obtained 100 dried Subs (Psilocybe Subaeruginosa) off a mate... well nearly fully dried, the stems were completely dry but the tops still had a small amount of flex in them. Then picked out 4 of my biggest that felt the most flexy, in the hope that they contained some living tissue or be mature enough to have seeded a few spores.
 I then went about soaking some cardboard for 10-15mins in my pond (Maybe not the most sterile choice..... but cbf filling up a container to soak it in at the time.)
 Next i peeled apart the cardboard and placed my lucky 4 subs
 & finally Placed the cardboard somewhere cool, damp and away from direct sunlight. It may be a good idea to place a board of wood or something underneath to give other bacteria less of a chance at the cardboard, but i was over scrabbling around under that bush so i left it as is.
They were placed there last thursday (16/7/09) will b checking them this thursday coming Hopefully this is going to work and ill see some mycelium starting to grow by then.
My technique is based on the link "karode13" was kind enough to sending me. (cheers big help) Make cardboard spawn with all of your finds! Tutorial.
Edit: Repairing broken photo links.
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Edited by Ambient Forest (06/14/10 06:09 AM)
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CubieMcCuberton

Registered: 03/19/09
Posts: 781
Loc: ation
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10708088 - 07/20/09 07:07 AM (15 years, 5 months ago) |
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I'm sorry man, but this is not going to work. This is somewhat difficult to do even with healthy living mushroom tissue, and you are trying to do it with whole dried mushrooms, that probably have VERY little or more likely no living tissue.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: CubieMcCuberton]
#10708143 - 07/20/09 07:31 AM (15 years, 5 months ago) |
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doh!.... lol yeah u are probs right, a few other ppls said similar in my other post, but fingers crossed. I took a photo last sunday (3 nights after placing them) (impatient to see if there was any progress)
  (in person the myc looks a clean white not the sorta blue/green tinge one of the photos) oh and those lumps are the bases of a few subs that were cased in dirt and dried myc that i placed in when i took these two photos.
what do ya reckon..... sub mycelium or common mould....? hope its sub myc but have a bad feeling it is mould.....
Edit: Repairing broken photo links.
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Edited by Ambient Forest (06/14/10 06:13 AM)
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karode13
Tāne Mahuta




Registered: 05/19/05
Posts: 15,343
Loc: LV-426
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10708179 - 07/20/09 07:41 AM (15 years, 5 months ago) |
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It looks like mould in my opinion and you should be using fresh stem butts to begin with. 10/10 for trying 
This method does work for wood lovers if you use fresh material and is virtually idiot proof. Really try to find some fresh ones before the season is over.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: karode13]
#10708223 - 07/20/09 08:04 AM (15 years, 5 months ago) |
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Trying to get the crew together 4 a hunt this sunday, now that i have a little more idea of what I'm looking 4. Will put up photos of our catch if we succeed 
Going 2 b perfect conditions, has been pissing down with rain lately and the weekend is when it starts 2 clear. hopefully theirs not 2 many ppls with the same idea as me, and end up raiding it on saturday...
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prismism



Registered: 05/11/09
Posts: 5,570
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10708296 - 07/20/09 08:36 AM (15 years, 5 months ago) |
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listen man. if you are going to pick them wild anyway then just take a print out in the field and then cultivate from that print. why do it the hard way?
-------------------- ephemeral anomalous
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CubieMcCuberton

Registered: 03/19/09
Posts: 781
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10708324 - 07/20/09 08:44 AM (15 years, 5 months ago) |
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Ya that looks like mold to me. If you are gonna use fresh tissue make sure you are a little more clean i.e. dont soak the cardboard in your pond water lol, and you may have some luck.
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karode13
Tāne Mahuta




Registered: 05/19/05
Posts: 15,343
Loc: LV-426
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Re: Resurrecting dried shrooms on cardboard :) [Re: prismism]
#10708372 - 07/20/09 09:01 AM (15 years, 5 months ago) |
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Quote:
prismism said: listen man. if you are going to pick them wild anyway then just take a print out in the field and then cultivate from that print. why do it the hard way?
This is the easiest way for a beginner with no growing experience to start growing wood lovers if they have access to fresh material. No need for sterile techniques, just wet cardboard in clean water, place stem butts in between and in 2-3 days the fine mycelium threads on the end of the stem starts to grow. In about two weeks transfer colonized cardboard and mix with wood chips. It has been done numerous times and is too easy.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
Loc:
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Re: Resurrecting dried shrooms on cardboard :) [Re: CubieMcCuberton]
#10708403 - 07/20/09 09:10 AM (15 years, 5 months ago) |
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hahah yeah pond water was a bit dodge....
Maybe a good thing if it doesn't work as they were weak as fk. me and 3 mates devoured the 100 between us and only got a mild trip, felt like an ok pill & things were a bit trippy like occasional small visual distortion out of the corner of ur eye, but not much other than that. Or maybe my expectations are 2 high...? as that was my first shroom trip.
Maybe it was caus they weren't dried properly or 2 old, as i don't know when they were picked.
mmmmm picking myself definitely seems to be the best solution, just gotta find em.
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Stealthgrower
n00b Pwner



Registered: 04/19/09
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Re: Resurrecting dried shrooms on cardboard :) [Re: prismism]
#10712430 - 07/20/09 11:04 PM (15 years, 5 months ago) |
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Quote:
prismism said: listen man. if you are going to pick them wild anyway then just take a print out in the field and then cultivate from that print. why do it the hard way?
He said he got some old dried ones from someone else. And just so everyone knows, cardboard is not JUST for woodlovers. I put chunks from nice looking fat outdoor cubes on cardboard to transfer and clone.
-------------------- Infea said:
You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
Loc:
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Re: Resurrecting dried shrooms on cardboard :) [Re: Stealthgrower]
#10713382 - 07/21/09 05:24 AM (15 years, 5 months ago) |
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Schweet its locked in, me n the crew are going down this sunday
hopefully we will find some subs that are a lil more potent... or find anything... lol
Should i worry about my tolerance been kicked in the ass from last saturday..... i would say we had a level one trip, from what i've read.
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10713402 - 07/21/09 05:34 AM (15 years, 5 months ago) |
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Quote:
I'm sorry man, but this is not going to work. This is somewhat difficult to do even with healthy living mushroom tissue, and you are trying to do it with whole dried mushrooms, that probably have VERY little or more likely no living tissue.
it is totally possible, i reserected a dries 3 year old azure.
on agar mind, keep trying, you might get a nice peice of myc to grow ;0
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: FractalXplora]
#10713416 - 07/21/09 05:47 AM (15 years, 5 months ago) |
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ah true hmmmm, gettin my hopes up haha
is there any sure fire way to tell if it is mould or sub myc....? other than having small mushrooms starting to grow off of it.... :P
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TsalagiSpirit
Reality Shattered



Registered: 10/01/08
Posts: 587
Loc: AK-49
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10713420 - 07/21/09 05:51 AM (15 years, 5 months ago) |
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Demz is ams cobwebz.
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Caution: All information on this post or from this account are fictional and images are for entertainment purposes only. I do not condone the use, sale, manufacture or distributation of controlled substances in any country.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
Loc:
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Re: Resurrecting dried shrooms on cardboard :) [Re: TsalagiSpirit]
#10713430 - 07/21/09 05:57 AM (15 years, 5 months ago) |
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I'm hell noob, what's "ams cobwebz"?
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ArcanePerception
The only rule isthe golden rule



Registered: 04/30/05
Posts: 435
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Re: Resurrecting dried shrooms on cardboard :) [Re: FractalXplora]
#10713432 - 07/21/09 05:57 AM (15 years, 5 months ago) |
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cardboard is pretty selective against molds he might have some luck and it didnt seem like it took to much cost or effort I dont understand why people are being so negative about it.
-------------------- Oh, welcome to this world of fools
Of pink champagne and swimming pools
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TsalagiSpirit
Reality Shattered



Registered: 10/01/08
Posts: 587
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10713436 - 07/21/09 05:59 AM (15 years, 5 months ago) |
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Sorry, I'm using my swedish voice. It looks like cobweb for sure though. I dunno, keep posting.
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Caution: All information on this post or from this account are fictional and images are for entertainment purposes only. I do not condone the use, sale, manufacture or distributation of controlled substances in any country.
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TsalagiSpirit
Reality Shattered



Registered: 10/01/08
Posts: 587
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Re: Resurrecting dried shrooms on cardboard :) [Re: TsalagiSpirit]
#10713439 - 07/21/09 06:01 AM (15 years, 5 months ago) |
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One way to tell if its mushroom mycelium is to bruise it. Subs will stain blue really quickly, though you may contaminate it by touching it.
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Caution: All information on this post or from this account are fictional and images are for entertainment purposes only. I do not condone the use, sale, manufacture or distributation of controlled substances in any country.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
Loc:
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Re: Resurrecting dried shrooms on cardboard :) [Re: TsalagiSpirit]
#10713494 - 07/21/09 06:34 AM (15 years, 5 months ago) |
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ahk cheers, thats really helpful ill try to remember to give my hands a good wash b4-hand once there is a good lot of myc for me to poke :P
mmmm the rain has stopped, think i might grab the torch and trek it out through my garden c how they are looking will have photos up soon.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10713526 - 07/21/09 06:58 AM (15 years, 5 months ago) |
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well.... something having a go on em..... lol staying positive, fingers crossed, even if they eventually don't turn out I'm enjoying living in hope haha 
   
Edit: Repairing broken photo links.
Edited by Ambient Forest (06/14/10 06:15 AM)
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UpSwell
Stranger

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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10714168 - 07/21/09 10:00 AM (15 years, 5 months ago) |
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Holy crap that looks like mycellium now
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Stealthgrower
n00b Pwner



Registered: 04/19/09
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Re: Resurrecting dried shrooms on cardboard :) [Re: UpSwell]
#10718004 - 07/21/09 09:55 PM (15 years, 5 months ago) |
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-------------------- Infea said:
You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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nickc
Psyche-dult



Registered: 04/10/09
Posts: 559
Loc: Melbourne, VIC
Last seen: 4 years, 3 months
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Re: Resurrecting dried shrooms on cardboard :) [Re: Stealthgrower]
#10771028 - 07/30/09 09:14 PM (15 years, 5 months ago) |
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How are they looking now? Just tried this process yesterday with about 10 Weraroa, stems and pouches chopped up finely (stems intact) and have done the same method, but keeping it inside in about 15 degrees and very moist in a plastic container.
Looking forward to seeing how your subs have developed!
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Ambient Forest
Beyond the infinite.



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Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: nickc]
#10784401 - 08/02/09 08:16 AM (15 years, 5 months ago) |
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well... it was worth a try... haha
I took these photos last friday (31/7/09) and the mushrooms were all covered in mould, which even i could tell was definitely mould.
    Also i had been a bit slack/lazy and not checked them for a while the cardboard had begun to dry out a bit, but was still damp between the sheets.
So all in all a failed attempt as far as the resurrection of dried shrooms, still had fun and was quite surprised at how resistant the cardboard has been throughout the process as far as mould is concerned. the mould has not gained a hold on any parts of the cardboard, only left mild spore smudges where it was in direct contact but otherwise nothing else.
This proves how good cardboard could be to begin the growth myc if you had some more freshly picked specimens.
Edit: Repairing broken photo links.
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Edited by Ambient Forest (06/14/10 07:18 AM)
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10784508 - 08/02/09 08:52 AM (15 years, 5 months ago) |
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Ok, so ive learnt that dried shrooms are no good to resurrect, but if you have read through some of my earlier posts i showed that i placed a few shroom stubs covered in dirt that looked like they had a bit of dried mycelium in them.
Gave em a go as i had no intention of eating those sandy nugs :P Here are the results.
    Unlike mould shroom mycelium seems to really like fresh cardboard. (notice where one of the stubs have been pulled away when opening)
 The difference from mould and shroom myc, very obvious when you know what you are looking at 
So the project was not a complete failure there is still hope i shall continue the grow; but with a few minor changes.
I have removed all the mouldy bits of card and re positioned the myc stubs on some fresh wet cardboard.
 Also to help keep the card moist i am using a 2L milk bottle with a pin prick in it that should save me from hosing down my grow all the time; yet to see how long one full bottle will drip for.

The plan is to get the myc in to a bucket of pine chips as soon as their ready..... not really sure when they will b ready though...? Anyone know how much growth i should wait for before i transfer?
Edit: Repairing broken photo links.
Edited by Ambient Forest (06/14/10 07:16 AM)
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RogerRabbit
Bans for Pleasure



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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10784536 - 08/02/09 09:01 AM (15 years, 5 months ago) |
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Thanks for the update. You might want to try a layer of straw over the cardboard first and let the mycelium get a foothold in that. You might try a search in advanced mycology using my name to get a technique for recovering dry mushroom tissue. I've pulled it off several times, including with a few tiny dry pieces of tissue sent by a member in AU, which I then grew out, printed the fruits from, and sent him back the fresh prints. There's a thread on it around here somewhere. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Stealthgrower
n00b Pwner



Registered: 04/19/09
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Re: Resurrecting dried shrooms on cardboard :) [Re: RogerRabbit]
#10784578 - 08/02/09 09:12 AM (15 years, 5 months ago) |
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I had similar results in my cardboard trials. I noticed that after a short time, even with transferring, that the cardboard was always either too dry or two wet, and very difficult to maintain steady moisture even in the terrarium I used. After a week or two, the cardboard felt slimy so I tossed it. The mycelium did grow, so I guess it's possible with proper care. This just puts me another step closer to agar.
-------------------- Infea said:
You ever seen that movie Constantine. Well they pass into hell several times. In hell there are melting human bodies, in your tub are melting mushroom bodies. Your tub had passed into mushroom hell.
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ArcanePerception
The only rule isthe golden rule



Registered: 04/30/05
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Re: Resurrecting dried shrooms on cardboard :) [Re: Stealthgrower]
#10785335 - 08/02/09 11:58 AM (15 years, 5 months ago) |
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yup cardboard is great. I have used it before when I got a syringe of Tasmanian from earthstongue that was contaminated I think. Anyways all 12 of my jars contaminated with some rusty color mold (I think it was lipstick). I put some grains on cardboard soaked microwaved and cooled. I got enough clean culture after 2 transfers to knock up 10 jars.
-------------------- Oh, welcome to this world of fools
Of pink champagne and swimming pools
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nickc
Psyche-dult



Registered: 04/10/09
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Last seen: 4 years, 3 months
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All very interesting. I have my attempt at culturing Weraroa NZ a few days old now, have used pieces of fresh flesh (and gills) and a few stubs as well from the bottoms of stems. I am putting in around 1 cup of water every 36hours, making sure it's kept well damp and moist.
No idea how long it will take to grow, my idea was to get at least something started before the season finished and then learn more as I go along.
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karode13
Tāne Mahuta




Registered: 05/19/05
Posts: 15,343
Loc: LV-426
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10789320 - 08/02/09 11:21 PM (15 years, 5 months ago) |
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Good to see that you have had some positive results. It is not rocket science after all..
This is a picture of some mycelium grown from stem butts and a piece of pine, the mycelium is about 3 weeks old

I have layered this cardboard mycelium in a container with holes drilled in the base and placed it indoors so that it can form a good strong layer before adding wood chips.
Close up of mycelium

I give FAE as much as I can and mist with a hand sprayer when needed. These pictures are a week or two old so I will take new ones and probably start a new thread.
~good luck all
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
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Re: Resurrecting dried shrooms on cardboard :) [Re: karode13]
#10790386 - 08/03/09 04:53 AM (15 years, 5 months ago) |
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Fk yeah that myc looks huge! cant wait 4 mine to have some more substantial growth like urs
so at that stage u still waited for more growth before adding it to woodchips...?
have you put them in chips yet? if not can you show me a pic of how much growth you wait for before putting them in chips.
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karode13
Tāne Mahuta




Registered: 05/19/05
Posts: 15,343
Loc: LV-426
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Re: Resurrecting dried shrooms on cardboard :) [Re: Ambient Forest]
#10790493 - 08/03/09 06:01 AM (15 years, 5 months ago) |
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It has two layers, so I just wait until they have joined and things are healthy, then a layer of chips will be placed on top. I will end up making a thread when I can be bothered putting the chips on top, probably in the next few days.
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Ambient Forest
Beyond the infinite.



Registered: 07/16/09
Posts: 544
Loc:
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Re: Resurrecting dried shrooms on cardboard :) [Re: karode13]
#10994619 - 09/03/09 07:42 PM (15 years, 4 months ago) |
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Today i checked the grow i have going, there was still active myc and the 2L milk carton was still keeping the card moist with a minute trickle of water (Not much but still some left in the carton). I have refilled the carton and left it there, will check it every now and then maybe they will survive until next year..... but i doubt it, summer is hot where i live.
I have once again missed the season ;'(
Oh well, until next years hunting :P
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