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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 7 months, 21 days
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Re: Liquid spawn: Slurry Spawn (moved) [Re: tedoro]
#21604873 - 04/27/15 05:06 PM (9 years, 2 months ago) |
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Quote:
tedoro said: I was going to blend them in a clean but not sterile blender. Its my understanding that that is fine to do.
If you cannot heat it you can at least try and clean it as best you can. I would have it cleaned with washing up liquid and as hot water as you can manage. Then pour in some hydrogen peroxide. This should be shook about and I would leave it at least a day. I would then crack it open slightly in a SAB and drain off as much peroxide as I could.
If you wanted as much peroxide gone as possible it could be left in a warm place slightly cracked to let it evaporate off.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry Spawn (moved) [Re: blackout]
#21605171 - 04/27/15 06:02 PM (9 years, 2 months ago) |
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blackout! your one of my favorite posters.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,222
Last seen: 1 day, 3 hours
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Re: Liquid spawn: Slurry Spawn (moved) [Re: eatyualive]
#21607486 - 04/28/15 04:21 AM (9 years, 2 months ago) |
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cool, thanks blackout. I can't find anyone using rye berries for slurry. I'm only using it because its what I know. Perhaps it truly is more likely to contaminate...
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,222
Last seen: 1 day, 3 hours
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Re: Liquid spawn: Slurry Spawn (moved) [Re: tedoro]
#21607496 - 04/28/15 04:26 AM (9 years, 2 months ago) |
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Eats, Is there a place you are posting your most recent slurry to substrate trials? I'm very interested in a three quart slurry test I think I read you were doing....
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry Spawn (moved) [Re: tedoro]
#21607592 - 04/28/15 05:28 AM (9 years, 2 months ago) |
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Quote:
tedoro said: Eats, Is there a place you are posting your most recent slurry to substrate trials? I'm very interested in a three quart slurry test I think I read you were doing....
i have not done the 3 quart slurry test yet. i only did a 5 quart slurry test. thats next on the agenda but i have not had time to do so.
i did 1 quart of slurry, 4 quarts of regular spawn. its in the op.
i believe grainbrain had used oats before. i don't see why rye wouldn't work.
also i tested my tribal container and its leak proof. i had a minor setback as i accidentally ripped the silicone off of it when i was trying to widen the ship.
http://www.shroomery.org/forums/showflat.php/Number/21552063
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Liquid spawn: Slurry Spawn (moved) [Re: blackout]
#21608235 - 04/28/15 09:01 AM (9 years, 2 months ago) |
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Quote:
blackout said:
Quote:
tedoro said: I was going to blend them in a clean but not sterile blender. Its my understanding that that is fine to do.
If you cannot heat it you can at least try and clean it as best you can. I would have it cleaned with washing up liquid and as hot water as you can manage. Then pour in some hydrogen peroxide. This should be shook about and I would leave it at least a day. I would then crack it open slightly in a SAB and drain off as much peroxide as I could.
If you wanted as much peroxide gone as possible it could be left in a warm place slightly cracked to let it evaporate off.
i like it
i still think these were clean and I will prove it with the isolate im working on.
       
multiple flushes in poor conditions - yup. I will know for sure in a few months. This isolate will allow me to see the numbers to verify clean spawn. I'm excited!
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: Liquid spawn: Slurry Spawn (moved) [Re: blackdust]
#21609816 - 04/28/15 04:24 PM (9 years, 2 months ago) |
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I would not call that much of a flush.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,222
Last seen: 1 day, 3 hours
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Re: Liquid spawn: Slurry Spawn (moved) [Re: stareatclouds]
#21610646 - 04/28/15 07:40 PM (9 years, 2 months ago) |
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I'm excited! I'll slurry my 12 cup rye myco bag in 10 days and let you all know how it goes. I have three other non-slurry spawn tubs to compare.
T
PS.... when people say 5 quarts of spawn, don't they actually mean more like 15 cups? for the airspace left in a jar to mix? Thats been somewhat confusing. My 12 cups (the max I can go with four bags in my PC) feels like a tad bit less than most are using for a brick of coco and 2 quarts of vermiculite.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry Spawn (moved) [Re: tedoro]
#21610846 - 04/28/15 08:20 PM (9 years, 2 months ago) |
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Quote:
tedoro said: I'm excited! I'll slurry my 12 cup rye myco bag in 10 days and let you all know how it goes. I have three other non-slurry spawn tubs to compare.
T
PS.... when people say 5 quarts of spawn, don't they actually mean more like 15 cups? for the airspace left in a jar to mix? Thats been somewhat confusing. My 12 cups (the max I can go with four bags in my PC) feels like a tad bit less than most are using for a brick of coco and 2 quarts of vermiculite.
5 quarts 3/4 full. i sometimes over fill and the quarts end up being about 90% grain. but those are harder to shake if your g2ging. if your slurrying piece of cake.
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cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Liquid spawn: Slurry Spawn (moved) [Re: eatyualive]
#21612380 - 04/29/15 07:40 AM (9 years, 2 months ago) |
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095Y26Y???? whats that strain eat?
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry Spawn (moved) [Re: cArcace-x]
#21612503 - 04/29/15 08:36 AM (9 years, 2 months ago) |
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Just wanted to drop in and give you a little update of my experiences with pf slurries. From my initial observations with using a thick paste like slurry for the muda bottles, I have not been too impressed. The reishi bottles from the thick pf slurry are slower to colonize than the reishi bottles that I inoculated with a thin whole brown rice slurry. The reishi bottles with the WBR slurry were fully colonized in 3-4 days. The reishi bottles with thick pf slurry are 3-4 days in and are about a quarter of what the wbr slurried bottles were at that stage.
I used a thick pf slurry for most of my pe bottles and I am kind of dissapointed with progress so far. I did make up 8 more pe bottles yesterday with a very watered down pf cake and plan on making 8 more up today just like them. My paste like pf slurry was four ounces of water to one and a half 1/4 pint pf cakes. My watered down version is five ounces of water to one half 1/4 pint pf cake. If the watered down pf cake slurry has decent results, I may put it up against the thin WBR slurry.
Edited by insanemike (04/29/15 08:49 AM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry Spawn (moved) [Re: insanemike]
#21614035 - 04/29/15 04:52 PM (9 years, 2 months ago) |
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Quote:
insanemike said: Just wanted to drop in and give you a little update of my experiences with pf slurries. From my initial observations with using a thick paste like slurry for the muda bottles, I have not been too impressed. The reishi bottles from the thick pf slurry are slower to colonize than the reishi bottles that I inoculated with a thin whole brown rice slurry. The reishi bottles with the WBR slurry were fully colonized in 3-4 days. The reishi bottles with thick pf slurry are 3-4 days in and are about a quarter of what the wbr slurried bottles were at that stage.
I used a thick pf slurry for most of my pe bottles and I am kind of dissapointed with progress so far. I did make up 8 more pe bottles yesterday with a very watered down pf cake and plan on making 8 more up today just like them. My paste like pf slurry was four ounces of water to one and a half 1/4 pint pf cakes. My watered down version is five ounces of water to one half 1/4 pint pf cake. If the watered down pf cake slurry has decent results, I may put it up against the thin WBR slurry.
so for substrates its looking like more liquid is better? hmmm wild brown rice. nice. when i inoculated substrates with grain slurry the lower water volume works really well because the grain volume settles and its mostly water in the blend. it ends up looking like 90% water to 10% grain even though it isn't. i did 2:1 grain to water.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry Spawn (moved) [Re: cArcace-x]
#21614045 - 04/29/15 04:55 PM (9 years, 2 months ago) |
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Quote:
cArcace-x said: 095Y26Y???? whats that strain eat? 
syzygy.
and oldie but goodie revived from old prints from 2009. http://www.shroomery.org/forums/showflat.php/Number/11352880/fpart/1/vc/1
Edited by eatyualive (04/29/15 05:07 PM)
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cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Liquid spawn: Slurry Spawn (moved) [Re: eatyualive]
#21614108 - 04/29/15 05:06 PM (9 years, 2 months ago) |
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry Spawn (moved) [Re: eatyualive]
#21614158 - 04/29/15 05:16 PM (9 years, 2 months ago) |
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Quote:
eatyualive said:
Quote:
insanemike said: Just wanted to drop in and give you a little update of my experiences with pf slurries. From my initial observations with using a thick paste like slurry for the muda bottles, I have not been too impressed. The reishi bottles from the thick pf slurry are slower to colonize than the reishi bottles that I inoculated with a thin whole brown rice slurry. The reishi bottles with the WBR slurry were fully colonized in 3-4 days. The reishi bottles with thick pf slurry are 3-4 days in and are about a quarter of what the wbr slurried bottles were at that stage.
I used a thick pf slurry for most of my pe bottles and I am kind of dissapointed with progress so far. I did make up 8 more pe bottles yesterday with a very watered down pf cake and plan on making 8 more up today just like them. My paste like pf slurry was four ounces of water to one and a half 1/4 pint pf cakes. My watered down version is five ounces of water to one half 1/4 pint pf cake. If the watered down pf cake slurry has decent results, I may put it up against the thin WBR slurry.
so for substrates its looking like more liquid is better? hmmm wild brown rice. nice. when i inoculated substrates with grain slurry the lower water volume works really well because the grain volume settles and its mostly water in the blend. it ends up looking like 90% water to 10% grain even though it isn't. i did 2:1 grain to water.
I would say for muda bottle substrates, more liquid is better because it seems to mix and be more evenly distributed throughout. If spawning a tub or tray, the thicker slurry may work better since you can sort of massage it into the substrate. But when you have to shake the thick slurry into the muda bottles, it seems to have a snow ball effect. As in it forms a ball of substrate around it as it begins to roll around. After that I seem to only be able to fracture the ball and break it down into a handful of smaller balls of slurry which create too few inoculation points.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry Spawn (moved) [Re: insanemike]
#21614179 - 04/29/15 05:20 PM (9 years, 2 months ago) |
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ah snowball effect sounds logical.
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camplo
Freedom!


Registered: 02/10/08
Posts: 1,675
Last seen: 2 years, 9 months
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Re: Liquid spawn: Slurry Spawn (moved) [Re: stareatclouds]
#21625329 - 05/02/15 09:37 AM (9 years, 1 month ago) |
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Quote:
stareatclouds said: I would not call that much of a flush.
I think hes rolling with the same concept that I am with taking a clone from the pins that grew off of my LC. If the genetics allow for fruiting in the poorest conditions then in the optimal conditions maybe it will fruit prolifically. Atleast thats what i'm going for. I have a jar that I believe to have bacteria, the jar colonized slower than shit. I want to get a clone from it as well.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry Spawn (moved) [Re: camplo]
#21625438 - 05/02/15 10:17 AM (9 years, 1 month ago) |
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Camplo, I have always been curious of that too. Genetically gifted mushrooms should, theoretically, give you better results in the poorer conditions than that of mushrooms with weaker genetics.
Edited by insanemike (05/02/15 10:22 AM)
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 13 days
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Re: Liquid spawn: Slurry Spawn (moved) [Re: insanemike]
#21638151 - 05/05/15 04:34 AM (9 years, 1 month ago) |
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I have 2 fully colonised bags. I was thinking of Taking a few handfulls and slurrying some of the grains. What do you reccon?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: Liquid spawn: Slurry Spawn (moved) [Re: camplo]
#21639425 - 05/05/15 01:11 PM (9 years, 1 month ago) |
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Quote:
camplo said: I think hes rolling with the same concept that I am with taking a clone from the pins that grew off of my LC. If the genetics allow for fruiting in the poorest conditions then in the optimal conditions maybe it will fruit prolifically. Atleast thats what i'm going for. I have a jar that I believe to have bacteria, the jar colonized slower than shit. I want to get a clone from it as well.
But you'd find out if the genetics are awesome by fruiting in optimal conditions too. He just wants to prove you don't have to be sterile to get fruits. And then he'll always post a shitty grow as results. Which is weird because when he does follow optimal procedure, he gets beautiful grows.
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