Home | Community | Message Board


Avalon Magic Plants
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Pressure Cooker

Jump to first unread post. Pages: 1
Offlinemaximus_sedaius
newbie
Registered: 10/09/02
Posts: 40
Last seen: 14 years, 1 month
sterilization technique
    #1066489 - 11/19/02 04:24 PM (15 years, 1 month ago)

hi, could someone who has tried this answer..... i made a honey jar and i believe it was uncontaminated but i could be wrong.... anyway i didnt really have time to sterilize a syringe properly so i just filled it with hydrogen peroxide......i squirted some of it into a cup of water emptied the syringe and then took in some of the water i then put some of this water into the jar and sucked up some of the little whitish strands that were floating around...... i was wondering if a)the mycelium in the syringe would be killed from the hydrogen peroxide b)i now have a sterile mycelial water syringe or if i had sterile water now


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 12 years, 8 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066501 - 11/19/02 04:26 PM (15 years, 1 month ago)

Just hydrogen peroxide will not sterilize your syringe, don't mean to upset you, but most likely your syringe is contaminated, do a test jar to be sure.


--------------------
Re-Defeat Bush in '04


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 11 years, 2 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066509 - 11/19/02 04:28 PM (15 years, 1 month ago)

well

H2O2 doesnt kill the mycelium BUT it doesnt kill also a lot of other organisms. Bacteria for example dont die by pouring hydrogen peroxide. They need high temp for long periods. Thats why we use pressure cookers. :smile: If only it was that easy, that we could handle all contams with H2O2...

Kykeon
athens greece 


--------------------
The living ghost of Kykeon


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemaximus_sedaius
newbie
Registered: 10/09/02
Posts: 40
Last seen: 14 years, 1 month
Re: sterilization technique [Re: maximus_sedaius]
    #1066602 - 11/19/02 04:58 PM (15 years, 1 month ago)

i was just wondering if an alcohol like isopropanol would do the job of sterility?
would it kill the bacteria ?? and if so would it kill the mycelial too


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 12 years, 8 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066647 - 11/19/02 05:09 PM (15 years, 1 month ago)

Heat will kill everything, thats why you should use it to sterilize, and yes isopropyl will kill mycelium


--------------------
Re-Defeat Bush in '04


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemaximus_sedaius
newbie
Registered: 10/09/02
Posts: 40
Last seen: 14 years, 1 month
Re: sterilization technique [Re: Skikid16]
    #1066672 - 11/19/02 05:14 PM (15 years, 1 month ago)

dude you dont have to act like your the king shit of this board......
thx for answering my question next time could you leave your condesending attutide at home i get enough of it at work


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemaximus_sedaius
newbie
Registered: 10/09/02
Posts: 40
Last seen: 14 years, 1 month
Re: sterilization technique [Re: Skikid16]
    #1066690 - 11/19/02 05:18 PM (15 years, 1 month ago)

ididnt realize your are we there yet comment is in all your posts i thought you were trying to be rude to a noob..... that seems to go around alot


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleCheeba
the shroomyqueen

Registered: 09/23/02
Posts: 158
Loc: atop my secret mountain
Re: sterilization technique [Re: maximus_sedaius]
    #1066710 - 11/19/02 05:22 PM (15 years, 1 month ago)

lol


--------------------
Carpe Diem <*))))><
Theres one sweet whirld out there!!
Now remember, you are unique! Just like everybody else!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 12 years, 8 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066727 - 11/19/02 05:27 PM (15 years, 1 month ago)

Hehehehe, that was pretty funny, no I wasn't trying to be rude at all. Good luck man.


--------------------
Re-Defeat Bush in '04


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemaximus_sedaius
newbie
Registered: 10/09/02
Posts: 40
Last seen: 14 years, 1 month
Re: sterilization technique [Re: Skikid16]
    #1066761 - 11/19/02 05:36 PM (15 years, 1 month ago)

its been a long day dude


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 12 years, 8 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066770 - 11/19/02 05:39 PM (15 years, 1 month ago)

I know how those go. Its cool, like I said, it made me laugh.


--------------------
Re-Defeat Bush in '04


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineOn_the_Down-Low
Polyfil-ophile-Say it 3 timesfast.

Registered: 10/15/02
Posts: 401
Last seen: 12 years, 10 months
Re: sterilization technique [Re: maximus_sedaius]
    #1066940 - 11/19/02 06:43 PM (15 years, 1 month ago)

I guess that's what passes for an apology.

I wonder if using bleach and hot water would work?
Pulling up boiling water into a syring and allowing it to cool- and repeating several times-will usually work well.
Shoot a test jar.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* hydrogen peroxide truth? antimatt3r 5,468 17 12/02/10 02:06 PM
by klen11
* Hydrogen Peroxide growing in non sterile conditions Armageddon 3,060 4 01/18/05 04:42 PM
by Mig
* Hydrogen Peroxide in the Humidity? igore 2,940 2 04/23/03 12:45 PM
by Diploid
* Hydrogen Peroxide?
( 1 2 all )
Leukoism 4,653 26 09/27/04 06:40 PM
by scatmanrav
* OMFG Hydrogen Peroxide Owns! (for being careless and lazy) ShivaMeme 5,579 1 03/18/08 10:36 PM
by Bejeezis
* Hydrogen Peroxide questions... djcontra 1,682 13 06/05/01 05:02 PM
by pl151
* Is Hydrogen Peroxide Underrated, or is it just me???
( 1 2 all )
DocPsilocybin 3,845 21 05/23/12 03:48 AM
by Psilosopherr
* Oxygenation of Liquid Culture by Hydrogen peroxide breakdown Jean-Luc Picard 4,420 12 01/20/09 10:54 PM
by Jonat

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
905 topic views. 14 members, 48 guests and 20 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Edabea
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.005 seconds on 19 queries.