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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22123342 - 08/21/15 04:29 PM (8 years, 10 months ago) |
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update below. so the slurry not only beat the LI but it also fully colonized the jars before most of the LI were complete. I dug in the back and pulled out a few jars of both the LI and Slurry jars up to the front. a few LI in the back weren't all the way done.
Quote:
eatyualive said:
SLURRY VS LIQUID INOCULANT
The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.
    
Here are some of the LI and Slurry jars that were in the back pulled to the front. As you can see, the LI jars are not 100%. Most of them are. But there are a few that are not. The slurry jars on the other hand, are all 99% or finished.

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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22141323 - 08/25/15 03:20 PM (8 years, 10 months ago) |
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eat,
You ever slurried PE/APE grains for a tub yet?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22148657 - 08/26/15 08:06 PM (8 years, 9 months ago) |
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ive slurried pf jars with ape. not any grain yet. i may try it, i have a quart jar colonized of pe.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22148767 - 08/26/15 08:30 PM (8 years, 9 months ago) |
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I have a few colonized WBS of PE that I want to try it out on. I want to test a mini to get as quick of results as possible. Probably 1 slurried jar and 1 regular jar since it's only like a 20 gallon tote.
Just do everything the same except make the sub a bit less than FC due to the added water in the slurry?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22148907 - 08/26/15 08:52 PM (8 years, 9 months ago) |
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ive been doing my subs at field capacity lately without an issue. You would blend 2:1 grain to water slurry. then dump it in. dump i the 2nd quart. mix it all up and let it colonize.
i usually wait until full colonization before casing on the slurries.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22149288 - 08/26/15 10:17 PM (8 years, 9 months ago) |
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You spoon out half a quart of grain into a quart jar with a pint of water in it, blend, and then spoon in the other half and blend, right?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22149323 - 08/26/15 10:30 PM (8 years, 9 months ago) |
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yes. it will all fit after the first blend. you only need to blend for about 3 seconds the first time. then do about 10-15 after you add the 2nd half of the grain jar.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22149421 - 08/26/15 11:05 PM (8 years, 9 months ago) |
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Okay, sweet. I'll do that tomorrow night or something in a little mini. I'll keep you updated. Not 100% on the spawn unfortunately, but whatever.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22157099 - 08/28/15 03:05 PM (8 years, 9 months ago) |
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Okay, mixed in one quart jar regularly with a blended slurry jar to 4 quarts of sub. Like a dumbass, I forgot to put the blender attachment with the quart jar of water in the PC, so I wiped it down in a SAB with iso and made the switch. This tub will probably trich out or something, but whatever.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22157127 - 08/28/15 03:14 PM (8 years, 9 months ago) |
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For spawning i do it open air. You may be allright. A definite no no when inoculating grain though.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22157322 - 08/28/15 04:02 PM (8 years, 9 months ago) |
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Yeah, was planning on it like you did in the pics, but when I realized I had to use a dirty blade, figured I might as well take some precautions. I'll update you on the tub as it progresses.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22157410 - 08/28/15 04:24 PM (8 years, 9 months ago) |
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Quote:
eatyualive said: update below. so the slurry not only beat the LI but it also fully colonized the jars before most of the LI were complete. I dug in the back and pulled out a few jars of both the LI and Slurry jars up to the front. a few LI in the back weren't all the way done.
Quote:
eatyualive said:
SLURRY VS LIQUID INOCULANT
The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.
    
Here are some of the LI and Slurry jars that were in the back pulled to the front. As you can see, the LI jars are not 100%. Most of them are. But there are a few that are not. The slurry jars on the other hand, are all 99% or finished.

Slurry is very nutritive compared to LI. It makes sense is faster because the "jump effect" of the mycelium is greater when you go from high nutrient inoculant> to grains... than when you do from low nutrient inoculant> to grains. The jump effect is just slower with low nutrient inoculant.
Nice spawn eatu!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: Mr. Alien]
#22157933 - 08/28/15 06:48 PM (8 years, 9 months ago) |
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Thanks alien. Where have you been?
It has everything to do with the consistency of the mix actually. The nutrients may help but if i diluted the slurry to almost liquid like a glc it would have the same speed as an li or glc. When it is more mudlike the slurry will stick to or coat the grain. Whereas, with liquid inoculant the water may slide down the side of the grain. If you notice slurry that hits the side of the glass fuzzes up or even sticks to the glass. With li only agar chunks that stick to the jar may fuzz up. But the slurry fuzzes up everywhere.
Its all about viscosity.
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MushLover2552
Stranger

Registered: 08/13/15
Posts: 33
Last seen: 7 years, 11 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22157998 - 08/28/15 07:02 PM (8 years, 9 months ago) |
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Can't wait to try this... ordered the oster 14 speed for $20 and got an extra blade assembly locally to make the blender lid! I saw that you add slurry as extra spawn to tubs etc to give it a kick, ever tried adding GS to already-colonizing jars to speed them up?
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22158008 - 08/28/15 07:04 PM (8 years, 9 months ago) |
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Quote:
eatyualive said: Thanks alien. Where have you been?
It has everything to do with the consistency of the mix actually. The nutrients may help but if i diluted the slurry to almost liquid like a glc it would have the same speed as an li or glc. When it is more mudlike the slurry will stick to or coat the grain. Whereas, with liquid inoculant the water may slide down the side of the grain. If you notice slurry that hits the side of the glass fuzzes up or even sticks to the glass. With li only agar chunks that stick to the jar may fuzz up. But the slurry fuzzes up everywhere.
Its all about viscosity.
I agree. Do me a favor though, eat and make some masters with whole grain rice. Before G2Ging, add 75ml of sterile water and shake. It works great and you don't even have to blend anything. The rice doesn't have a hull, just a fragile thin film that holds its structure. That's why I cook mine slightly on the dry side. When you add the water and shake, it turns into a thick gravy like substance coating the rice that will coat your receiving jar grains just like your mud like slurry.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: insanemike]
#22158801 - 08/28/15 11:05 PM (8 years, 9 months ago) |
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are you talking about quart masters?
its almost like a GLC. ive done the same thing with wbs grain. where i simply swirled a spoonfull around in a quart jar and then poured that water into receiving jars. took about 9 days to colonize 30 quarts.
that sounds like shroom gravy. i do want to try it.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22158867 - 08/28/15 11:24 PM (8 years, 9 months ago) |
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You're just describing making LI by introducing colonized grains to sterile water, right?
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22159342 - 08/29/15 06:07 AM (8 years, 9 months ago) |
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Using whole grain rice is different than using grain with a thick hull like wbs or oats. Trust me, the whole grain rice makes all the difference. I think it's the starch in the rice that makes it thick like gravy. You don't pour the water out as an inoculant, the rice and "gravy" come out in globs, almost. It may even be more appropriate to spoon it out instead. It's the best inoculant for muda bottles by far and I'm almost positive it will be excellent for grain. I haven't gotten around to making anymore masters so I haven't been able to try it out on grain yet. So in the mean time, I thought you could give it a try because this type of thing is right up your alley, eat.
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DataVamp
Stranger

Registered: 08/12/15
Posts: 23
Last seen: 5 years, 11 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: insanemike]
#22174144 - 09/01/15 02:03 PM (8 years, 9 months ago) |
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Just read through this entire thread start to finish. Eatyu, this is an amazing system. Already gathering some of the supplies to give it a go.
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MushLover2552
Stranger

Registered: 08/13/15
Posts: 33
Last seen: 7 years, 11 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: DataVamp]
#22183965 - 09/03/15 05:30 PM (8 years, 9 months ago) |
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Blender came in!!!
 Has anyone slurried with the pitcher that comes with it? Personally I plan on using a plain ol' qt jar but it has a good pour, huge (1.25l or ~33% more than a qt) + a couple layers of tinfoil could seal it up in the PC..
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