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Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars, Hot Plate, Pressure Cooker

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Offlinemaximus_sedaius
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Registered: 10/09/02
Posts: 40
Last seen: 15 years, 1 month
sterilization technique
    #1066489 - 11/19/02 04:24 PM (16 years, 26 days ago)

hi, could someone who has tried this answer..... i made a honey jar and i believe it was uncontaminated but i could be wrong.... anyway i didnt really have time to sterilize a syringe properly so i just filled it with hydrogen peroxide......i squirted some of it into a cup of water emptied the syringe and then took in some of the water i then put some of this water into the jar and sucked up some of the little whitish strands that were floating around...... i was wondering if a)the mycelium in the syringe would be killed from the hydrogen peroxide b)i now have a sterile mycelial water syringe or if i had sterile water now


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OfflineSkikid16
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Registered: 06/27/02
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Re: sterilization technique [Re: maximus_sedaius]
    #1066501 - 11/19/02 04:26 PM (16 years, 26 days ago)

Just hydrogen peroxide will not sterilize your syringe, don't mean to upset you, but most likely your syringe is contaminated, do a test jar to be sure.


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Offlinekykeon
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Re: sterilization technique [Re: maximus_sedaius]
    #1066509 - 11/19/02 04:28 PM (16 years, 26 days ago)

well

H2O2 doesnt kill the mycelium BUT it doesnt kill also a lot of other organisms. Bacteria for example dont die by pouring hydrogen peroxide. They need high temp for long periods. Thats why we use pressure cookers. :smile: If only it was that easy, that we could handle all contams with H2O2...

Kykeon
athens greece 


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Offlinemaximus_sedaius
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Registered: 10/09/02
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Re: sterilization technique [Re: maximus_sedaius]
    #1066602 - 11/19/02 04:58 PM (16 years, 26 days ago)

i was just wondering if an alcohol like isopropanol would do the job of sterility?
would it kill the bacteria ?? and if so would it kill the mycelial too


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OfflineSkikid16
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Re: sterilization technique [Re: maximus_sedaius]
    #1066647 - 11/19/02 05:09 PM (16 years, 26 days ago)

Heat will kill everything, thats why you should use it to sterilize, and yes isopropyl will kill mycelium


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Offlinemaximus_sedaius
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Posts: 40
Last seen: 15 years, 1 month
Re: sterilization technique [Re: Skikid16]
    #1066672 - 11/19/02 05:14 PM (16 years, 26 days ago)

dude you dont have to act like your the king shit of this board......
thx for answering my question next time could you leave your condesending attutide at home i get enough of it at work


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Offlinemaximus_sedaius
newbie
Registered: 10/09/02
Posts: 40
Last seen: 15 years, 1 month
Re: sterilization technique [Re: Skikid16]
    #1066690 - 11/19/02 05:18 PM (16 years, 26 days ago)

ididnt realize your are we there yet comment is in all your posts i thought you were trying to be rude to a noob..... that seems to go around alot


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InvisibleCheeba
the shroomyqueen

Registered: 09/23/02
Posts: 158
Loc: atop my secret mountain
Re: sterilization technique [Re: maximus_sedaius]
    #1066710 - 11/19/02 05:22 PM (16 years, 26 days ago)

lol


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OfflineSkikid16
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Re: sterilization technique [Re: maximus_sedaius]
    #1066727 - 11/19/02 05:27 PM (16 years, 26 days ago)

Hehehehe, that was pretty funny, no I wasn't trying to be rude at all. Good luck man.


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Re-Defeat Bush in '04


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Offlinemaximus_sedaius
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Registered: 10/09/02
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Re: sterilization technique [Re: Skikid16]
    #1066761 - 11/19/02 05:36 PM (16 years, 26 days ago)

its been a long day dude


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OfflineSkikid16
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Re: sterilization technique [Re: maximus_sedaius]
    #1066770 - 11/19/02 05:39 PM (16 years, 26 days ago)

I know how those go. Its cool, like I said, it made me laugh.


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OfflineOn_the_Down-Low
Polyfil-ophile-Say it 3 timesfast.

Registered: 10/15/02
Posts: 401
Last seen: 13 years, 10 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066940 - 11/19/02 06:43 PM (16 years, 26 days ago)

I guess that's what passes for an apology.

I wonder if using bleach and hot water would work?
Pulling up boiling water into a syring and allowing it to cool- and repeating several times-will usually work well.
Shoot a test jar.


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Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars, Hot Plate, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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