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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tourrat]
#21794869 - 06/11/15 08:36 PM (9 years, 20 days ago) |
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Quote:
tourrat said: Just nocced some muda style pp5 (hpoo/straw/verm/coir/brf/gyp) with a slurry from a cake (1.5" thick/ pint round) that had several large in vitro fruits (tiny heads thick stems,unopened veils) will report on how they recover.
Have done several batches of wbs as well as oats and have been super impressed with this tek...here's a pic of one of the in vitro jars to give an idea of fruit size.
did anything ever come of this?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: eatyualive]
#21964831 - 07/19/15 12:54 PM (8 years, 11 months ago) |
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Used GLC by taking one spoonful of colonized spawn, spooned it in a regular mouth quart jar with oster blade assembly. blended it for about 3-5 seconds. then poured that into 30 quart jars.
GLC jar build.

you can see on the left of the colonized quart(in the middle) are the Liquid inoculated jars on the right are the Grain Liquid Culture jars. I was going to do a side by side and see which one is faster. I think the LI will be as the GLC takes a little longer to establish. But, Only one spoonfull of mycelia was used illustrated in the op in carc's video.
the colonized quarts on the left side of this image are r44 p2g and burma masters. disregard those. you only want to look at the jars that are uncolonized.

updating op with carc's build.
Edited by eatyualive (07/19/15 02:33 PM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: eatyualive]
#21964877 - 07/19/15 01:03 PM (8 years, 11 months ago) |
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nice. i would guess the grain slurry would be faster than plate slurry, but u think its opposite?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: blindingleaf]
#21965343 - 07/19/15 02:22 PM (8 years, 11 months ago) |
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patiently waiting for update on that side-by-side
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: filthyknees]
#21965413 - 07/19/15 02:31 PM (8 years, 11 months ago) |
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sorry to clarify. disregard all colonized quart jars in the image above. the uncolonized quart jars are the jars that were inoculated. there is one colonized quart separating them on the right side. the colonized quarts on the left side are some p2g and masters of r44 and burma.
Quote:
blindingleaf said: nice. i would guess the grain slurry would be faster than plate slurry, but u think its opposite?
i was thinking the opposite. when i did the grain slurry prior it was about the same speed as the LI so i guess the side by side will tell. both are clones.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: eatyualive]
#21965441 - 07/19/15 02:36 PM (8 years, 11 months ago) |
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Hey, Eat. It's nice to see you active on the boards again.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: insanemike]
#21965475 - 07/19/15 02:40 PM (8 years, 11 months ago) |
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hey mike. journal land usa you know how it is.
had to get some things going quickly went back to some slurries. ive been messing with a bunch of different methods over the last 6 months. always find myself coming back to the slurries! least amount of work involved.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: eatyualive]
#21965675 - 07/19/15 03:05 PM (8 years, 11 months ago) |
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I haven't tried slurry since I was messing around with the muda bottles exclusively a few months back. I started to have trich problems as soon as the heat wave hit my area, so I haven't done an open jar inoculation since then. Everything is strictly flamed needle and ships.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: insanemike]
#21966011 - 07/19/15 04:05 PM (8 years, 11 months ago) |
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yeah i recently had two tubs trich out for the same thing. timing was horrible. had to change some things around in the grow room to get the temp down. even when the hvac is set to 76 its 80-82 in my gr. i like it to be 78 max. then there are never problems. finally got it down to that temp range after moving some things around.
but for all year the tub loss is very minimal. ill take the current ratio any day.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 11 months
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: eatyualive]
#22014013 - 07/29/15 09:37 AM (8 years, 10 months ago) |
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i made a thread about this but figured it was relevant here as well... leftover oat grain slurry discarded of in an aloe plant on my back porch and somehow it grew   its funny all my outdoor shrooms have turned out small like this but indoors they grow 8" tall all day long anyways my conclusion is that you can slurry to just about anything and have success, im gonna hit a different houseplant every time i use slurry to see if this was an isolated incident... thanks for the tek eats now i have endless grain spawn with very low contam rates but when i do get one its usually a 20 jar epic fail...lol
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 8 months
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: tetherface]
#22014060 - 07/29/15 09:49 AM (8 years, 10 months ago) |
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Niiice,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: tetherface]
#22014115 - 07/29/15 10:00 AM (8 years, 10 months ago) |
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Quote:
tetherface said: i made a thread about this but figured it was relevant here as well... leftover oat grain slurry discarded of in an aloe plant on my back porch and somehow it grew   its funny all my outdoor shrooms have turned out small like this but indoors they grow 8" tall all day long anyways my conclusion is that you can slurry to just about anything and have success, im gonna hit a different houseplant every time i use slurry to see if this was an isolated incident... thanks for the tek eats now i have endless grain spawn with very low contam rates but when i do get one its usually a 20 jar epic fail...lol
nice! I wonder if there are any nematode-consuming active species
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 11 months
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: Munchauzen]
#22014266 - 07/29/15 10:31 AM (8 years, 10 months ago) |
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I would imagine that there are a bunch of nematoads in that outdoor plant I wonder if we could test to see if they are consumed by introducing them to a sub at spawning then sampling a bit at full colonization and looking for their absence under a scope I bet that they're not consumed but live in symbiosis on a different note i think ive seen casing starter mix from a vendor claim to have nematodes in it to keep the fly larvae at bay so I may buy some and see if it gets overlayed if I wait an extra long time to fruit it maybe that we ould be an indicator as to which they are consumed or not ill check it under my foldscope
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: 3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant(UPDATE GRAIN SLURRY) [Re: tetherface]
#22014568 - 07/29/15 11:42 AM (8 years, 10 months ago) |
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Ha thats cool.
i used this build for some Penis Envy LI. uh oh....i got leap off the next day! genetics came from the Penis Master!


so my GLC and LI jars are almost finished. The LI jars won slightly. I had to shake both because they were taking forever(more than 4 days). Im spoiled by slurry. and the higher grain volume grain slurries. but for pure mycelial water, the colonization was just as fast as the LI from a grain jar. 9 days full colonization. even slow on my end for g2g. this last session I went real heavy on the LI with a pint of water to 1 plate. Poured 26 quarts of PE. had about 5 more jars worth of LI leftover. so this higher volume of water on the inoculation of LI actually showed leap off faster. I think the higher volume of water doesn't chop up the agar plate as much. There were much larger chunks than I normally have in the blender jar. I also pulsed it twice for about 3-5 seconds.
Grain slurry and slurry are take half the time as LI to colonize 100% without a shake. although ive compared them several times. i will still continue to do so in this thread. The LI and GLC took 9 days total with a shake.
day 1-9: only 20 quarts were used with LI. 30 used with the GLC. the jars on the right of the colonized quart are a Syzygy clone. Jars on the left are an amazon clone.
     day 7 after a shake on day 5
 
GLC Amazon
    
LI Syzygy
    
Edited by eatyualive (07/29/15 11:49 AM)
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bakenast
Muffen Stuffer


Registered: 03/14/11
Posts: 920
Last seen: 1 year, 5 months
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Re: 3 Day Quart Colonization: (Liquid Inoculant/PF Slurry/Grain Slurry/GLC) [Re: eatyualive]
#22019171 - 07/30/15 10:15 AM (8 years, 10 months ago) |
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just started reading your thread such a good idea, I recently got a osteriser gonna try this
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: bakenast] 1
#22025502 - 07/31/15 06:09 PM (8 years, 10 months ago) |
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ok this was a clone taken via 9er tek last year. so its well over a year old. the syringe of the stem tissue was stored in the fridge wrapped in a plastic bag. i still have about 12 syringes i have not even used because i am working off the clone from this one syringe for an entire year. so once injected, that pf jar was stored in the fridge for 6 months. i then slurried it and poured it into 50 quarts.

ive got a fun grow going now with a sandcastle that is this exact clone. no agar was used. and all slurried grain is being used. here are 4 tubs coming in nicely.
Edited by eatyualive (07/31/15 06:28 PM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#22025520 - 07/31/15 06:13 PM (8 years, 10 months ago) |
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beautiful spread man! paradise city
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#22025529 - 07/31/15 06:16 PM (8 years, 10 months ago) |
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Quote:
eatyualive said: ok this was a clone taken via 9er tek last year. so its well over a year old. the syringe of the stem tissue was stored in the fridge wrapped in a plastic bag. i still have about 12 syringes i have not even used because i am working off the clone from this one syringe for an entire year. so once injected, that pf jar was stored in the fridge for 6 months. i then slurried it and poured it into 50 quarts.
ive got a fun grow going now with a sandcastle that is this exact clone. no agar was used. and all slurried grain is being used. here are 4 tubs coming in nicely.


You da man, Eat.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: insanemike]
#22025747 - 07/31/15 07:03 PM (8 years, 10 months ago) |
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pin castle!
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 8 months, 16 days
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Re: Slurry Spawn or Liquid Inoculant [Re: filthyknees]
#22026135 - 07/31/15 08:51 PM (8 years, 10 months ago) |
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Hey, Just made some slurry last night in my pants with a a cup of colonized grass seed into half a cup of water. It came out extremely thick, almost like a paste. It was hard to pour and hard to shake . Just curious if grass seed might need more water for some reason, or is it just supposed to be super thick?
Do you know if an oster blender screwon base can go through the PC?
Also, any recommendations on lubricating an oster blender blade? It released a bunch of oil in the PC and it's fairly stiff now. Guess I shoulda siliconed it before use.
Apologies if any of this was answered in the thread, I wasn't quite ready to comb 30 pages.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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