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InvisiblePastywhyteMDiscord
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: camplo]
    #23208298 - 05/09/16 11:38 PM (8 years, 1 month ago)

Quote:

camplo said:
Quote:

Pastywhyte said:
Thanks man! I am technically on a break but I got me some plans to slurry spawn stuff. My Ganoderma neo-japonicum is not as aggressive as lucidum but I want to pull a mono off with it. Thinking some sawdust brf cakes slurried to more sawdust might just gimme the mass nock points I need to spawn that in open air.





I think that there is a formula for this. What ever amount you can use to colonize a quart jar in 3 days...say 30ml per 700ml grain. If you can tally up how much substrate you are spawning to, when you spawn, provide enough liquid in the slurry to reach 30ml per 700ml of substrate. The same effect of colonization should be observed....in the theory.  :shrug:




Yeah provided you are not worried about having a potentialy low spawn ratio. Bulk substrate can hold a lot more water than grains as well. I'm gonna use a bit more slurry than that

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Offlinecamplo
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Pastywhyte]
    #23208872 - 05/10/16 05:37 AM (8 years, 1 month ago)

Quote:

Pastywhyte said:
Quote:

camplo said:
Quote:

Pastywhyte said:
Thanks man! I am technically on a break but I got me some plans to slurry spawn stuff. My Ganoderma neo-japonicum is not as aggressive as lucidum but I want to pull a mono off with it. Thinking some sawdust brf cakes slurried to more sawdust might just gimme the mass nock points I need to spawn that in open air.





I think that there is a formula for this. What ever amount you can use to colonize a quart jar in 3 days...say 30ml per 700ml grain. If you can tally up how much substrate you are spawning to, when you spawn, provide enough liquid in the slurry to reach 30ml per 700ml of substrate. The same effect of colonization should be observed....in the theory.  :shrug:




Yeah provided you are not worried about having a potentialy low spawn ratio. Bulk substrate can hold a lot more water than grains as well. I'm gonna use a bit more slurry than that





You should be able to use any spawn ratio you want. So lets say I got 4 quarts of spawn, and 4 quarts of substrate.
  4grain qts = 4*700
  4Sub qts = 4*946
+____________________
6,584/700=9.40*30=282.171

So use 282.171ml of water to slurry one jar and mix it all up. If you are going to use more water than that, even better? I would be worried about makeing it too wet.


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InvisiblePastywhyteMDiscord
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: camplo]
    #23209013 - 05/10/16 06:41 AM (8 years, 1 month ago)

Sweet. I'm all over that. But why are you calculating for spawn in that? Spawn should already be colonized.

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Pastywhyte]
    #23209658 - 05/10/16 10:17 AM (8 years, 1 month ago)

so any opinions on some sterilized water being viable after 5 days of sitting uninoculated in my blender bottles with sfd's for GE ?


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Offlinecamplo
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #23211552 - 05/10/16 07:06 PM (8 years, 1 month ago)

Quote:

Pastywhyte said:
Sweet. I'm all over that. But why are you calculating for spawn in that? Spawn should already be colonized.



I have no idea...lol, thats a different formula.. :heytherebadboy:
Quote:

tombosley8 said:
so any opinions on some sterilized water being viable after 5 days of sitting uninoculated in my blender bottles with sfd's for GE ?



I always get scared and re-pc stuff like that anyway.


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InvisibleGrey
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #23213338 - 05/11/16 07:45 AM (8 years, 1 month ago)

Quote:

tombosley8 said:
so any opinions on some sterilized water being viable after 5 days of sitting uninoculated in my blender bottles with sfd's for GE ?



If it's sterilized, it should be fine. If it bothers you that much, re-pc.


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Offlinecamplo
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Grey]
    #23227589 - 05/14/16 09:08 PM (8 years, 1 month ago)

Well I put my money where my mouth was and tried it out. ~3 quarts of grain and ~2 quarts of coir/coffee/straw/wormcastings/rabbitmanure .5*946=4730/700 = 6.75 = 202. So I took 200ml of colonized millet, added 200ml of water and did a wetg2g, or rather in this case, a wet grain to grain/substrate. The substrate is now about fully colonized and its just upon day 3.

Eats I thought this would interest you, wetG2G is slurry's little brudah...or adopted brother...cousin... :cheerup:


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:three:

Edited by camplo (05/15/16 04:46 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: camplo]
    #23230583 - 05/15/16 04:09 PM (8 years, 1 month ago)

yeah id agree. if the water is sterile and sealed it should be fine. ill take a look when i have a little more time on my hands.


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Offlinecamplo
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23241666 - 05/18/16 11:33 AM (8 years, 1 month ago)

Since its in your I wanted to make sure and report back results. My results are maybe slightly skewed. I pasteurized my mix that includes horse manure for 90 minutes at 140. Either that wasn't enough or the substrate was too wet or a little of both. Smelled like apples the next day after spawn.
I am currently giving it another go but this time I removed 200ml from the hydrated substrate so when I add the 200ml during spawn run, there will be no excess.
My sterilized substrate did colonize... So why not just raise the bar and suggest to prep a substrate to receive any additional water that is added to a substrate. Not always necessary if using small amounts of water but is optimal if water content is adjusted to receive the inoculate when using large amounts, which all makes perfect sense.




I've always been suspicious if whether or not you could use slurry to colonize a grain/substrate or even grains that have been sterilized, with open air inoculation. It would show the limits of the procedure. I knocked this guy up in front of the flow hood. I'm suprised that with a opening so large that I could do so and remain sterile, or is it just the efficacy of the inoculate...


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Edited by camplo (05/18/16 11:38 AM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: camplo]
    #23281501 - 05/28/16 02:38 PM (8 years, 1 month ago)

Hey i wanted to try this method but with a few aluminum pans as "mini mono tub tek " I was wondering if i could wash my grains and skip the mason jar step and just pour slurry into aluminum pans filled  with rye grain to colonize ?
Does the success rate sound low , has anyone done this , i think eats attempts this above , but im not sure if its recommened with a decent yield .
Thanks .

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: som776]
    #23326145 - 06/09/16 02:53 PM (8 years, 22 days ago)

How long does LI or liquid spawn stay good for? Can I use some of it and then save some for later or should it be used basically as soon as its created? Would be nice to be able to save some for when I need jars ready in like 3-5 days. I used LI on some Reishi grain jars and they were done in like 4 days. Wish I had used some grain slurry or LI to inoculate the sawdust bags because this stuff looked like it moved so fast in the grain jars and now I've been waiting for what seems like forever for the grains to colonize the sawdust.

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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
    #23562562 - 08/21/16 10:20 AM (7 years, 10 months ago)

Quote:

The only obstervation that I believe would cause an issue is if you use larger size grain like Rye berry or Oats. The size of the inner uncolonized grain ends up being too much exposure for contams to set in unless you don't blend the grain as much. The smaller millet or wbs grain doesn't quite get pulverized as much so the grain itself isn't as exposed.



Due to this, does anybody blend up larger grains without any water and let them recover. Maybe it is already mentioned in this thread. I had colonised wheat which I stuck in a blender and let recolonise, I did this a second time with the aim of being like small grains like millet and having a large amount of myc going into bulk. I also guess it makes the nutrients more available for the first couple of flushes, which might be all some bother to do.

I am planning on trying this with popcorn for sclerotia, I heard popcorn is terrible for sclerotia and think it could be due to the hard outer casing not allowing it to penetrate.

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: blackout]
    #23617699 - 09/06/16 10:24 AM (7 years, 9 months ago)

Hey eats . I was just wondering if the yields of these grows where all dry or wet ?

Thanks .

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: som776]
    #23647541 - 09/15/16 01:39 PM (7 years, 9 months ago)

These are some blended cube popcorn jars that I was talking about in my previous post.

this crap photo is colonised popcorn which was 100g(100ml) water added to 100g popcorn


same below jar shaken (grains are dark as they were heated at 97C for several days)


same jar after being in a blender and the blended paste scraped back into the same jar. It is laid on its side to get a good surface area for myc to grow.


the same jar 16 days later, after about 3-4 days it was 100% colonised again.


inside that same jar. This has since been added to coir with some gypsum.


This popcorn below was prepared much drier, 71g (71ml) water per 100g popcorn. Before blending it looked the same as the other jar on the right, both were consolidating for several weeks.

The texture of this drier blended jar seemed a lot better than the other which was more like paste, this one is still shakeable and airy. It grew a bit in volume. Having drier popcorn allows you fill the jars a little more than usual as they are like little hard nuts or stones, they break up very easily. I poured out about 1/5th of the jar and tried to chop it up with a kitchen knife, like a chef chopping herbs. These went in with the mixture as I wanted it to be shakeable and I was also concerned the myc may be overblended.

Popcorn is considered a poor grain by many, its hard to hydrate, expensive, is large so has less myc due to less surface area per jar, and it performs poorly for some. By blending it you should get far more myc, and I beieve the poor performance may be since the hard shell makes it harder for myc to readily get nutrients from it. By blending I think it may readily give up nutrients. Usually we do not want burst grains as the starchiness is prone to contams, these grains were well colonized and I expect they will recolonise very quickly. I did not want to make a slurry or mix with bulk as this would dilute the relatively small amount of myc too much for my liking, and the newly exposed innards might contam.

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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
    #23754928 - 10/20/16 01:41 PM (7 years, 8 months ago)

Hi eat

Is still your go to method of expanding spawn?

Im going to try a little expansion in the next few days.

Wish me luck

Regards

E

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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: Edmunter]
    #23806407 - 11/06/16 12:35 PM (7 years, 7 months ago)

dry weight.

hey guys. i haven't been growing in a while. if i were to do big expansion then id say yes on the go to for expansion. but ive been messing with cornmeal plate agar blends recently.

and i want to try brown rice paste liquid cultures soon.

good luck.

good info, thanks blackout.


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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
    #24028379 - 01/21/17 02:48 PM (7 years, 5 months ago)

I just tried this with a colonized half pint wbs spawn (reishi) onto some pastuerized shredded paper. I threw half pint of colonized grain along with a quarter pint of water into the blender. The slurry was really muddy but I used it anyway.

Muddy slurry works just as well?

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Swervin]
    #24096263 - 02/16/17 07:10 PM (7 years, 4 months ago)

If your blending up grains I'd use it immediately.

Slurry is muddy.


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Edited by eatyualive (02/16/17 07:10 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #24096339 - 02/16/17 07:38 PM (7 years, 4 months ago)

Slurry is the bomb. Mud is perfect

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tump]
    #24662385 - 09/26/17 07:06 PM (6 years, 8 months ago)

How many pf cakes slurried directly to coir is recommended for a good yield in a standard mono?


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Edited by JHOVA (09/26/17 07:07 PM)

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