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Offlinedankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 7 years, 9 months
3 weeks no germination
    #1070357 - 11/20/02 04:19 PM (14 years, 24 days ago)

Can a concentration of to much water in your jars during incubation, halt spore germination? This is the only factor i can come up with, why my jars havent germinated in the last 3 weeks. There are tiny water droplets on the sides of the jars.


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OfflineOn_the_Down-Low
Polyfil-ophile-Say it 3 timesfast.

Registered: 10/15/02
Posts: 401
Last seen: 11 years, 10 months
Re: 3 weeks no germination [Re: dankcrop]
    #1070406 - 11/20/02 04:32 PM (14 years, 24 days ago)

The 'dillo has seen dry jars and soggy jars germ. Could it have been the sprores?Do the jars have any kind of smell?


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OfflineFred Garvin
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Registered: 09/24/02
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Re: 3 weeks no germination [Re: dankcrop]
    #1070408 - 11/20/02 04:32 PM (14 years, 24 days ago)

That amount of condensation sounds normal. There are a number of factors that could cause your spores to fail to germinate. Give some more details on your procedures.



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The above statements are just the incoherent babblings of your friendly neighborhood Cracker!

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Offlinedankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 7 years, 9 months
Re: 3 weeks no germination [Re: Fred Garvin]
    #1070436 - 11/20/02 04:42 PM (14 years, 24 days ago)

Ok here are the facts. I gave the syringe one good shake before innoculation. from the time i innoculated the 1st jar to the 10th was about 15min. But during this 15minutes the syringe was tipped over after each jar. So shouldnt that be adequate enough? the last 3 jars got an extra cc than the other7. But i didnt mark them so i dont know if my 3 colonizing jars are the ones with the extra cc. I dont know its a very complicated situation. All of the enviromental factors are right. i give up


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OfflineFred Garvin
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Re: 3 weeks no germination [Re: dankcrop]
    #1070447 - 11/20/02 04:44 PM (14 years, 24 days ago)

How long after steriliztion did you wait to inoculate?



--------------------


The above statements are just the incoherent babblings of your friendly neighborhood Cracker!

Shur drinkin kils brane sells--but only the week ones!!


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Offlinedankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 7 years, 9 months
Re: 3 weeks no germination [Re: Fred Garvin]
    #1071219 - 11/20/02 08:09 PM (14 years, 24 days ago)

I waited 24 hours. Maybe there wasnt a sufficent spore count in the syringe. Theres a million possibilties.

Do you think when i case my 3 good cakes, i could take some colonized substrate and somehow get it into the failing jars?

I normally would make new substrate but due to security risks i dont really have rescources to sterilize my substrate. To many questions would be asked.


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