|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
#22978094 - 03/05/16 11:50 PM (8 years, 3 months ago) |
|
|
i think those blades will work. although the regular oster are high power enough. this one might actually work even better. but you might only blend for 1-2 seconds.
|
Shroomin 101
KISS

Registered: 08/30/15
Posts: 120
Loc: Around The Corner
Last seen: 4 years, 30 days
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#22983803 - 03/07/16 07:17 PM (8 years, 3 months ago) |
|
|
I bought a few extra black blender bases that were aftermarket (big mistake), they would not fit. I was stupid not to try them before using in a real clean setting. I had to stop what I was doing and go into the garage to retrieve channel locks, to loosen. I was forced to use regular jar bands. The jar bands actually worked great. Only thing is you have to keep the blinding jar 100% vertical, without any pivoting action going on ( easy to audibly hear ).
--------------------

|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Shroomin 101]
#22990790 - 03/09/16 10:23 PM (8 years, 3 months ago) |
|
|
This was probably asked somewhere in the last 78 pages but I'm going to indulge myself and ask again now.
I have 1 qt of colonized wbs that I intend to slurry and colonize 20-30 jars. Will I be able to slurry the whole quart jar worth of grain (~3/4 jar) if my blender jar is a small mouthed quart jar? Also, what is the consitency I'm looking for? Pretty muddy I'm imagining.
Next question - Given that a quart of slurry can noc up way more than 20-30 jars, I was thinking I'd increase the volume of slurry for each jar (eg 1 Tbs --> 2 or 3 Tbs per jar). Is this recommended? Will it mess with moisture content in the jars?
Props to Eat and everyone participating in this thread....very excited about this tek!!
|
camplo
Freedom!


Registered: 02/10/08
Posts: 1,675
Last seen: 2 years, 9 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: trravis]
#22991092 - 03/10/16 12:27 AM (8 years, 3 months ago) |
|
|
Quote:
trravis said: This was probably asked somewhere in the last 78 pages but I'm going to indulge myself and ask again now.
I have 1 qt of colonized wbs that I intend to slurry and colonize 20-30 jars. Will I be able to slurry the whole quart jar worth of grain (~3/4 jar) if my blender jar is a small mouthed quart jar? Also, what is the consitency I'm looking for? Pretty muddy I'm imagining.
Next question - Given that a quart of slurry can noc up way more than 20-30 jars, I was thinking I'd increase the volume of slurry for each jar (eg 1 Tbs --> 2 or 3 Tbs per jar). Is this recommended? Will it mess with moisture content in the jars?
Props to Eat and everyone participating in this thread....very excited about this tek!!
2:1 grain to water is the thickest you'd likely want to go. As for the amount to use, it depends on how extreme or conservative you are but 15ml should be enough to finish a jar within 5 days from what I hear.
--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: camplo]
#22994167 - 03/10/16 09:10 PM (8 years, 3 months ago) |
|
|
Quote:
This was probably asked somewhere in the last 78 pages but I'm going to indulge myself and ask again now.
I have 1 qt of colonized wbs that I intend to slurry and colonize 20-30 jars. Will I be able to slurry the whole quart jar worth of grain (~3/4 jar) if my blender jar is a small mouthed quart jar? Also, what is the consitency I'm looking for? Pretty muddy I'm imagining.
Next question - Given that a quart of slurry can noc up way more than 20-30 jars, I was thinking I'd increase the volume of slurry for each jar (eg 1 Tbs --> 2 or 3 Tbs per jar). Is this recommended? Will it mess with moisture content in the jars?
Props to Eat and everyone participating in this thread....very excited about this tek!!
for blending the quart slurries, a regular mouth quart jar works with the 2:1 grain to water ratio. surprisingly it fits. the grain actually breaks down rather easily into a smaller volume once blended.
this is a 2:1 grain to water ratio in a regular mouth quart jar. 1 quart grain to 1 pint water. you want it to look like mycelial soup. just like the picture. the soupier the better.
 
i seriously sometimes just pour straight from the jar to inoculate. no fancy lids or anything. but the lids make the pour more even. and if your scared, its always a good safety to have a nice lid. after a while your tek will get so good, you won't need anything fancy. i always poured straight from the jar with the metal lid on for years. it works just as well. but the jars that have the lid/blender are the easiest and make the most efficient use of time. especially when you wanna do 50 plus quarts. it takes less than an hour to do 100 quarts with the tribal container type builds.
i only use regular mouth quarts, pints, half pints for my blending jars. so yes it will work. you can simply use a sterile spoon to remove the grain and dump it in the blender jar. or you can shake the jar and then just transfer it to the blending jar like a g2g. you probably won't need the entire quart jar of grain to colonize all 30 quarts. you could easily use half that quart. 1 pint to 1/2 pint water. blend it in a regular mouth quart jar with a blender assembly. i think you may be ok using he 2 tablespoons. just make sure to shake the hell out of the jar for about 2 minutes per jar. take a break if you need to in between each jar. shaking upwards of 30 in a row for 1-2 minutes each will blast your forearms.
like this. 2:1 pint of grain to 1/2 pint of water.
i shake up the master grain jar. or you can break it up with a sterile spoon. then transfer to the blending jar. blend, then bring back into the SAB and inoculate all your receiving quarts. (i setup up all the quarts prior to all of this work. or you can do it clean and do the blend with a clean SAB and then put your receiving jars in. personally i like to put everything in my setup one time. so i can do it all in an efficient way. i wipe down everything with alc first then put it in the SAB or shmuvbox. do all my blend and transfer. then do all my pours. so its step 1, 2, 3.
     
these grain slurries below are more like a Grain Liquid Culture. i only used about a spoonful of grain to a larger volume of water. it was the opposite ratio. 2:1 water to grain. this works kind of like munchauzens blenderless post. you simply agitate the mycelia off of the grain/agar plate ect and have a diluted mycelial liquid inoculant. it works really well and will colonize a larger number of quarts. but it does take about 9 days to fully colonize. where the soupy 2:1 grain to water blends take about 3-5 days.
 
you could get away with only pouring out the liquid in the jars above. but it doesn't hurt to pour out that grain into the receiving jars either.
Edited by eatyualive (03/10/16 09:39 PM)
|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#22995467 - 03/11/16 09:45 AM (8 years, 3 months ago) |
|
|
Eat- Thank you so much for the time you put into helping me out!!
IT's perfect timing, I'm just getting everything set up right now so reading this as a quick refreshed before heading in couldn't have been better.
You are like the patron saint of slurry.....St. Slurry 
Wish me luck!
|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: trravis]
#22999097 - 03/12/16 10:54 AM (8 years, 3 months ago) |
|
|
It was a tough start to my slurry campaign....
First problem was not allowing enough time for the PC'd blender jar to cool down. I tried to help the process along with an ice water bath and ended up cracking the jar....derrrrr
Anyway, I've got a question here. How much blending is too much?
I don't think I PC'd the blender jar with enough water, so it took quite a bit of blending to get the grains slurried. My slurry mixture was very very soupy, probably more on the sludgy spectrum TBH.
Have I pulverized those poor, innocent mycelium? Or do you guys think they might be able to recover?
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: trravis]
#22999363 - 03/12/16 12:34 PM (8 years, 3 months ago) |
|
|
yeah. be patient. let your pc cool before removing anything from it. once cool, you can place them directly from the pc into your clean work area.
for grain its like 20-30 seconds. for pf jars, its about the same. LI is only a few seconds.
great, sludgy, soupy is perfect. the reason is, it sticks to the outside of the grain easier and doesn't slide down the grain. so in the end, it actually creates more inoculation points and colonizes faster. the sludge is a perfect description of what you want. i think you will be fine.
|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#23000230 - 03/12/16 05:15 PM (8 years, 3 months ago) |
|
|
Ok, ok, ok......ye of little faith, right?
I've got little points of fuzz appearing on about half the jars. It's very cool to all those innoc points popping up and to see the jar beginning to colonize from many points at once.
So, more questions!! (I hope I'm not annoying here....quest for knowledge and discourse as a public service, right?)
Anywho, what's your guys experience with contams and slurry in general? More specifically, I'm thinking about slurry hiding a contam. In short, does the myc have so much of a head start that it can rush and colonize most of the jar before a contam can show itself? With a normal syringe or even g2g innoc, a contam has the chance to win out against the myc or at least slow it down....all things that can give the cultivator a chance to notice that something might be off with the jar. Does that make sense?
Any thoughts, experiences, etc on the matter greatly appreciated
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: trravis]
#23000720 - 03/12/16 07:30 PM (8 years, 3 months ago) |
|
|
yeah you should see alot of growth by the end of the 2nd day and then by day 3 its almost done if not done.
never annoying i like to help.
well if there is a contam you can't see your just spreading it tenfold with liquids. but if there is a contam you can't see with g2g or any other method of transfer its the same thing. with slurry you will know alot quicker if its contaminated so to me, the indicator that your fucked is faster. instead of waiting 2 weeks for something to colonize that is possibly contaminated. your waiting less than a week. so you will know faster.
you want to ensure a clean culture before expanding any type of culture. that is a given or common knowledge in cultivation.
but my experience with slurry is this. ive done hundreds of batches of slurry jars since 2008. i had one large batch contam in which i had used a questionable culture off the bat. that was my big mistake. i was trying to revive this old culture i had and it didn't work out. but i also knew up front i was willing to take that risk.
so thats the only time ive personally had a batch of jars tank out. it stalled and didn't colonize any further than about halfway. where most people do have problems is that they are introducing contamination by improper technique. or the culture wasn't clean to begin with.
discoloration and anything other than fluffy white is not a good sign. also, if it takes more than a week or stalls out, then there might be something wrong.
the speed does help. winning the speed battle is definitely a plus.
post some pictures if you have any.
|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#23001287 - 03/12/16 09:56 PM (8 years, 3 months ago) |
|
|

Dunno if you can make out the white fuzzes....I was mostly concerned that I destroyed all my innoc material, so, as long as they're alive in their I'm happy. I lost my very first batch of innoc'd WBS jars because I didn't wait for those to cool down from the PC either (they patience is a virtue in this hobby?!?!)
Anywho, something tells me you've spent some time staring at pictures of other people's mushroom's lifespan pictures on a computer monitor. It's a skill I'm getting better and better at the more I hang around this place.
Since you insist I'm not a bother, do you mind if I pick your brain a bit?
This is the start of my second project and I'm quickly learning the importance of controlling contams in this hobby. I lost about 50% of my first project to contams that I suspect were present in a MS syringe. I've read some agar teks, looked over the 9er tek briefly, but I gotta confess I'm still a little confused about the process of isolation)?)/cloning(?)....in effect, trying to get a clean, preferred genetic sample.
I currently have basically one strain fruiting with some nice fruits. Do I clone the best growers? Do I wait until I can hit the marketplace to try and get access to some already isolated strains? I guess I'm a little sour on the prospect of ordering more MS syringes....part of controlling this process seems to be bringing material and process "in house".
Any thoughts? And again, can't say thank you enough for your help
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: trravis]
#23001321 - 03/12/16 10:07 PM (8 years, 3 months ago) |
|
|
yep that looks good. it has begun.
hey check this thread out. it will help you out. clone clustering fruits and you can't go wrong. some people clone different ways. i generally cut inner stem tissue near the base and place it on agar, or blend it up and liquid inoculate it right then and there. this requires setting up all your pf jars or grain jars prior to taking the clones. or if you put it on a plate. you would want all that setup prior to taking the cut.
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=18430998&fpart=&PHPSESSID=
i don't know about the isolated genetics. its definitely worth it to get good genetics off of good people that have worked with the culture and taken prints off good genetics. get accustomed to the board, make some friends and you will find good genetics. once you have some prints for trade. see if someone else has a trade list. politely ask and follow board policies on trades. but you may want to steer clear of pming begging people. if you see someone has a trade list in their journal they may be up for a trade.
the market place sometimes has free giveaways or trades. i have one currently. get them while they last.
https://www.shroomery.org/forums/showflat.php/Number/22999349#22999349
|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#23001474 - 03/12/16 11:03 PM (8 years, 3 months ago) |
|
|
I definitely get what you're saying about speed. so much of this process seems to occur on a delay so being able accelerate steps is a huge help with both efficacy and recognizing and containing a problem.
Wow- thanks for the link. I'm about 30% in and this info is fantastic.
Thanks for the tip on begging. I'm only 2 months into the board so I can't visit the marketplace yet, but I also see the constant influx of new members that seem eager to jump to the front of line instead of getting some reading done and spending time on technique. For now, I'm enjoying paying attention to my project, trying to gather knowledge, and trying out new teks (including slurry!)
|
som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 5 months
|
Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#23001750 - 03/13/16 03:01 AM (8 years, 3 months ago) |
|
|
Great tek
|
som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 5 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: som776]
#23004767 - 03/14/16 01:40 AM (8 years, 3 months ago) |
|
|
Hi , not sure what the rules are for inquring on an open post , but I was hoping to get some help from the pros. It seems that when I attempt the slurry tek my quart jars ,which are 2/3rds full always seem to stall at about 90 % , and if if I load my jars with less rye grain , the bottom of the jar is always really wet and mushy . I'm thinking it might be my grain prep . I rinse the grains many times . Then let air dry , after there dry ( paper towel test shows no moisture at all ) Then I load jars with grain , no water in the jars . Is this wrong and should I be adding water to the jars ? After there loaded I pc for 90 min at 15 psi , with towel under jars so they are not in direct contact with bottom of pc. Does anyone have a clue as to why they turn so mushy at bottom?
Thanks . Appreciate any help .
|
som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 5 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: som776]
#23004771 - 03/14/16 01:46 AM (8 years, 3 months ago) |
|
|
Sorry. These are pics for the post above . 
|
Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: som776]
#23006869 - 03/14/16 06:29 PM (8 years, 3 months ago) |
|
|
Are you just rinsing the grain, or do you soak/boil too hydrate them?
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
|
Myko Fanatikos
Rando



Registered: 02/18/16
Posts: 283
Loc: Earth
Last seen: 1 year, 3 months
|
Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#23007521 - 03/14/16 09:29 PM (8 years, 3 months ago) |
|
|
WARNING FOR USING THIS TEK: NOT FOR NEWBIES
"NEWBIE ATTEMPT AT A SLURRY" I haven't completed my first grow yet. My pf cakes are in the terrarium now. I made 8 cakes for my first attempt and came across this thread after weeks of research. I used tvcasualtys original recipe with 200ml of water but I added a whole of cake to cut back on waste, but it also made my.SLURRY like mud. I took a page from this thread and poured from jar to jar in my SAB without a spout and shook each jar for 2min a pirce. Here's my 48 hour pictures. It's colonizi g from the middle for the most part I think but all seems well and no contam. Wish me luck.  
--------------------

|
trravis
Stranger


Registered: 12/28/15
Posts: 63
Last seen: 8 years, 2 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: som776]
#23008592 - 03/15/16 07:39 AM (8 years, 3 months ago) |
|
|
Quote:
som776 said: Sorry. These are pics for the post above . 

I'm no expert, but you might wanna take those pics over to the contam forum to see if they can give you an opinion. I've seen jars with bacterial contams look like that.
Again, I'm no expert and it could just be how the picture was taken. But, a second opinion is never a bad thing
|
som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 5 months
|
Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: trravis]
#23009867 - 03/15/16 03:11 PM (8 years, 3 months ago) |
|
|
Thanks for the help guys and yes I boil for 10 minutes before adding gypsum for the soak .
|
|