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OfflineMyko Fanatikos
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23023393 - 03/19/16 02:08 PM (8 years, 3 months ago)



UPDATE:

I did switch lids because I didn't think just cracking then slightly would work. I really didn't know any better until I had already switched them, but here's 3 days later and they are fully colonized.

I'll be spawning to bulk monday. This will be my first attempt, wish me luck.


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Edited by Myko Fanatikos (03/19/16 02:49 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Myko Fanatikos]
    #23024208 - 03/19/16 06:36 PM (8 years, 3 months ago)

ah so GE was the issue. good to know. i never much liked polyfil as a filter for lids. those look great. its amazing what a small tweak can do to change the game.

myko,

what are you planning on spawning as your bulk substrate?


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Edited by eatyualive (03/19/16 06:41 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23024220 - 03/19/16 06:40 PM (8 years, 3 months ago)

som, when you get the time, upgrade your lid filters to either synthetic filter disk cutouts or easy felt. i think it will make a big difference along with lowering your inoculation volume per jar.


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OfflineMyko Fanatikos
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23024543 - 03/19/16 08:24 PM (8 years, 3 months ago)

Yeah GE was definitely the issue, they stalled out on day 2 and took off like a bat out of hell after I switched lids.

I plan on using coir, verm and gypsum as my bulk substrate and I only have 10 wbs jars so i'm using 5 in one tub and 4 in the other. I plan on saving 1 as a master for G2G so I can continue experimenting.

I looked I to the bucket tek and was planning on using it but seeing as I am only running 2 tubs I don't want to take chances, so I'm going to use Franks pasteurization tek and hope for the best.

I had to spend a little extra for the thermometer but I'd rather spend a little and do things right so I get a worth while outcome.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Myko Fanatikos]
    #23024560 - 03/19/16 08:29 PM (8 years, 3 months ago)

the GE factor could be some of the issues people have been having problems with in the past. ive always used the sfd filters or whatman ports and never had an issue. sometimes people use poked holes with polyfil or other filters. personally, i think a good filter makes all the difference on your lids. probably one reason i never have many issues.


you could also take one spoon full of the saved quart to use as slurry for more jars as well.

im not familiar with frank's pasteurization tek. but if it involves jars and cooking things while paying attention. thats alot of work for me. i like doing as little as possible.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23024578 - 03/19/16 08:33 PM (8 years, 3 months ago)

Best GE I ever had was an unmodified lid cracked a half inch. Great GE but you can't handle them much and great care must be taken if you shake to speed colonization.

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Pastywhyte]
    #23024597 - 03/19/16 08:39 PM (8 years, 3 months ago)

yeah we were talking about that a few posts back pasty. i believe someone had forgotten to cut holes in the lids. so i was saying to crack them. i had my whatman ports die after about 10 years of use. i cracked my lids and they finished colonizing after stalling out. i made sure to tighten the lid before shaking. then loosen again during colonization. i would try the non modified lids but all the lids i own have a number of holes and filters in them :lol:


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OfflineMyko Fanatikos
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23024955 - 03/19/16 10:54 PM (8 years, 3 months ago)

Hah I was the one who was rushed after invitro pinning and forgot to put holes in the lids.

I use micropore tape over my lid holes mainly because I am very new to all this and haven't got around to making permanent lids with filters or silicone.

Don't cou t me out though I've done enough research for 3 people in the past month to be 100% familiarized with the teks I'm following. The most important line in the pf tek for me was

FOLLOW YOUR TEK 100%.

I don't really want to babysit quarts of sub at all, easy peasy is the way to go, but I also want to be sure that I'm not going to get contams with my first bulk grow and the little bit of extra time I'll spend is worthy the piece of mind I'll get from knowing I properly pasteurize my sub.

If there's an easier way that can give me that piece of mind, then please lease me in the right direction.

After I had already switched out lids was when I found d a thread pasty had posted in saying he had used cracked lids in the past for GE but the deed was done by then.


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OfflineMyko Fanatikos
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Myko Fanatikos]
    #23024968 - 03/19/16 10:58 PM (8 years, 3 months ago)

Eatyualive, how many jars could I spread a spoonful of colonized wbs, via slurry, out to?

I haven't perfected my sterile procedure but I do a pretty good job for a newbie I think.

I was planning to use G2G but I really like how hands on the slurry is and I would like to continue to experiment with it.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Myko Fanatikos]
    #23025115 - 03/20/16 12:23 AM (8 years, 3 months ago)

20. using a half pint of water. maybe even more.

id highly suggest g2g. you should at least try it to know what its all about.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23025721 - 03/20/16 09:21 AM (8 years, 3 months ago)

Quote:

eatyualive said:
20. using a half pint of water. maybe even more.

id highly suggest g2g. you should at least try it to know what its all about.




:whathesaid: G2G is actually pretty effective. Depending on your actual needs it will provide a lot of expansion and if not stretched too far can still colonize very quick. I like to use millet for grain masters for the high nock points.

One thing about G2G that people miss is that it recovers very fast, especially if you do not let it sit and get overgrown. Recovery in 12 hours is typical for me.

Sorry eats, not trying to derail yer thread.

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Pastywhyte]
    #23027133 - 03/20/16 05:08 PM (8 years, 3 months ago)

what, you didn't derail anything.:thumbup:

and im not too snobby about shit like that lol. come one, come all.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23027183 - 03/20/16 05:22 PM (8 years, 3 months ago)

Thanks man! I am technically on a break but I got me some plans to slurry spawn stuff. My Ganoderma neo-japonicum is not as aggressive as lucidum but I want to pull a mono off with it. Thinking some sawdust brf cakes slurried to more sawdust might just gimme the mass nock points I need to spawn that in open air.

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Pastywhyte]
    #23027636 - 03/20/16 07:19 PM (8 years, 3 months ago)

Hey Eat I'm having trouble finding your reply from way back about how much lc you inoculate your dryer cakes with? Also, how much do you think is too much or is there a point where your just wasting it?


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Edited by tombosley8 (03/20/16 07:20 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #23031351 - 03/21/16 08:02 PM (8 years, 3 months ago)

oh do you mean pf cakes?

what i would do is inoculate 5ccs per pf jar. so one whole syringe into 2 pf jars. that is with clone tissue. and it colonizes in 3-5 days. so its as fast as agar. but im also using a large volume of clone material. id do that with 9er tek. or you could take a plate and suck up a syringe then inoculate. if you have an LI syringe stored in the fridge, that is a perfect candidate.

do you mean "how much is too much when inoculating into a pf jar?"

if your inoculating into a dryer mix pf jar. you can do a full syringe with good results. i do have two 1/4" holes in my lids. and i use 4-6 inoculationg points. ill even inject some into the middle. so the key is to put the syringe tip at the edge of the glass and drip your inoculant to the bottom of the pf jar if you use the tall pf jars. i did 1 full syringe into 1 pf jar a few times and it worked just fine. actually faster than a half syringe of cloned mycelia. but, i would not go any higher than that. that is probably overkill but im all about trying as much as i can.

Quote:

STEM TISSUE GROWING IN PF JARS

ok so im going to illustrate a stem clone taken from Ape strain. it was done using 9er tek and the illustration that was posted in this thread. I did 3 syringes. One syringe was done in 2 pf jars. The other two syringes were done in 4 pf jars each. You can see below how the stem tissue jars that were inoculated with 1 syringe per 2 pf jars is going to explode tomorrow.

Pf jar lids and recipe for pf mix

before you all flame me for having foil on my lids. its not stopping ge and its not causing water buildup in the verm barrier or any type of stalling. i make my mix drier than the normal pf mix so the cakes crumble into small pieces for P2G 123(PF to grain transfer) or Slurry. the lids are illustrated below.



Quote:

my recipe for 24 pf jars
12 cups verm
6 cups brf
5-5.5 cups water. i use a half cup to a full cup less so the pf jars are a little drier. i intentionally do this for liquid culture injections.

regular 24 pf jar recipe
12 cups verm
6 cups brf
6 cups water

this post shows some close ups of my mix. and a description of how i add that last bit of water to the mix.

http://www.shroomery.org/forums/showflat.php/Number/20300997#20300997

here is another post i made with some better close ups of the wet mix after i blend it with a hand mixer.

http://www.shroomery.org/forums/showflat.php/Number/20428297#20428297

ill then use a syringe full of lc and inject one whole or a half syringe into 1 pf jar. this grows very fast with liquid cultures in a few days and then i can g2g that or blend it up and make a ton of jars with a slurry. you may even find that if your going to add a whole syringe to the pf jar, you may want to add 1 cup less than the 6 cups total water for the 24 pf jars. just divide the formula by 24 to get the individual jar ratio.

but if you intend to try the slurry. id inject your liquid inoculant with a sterile syringe into a pf jar. i usually use a sterile syringe and suck up the liquid stem tissue into it, then inject into a pf jar. this smaller volume of inoculant in one pf jars allows ou to test cultures rather easily. also, if your only injecting 2 pf jars. your not contaminating 10 quarts if your made some errors during the procedure. so pf jars for me are also a way of testing if something is clean or not.



INOCULATION TIP FOR TALL REGULAR MOUTH 1/2 PINTS: Once your needle is in the jar point it towards the edge of the glass and trickle down inoculum to the bottom of the jars-"Original Pf Tek". This way you colonize the jars from the bottom up. You can observe this below. You can use wide mouth or regular mouth jars. They work exactly the same.
Sequence of stem clone growth in pf jars and grain masters. the jar on the far left in the pf jar pictures was injected with 1-10 cc syringe in the jar. it colonized in 4 days.






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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23047988 - 03/26/16 12:26 PM (8 years, 3 months ago)

I accidently left my cakes sterilizing at 17 psi for a little over two hours. Will they be burnt or overcooked to where mycelium won't grow(or grow slower than normal)???


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Edited by tombosley8 (03/26/16 12:27 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #23048020 - 03/26/16 12:37 PM (8 years, 3 months ago)

they may be ok. if you look at them, and they are burnt looking. its probably overkill. if they are a little brown they should be ok.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #23048032 - 03/26/16 12:43 PM (8 years, 3 months ago)

They should be alright as long as the moisture content was right. Some parts at the bottom of the jar may be compact, but shouldn't hinder what your aiming for.

Edit: beat me Eat.

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #23207559 - 05/09/16 08:09 PM (8 years, 1 month ago)

Hey thanks again I definitely burnt those...


Anyway, I pc'd a few blender bottles a few days ago (I think about four now) And am wondering if there would be a problem with using them as I made GE holes with thick felt sfd's over them?

Shouldn't sterilized water stay sterile for a long time?

Or would you recommend re sterilizing to ensure that the dust accumulation on the outside of the container will not compromise my sterile tek?

Since I will be wiping them down with iso the dust accumulation shouldn't be a problem, right?


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Pastywhyte]
    #23208279 - 05/09/16 11:31 PM (8 years, 1 month ago)

Quote:

Pastywhyte said:
Thanks man! I am technically on a break but I got me some plans to slurry spawn stuff. My Ganoderma neo-japonicum is not as aggressive as lucidum but I want to pull a mono off with it. Thinking some sawdust brf cakes slurried to more sawdust might just gimme the mass nock points I need to spawn that in open air.





I think that there is a formula for this. What ever amount you can use to colonize a quart jar in 3 days...say 30ml per 700ml grain. If you can tally up how much substrate you are spawning to, when you spawn, provide enough liquid in the slurry to reach 30ml per 700ml of substrate. The same effect of colonization should be observed....in the theory.  :shrug:


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