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gator00
memeber over 10 yrs


Registered: 06/17/03
Posts: 262
Last seen: 7 years, 2 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22028402 - 08/01/15 01:17 PM (8 years, 10 months ago) |
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I have a question about the Tek. When adding the LI how do you correct the problem with extra water at the bottom of the jars. I mean even if you dry your grains very good dumping this into the jars would cause a water pool at the bottom wouldn't it?
-------------------- Anything I say in the above post is a complete lie. The voices in my head tell me to say it. And to burn things. any pictures that are posted are found on google.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: gator00]
#22028422 - 08/01/15 01:22 PM (8 years, 10 months ago) |
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you shake the jar for 20 seconds or up to a full minute in order to redistribute the moisture in the jar.
if your really worried, you could also add some dry verm to your grain jars. mix it in with the grain before you pressure cook.
Quote:
Machiavelliavore said: Hey, Just made some slurry last night in my pants with a a cup of colonized grass seed into half a cup of water. It came out extremely thick, almost like a paste. It was hard to pour and hard to shake . Just curious if grass seed might need more water for some reason, or is it just supposed to be super thick?
Do you know if an oster blender screwon base can go through the PC?
Also, any recommendations on lubricating an oster blender blade? It released a bunch of oil in the PC and it's fairly stiff now. Guess I shoulda siliconed it before use.
Apologies if any of this was answered in the thread, I wasn't quite ready to comb 30 pages.
so ive not tried it with grass seed. if its almost a mudlike and pasty consistency. then you will want to increase your water ratio to adjust for the grain. it should work, but if pouring is the issue, then definitely increase your water ratio. you may need to try a little at a time to figure out the perfect ratio for the rye grass seed. i definitely see it blending up into a mud like consistency like you said. not sure what ratio you used but if the 2:1 grain to water ratio is too low. add half as much grain or you can try adding more water for the same amount of grain.
no the black base can not go through the pc. it will melt. you can simply wipe it down with bleach wipes prior to bringing it into your SAB or clean work area. I use the base often if I am only using the blender blade assembly and switch lids after the blend. maybe wd-40 for the blade. you can also get a replacement package at walmart for 10 bux. or order from
http://www.goodmans.net/d/1377/oster-blender-blades.htm
   
see this thread for some great container builds to help inoculate.
http://www.shroomery.org/forums/showflat.php/Number/21927954/page/1
Edited by eatyualive (08/01/15 01:49 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive] 1
#22028604 - 08/01/15 01:46 PM (8 years, 10 months ago) |
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fairly soon im going to break this thread into pieces so its easier to find. i will also do a step by step for spawning directly to bulk subs using grain prepared by slury. and using colonize quarts to liquid spawn directly to bulk subs. ive been working on it for the last several months but have not had the time to finish things up.
also here are some updates on the pf classic slurry tubs.
   
  
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive] 1
#22034549 - 08/02/15 05:23 PM (8 years, 10 months ago) |
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Tub 1: This culture flushes evenly most of the time but these 5 tubs have some early bloomers. fatties as well.

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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22040042 - 08/03/15 10:15 PM (8 years, 10 months ago) |
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Tub 1 yield 7oz dry. took 3 harvests.
tub 4 isn't looking great. had to move some things around and loosen some poly. tubs 2-3 have dense fruits.
     late bloom

harvest with early bloomers

closeup tub 4

crazy mutant
  
decent harvest. dunked tub 1 for a 2nd flush. also below are the images of GLC/ GS2G jars from previous post prior to spawn. looking great. smelling great.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22040529 - 08/04/15 02:32 AM (8 years, 10 months ago) |
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 11 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: insanemike]
#22086463 - 08/13/15 11:51 AM (8 years, 10 months ago) |
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i love this tek, to me this makes life so much easier anywho heres my current expansion 9er tek clone to 1/2 pint pf-jar into 3/4 pint of sterile water blended dumped into 20 quarts of oats love it      eat i had the same mutant around christmas
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 11 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: tetherface]
#22088149 - 08/13/15 04:52 PM (8 years, 10 months ago) |
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have you done any testing with this tek on edibles? i'm about to slurry a quart of blue oyster oats out to more oats i'll post my results if anyones interested here's the B.O. quarts never grown them so I have no clue if their kosher or not but I think they are okay in comparison to pics ive seen on here 
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: tetherface]
#22088190 - 08/13/15 04:59 PM (8 years, 10 months ago) |
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Quote:
tetherface said: i love this tek, to me this makes life so much easier anywho heres my current expansion 9er tek clone to 1/2 pint pf-jar into 3/4 pint of sterile water blended dumped into 20 quarts of oats love it      eat i had the same mutant around christmas 
those are some psychedelic filters on ur lids there guy
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 11 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: blindingleaf]
#22088698 - 08/13/15 07:21 PM (8 years, 10 months ago) |
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I use those so my babies know what to be when they grow up lol
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: tetherface]
#22088725 - 08/13/15 07:27 PM (8 years, 10 months ago) |
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 i always whisper to them, but i will try that next time
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: tetherface]
#22090830 - 08/14/15 11:41 AM (8 years, 10 months ago) |
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Quote:
tetherface said: have you done any testing with this tek on edibles? i'm about to slurry a quart of blue oyster oats out to more oats i'll post my results if anyones interested here's the B.O. quarts never grown them so I have no clue if their kosher or not but I think they are okay in comparison to pics ive seen on here  
Someone tried it on straw with oysters. I would not use it on plain straw. It would be beneficial to use some kind of bulk additive such as dung when your adding extra moisture to a spawn bag or bulk sub. If your using slurry to expand grain. Then it should work fine. But if directly spawning to bulk you want to use subs that can absorb moisture. If one mixes finely chopped straw/dung/coir and verm it should be fine. I use pelleted wheat straw. But the pieces are pulverized into very small particle size. With the verm/coir addition to a sub it works perfecly.
Bo quarts look good. Here is a thread that you can compare to with some spawn shots.
http://www.shroomery.org/forums/showflat.php/Number/21744280/fpart/1/vc/1
Edited by eatyualive (08/14/15 12:51 PM)
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22090905 - 08/14/15 12:03 PM (8 years, 10 months ago) |
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Straw pellets FTW.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22092021 - 08/14/15 05:01 PM (8 years, 10 months ago) |
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Quote:
eatyualive said: Tub 1 yield 7oz dry. took 3 harvests.
tub 4 isn't looking great. had to move some things around and loosen some poly. tubs 2-3 have dense fruits.
     late bloom

harvest with early bloomers

closeup tub 4

crazy mutant
  
decent harvest. dunked tub 1 for a 2nd flush. also below are the images of GLC/ GS2G jars from previous post prior to spawn. looking great. smelling great.

Here are some 2nd flushes after a dunk. Diffused light was used on the 2nd flushes so the fruits are all over the place. dunked for a 3rd flush.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive] 1
#22113342 - 08/19/15 06:04 PM (8 years, 10 months ago) |
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SLURRY VS LIQUID INOCULANT
The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.
  
Edited by eatyualive (08/20/15 06:21 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22119280 - 08/20/15 10:41 PM (8 years, 10 months ago) |
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Damn, that's sick. When I have some clean LI, I'm going to knock up some BRF cakes so I can experiment with slurries again.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: stareatclouds]
#22120906 - 08/21/15 07:11 AM (8 years, 10 months ago) |
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I too want to get back into blending LI and pf slurry's again. But with my contam rate up with this summer weather, I think I might wait until average outside temperature begins to cool.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: insanemike]
#22121323 - 08/21/15 09:04 AM (8 years, 10 months ago) |
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Good idea with grain slurries. Pf jar slurries are fine. Although ive only had 2 tubs contam in the entire year of messing with the grain slurries. And i was using some high nitrogen based bulk additives. It happened right when the heat wave hit. I only use the bulk additives in the winter now for the same reason. . That extra 5 plus degrees in the summer is brutal whrn it hits. I have to keep the hvac temp lower by a few degrees to combat the warm weather.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22122482 - 08/21/15 01:29 PM (8 years, 10 months ago) |
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I have been keeping my ambient room temp between 66 and 70*F this summer. It's slowed down growth a bit but trich has been eliminated. However, now it's lipstck mold that is hurting my production. I'm not sure whether it was from the print, from when I transfered the invitro clone to another plate or when I transfered that to the LI jars. Whatever it was, I tossed all of my LI's from that batch. I do have 5 jars of that clone, that have clean growth. I will use these 5 jars for masters and use a kernel or 2 to put back on agar. I really need to learn the difference between cube myc and lipstick myc so I can transfer away when needed.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: insanemike]
#22122581 - 08/21/15 01:47 PM (8 years, 10 months ago) |
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Wow i run mine 76-82 and have very little contams. Beginning of summer always hits hard. Luckily this year has been very nice.
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