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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Edmunter]
#21558446 - 04/17/15 05:32 AM (9 years, 2 months ago) |
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Quote:
Edmunter said: I have half a jar left over that is begining to myc up again. What can I do with this?
pf jar? or quart grain? i usually dump jars that i have already slurried. im a little sketchy about using a jar over and over after ive opened it once. often if its grain leftover. i may g2g the rest of the leftover into a few quarts.
you could attempt it, but know that it may be a risk.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: hamloaf]
#21558450 - 04/17/15 05:33 AM (9 years, 2 months ago) |
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it was beginning to look funky in the last round of pics. sux, you have a plethora of colored funk going on in there. what volume did you pour into each bag?
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21558632 - 04/17/15 07:29 AM (9 years, 2 months ago) |
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About 4-5 ml's per bag. The bags did have filter patch, btw. 
Pretty sure the cake was dirty due to the contam showing up before the two week mark from inoculation, as well as, the pattern in which the contamination showed up as.
Another symptom of the cake being contaminated was that there was slight pinning happening at about the 3 week mark after full colonization of the cake in the jar was reached before the cake was slurried. Didn't pay much mind to the pinning, though, because the cakes had had over 2 weeks of consolidation.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: hamloaf]
#21564686 - 04/18/15 05:29 PM (9 years, 2 months ago) |
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i generally use the pf cakes at full colonization or about 2 days after full colonization. if i don't use them, i stick them in the fridge until im ready to use them.
did you see what tourrat did with fat invitro pins and all?
http://www.shroomery.org/forums/showflat.php/Number/21527601#21527601
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21567419 - 04/19/15 10:31 AM (9 years, 2 months ago) |
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here is a pic of using slurry with oven pasteurized grain supplementation (test 2). About 60% grain supplementation. Work was done in open air. Used two qts of spawn for the slurry. Lost tub to some trich.
*Note: this is done in un-sterile procedures. As we can see in the picture I got close. Showing the potential that slurry can have in other ways.

I feel pretty good that test 3 will come out 100% clean. I just have to change a few things.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: blackdust]
#21568227 - 04/19/15 02:15 PM (9 years, 2 months ago) |
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how did you prep the grain for supplementation? what is the substrate?
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21568272 - 04/19/15 02:26 PM (9 years, 2 months ago) |
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So eat. I've skimmed through this thread, but let me see if I have the concept down. If I made a wide mouth blender attachment, and used it to slurry a half gallon of clean spawn, I could use one half gallon per 66qt tub instead of 3 by virtue of more inoculation points? So I just pour some sterile water into the grain jar inside the SAB then blend up?
I think I need a few more years of experience before I try this, but that is so fucking cool!
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21568642 - 04/19/15 03:47 PM (9 years, 2 months ago) |
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Quote:
eatyualive said: how did you prep the grain for supplementation? what is the substrate?
Has about half a gallon, ziplock bag worth of WBS(measured dry). About 5 cups of BRF. And of course coir. So highly supplemented. i baked the sub in the oven for a few hours.
For test 3, I will bake the sub in what would be later be the FC. I will also inoculate in a SAB. I figure I could do trays with just grain flour, some with just whole grains, and some with a mix of both. I think the sterile work will give this success every time. I should have a clone in a few weeks for isolating. Then I can use the isolate for more tests. I want to see if I can prove clean spawn.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: NumeroEno]
#21570632 - 04/20/15 05:54 AM (9 years, 2 months ago) |
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Quote:
NumeroEno said: So eat. I've skimmed through this thread, but let me see if I have the concept down. If I made a wide mouth blender attachment, and used it to slurry a half gallon of clean spawn, I could use one half gallon per 66qt tub instead of 3 by virtue of more inoculation points? So I just pour some sterile water into the grain jar inside the SAB then blend up?
I think I need a few more years of experience before I try this, but that is so fucking cool!
Get a bunch of good bulk grows in. Once you have mastered grain and g2g transfer. This would be the next step to try. Its a little different feel to get liquids working properly. Some can get it right away that have the right touch. Others may take a few trial and error sessions to get the technique and mastery down. But for me one of the key points is not to overdo anything when you add liquids. Ill even at times adjust grain prep and moisture content of media to accommodate the added liquid. Most give up after one or two failures. Hell I had a few failures with liquid cultures myself trying to learn how to do it. But once learned, it is an amazing art form. Liquids are like painting on canvas to me.
Kind of. I do two different things depending on what size jars i have available to use.
since your talking about using a half gallon. if you wanted to blend that directly to bulk. use 8-9 quarts of substrate volume. you could pressure cook another half gallon jar with a lid attachment and 1.5 quarts of water in it. For the bulk slurry I used 3/4 quart water to 1 quart grain. You have two options on transfer: 1) shake the grain jar until it is broken up completely. Then in your SAB dump that into the jar with water and blade assembly. 2) Break up your grain jar. Pour the water from the blender jar into the grain jar. Switch lids of the blade jar to your now grain and water jar.
You then blend this with your power drill adapter attachment and pour in open air as you would normally spawn. I've tried it both ways and they both work. Do I prefer one or the other? I am not sure yet. I have been alternating between the two and it seems to be very quick to dump the broken up grain into the water/blade jar. If you do it the other way it is a little more difficult switching the lids but not much.(im currently updating all of this info with a more detailed procedure in the works)
Additionally, I think for decent yield you will want to use more than 2 quarts. I would go 3 at the least. What I have been doing lately that has been working extremely well, is use a 1 Quart jar of slurry and spawn the rest of my spawn normally. So lets say I spawn with 5 total quarts of grain. I will blend up 1 Quart jar slurry for spawn. Then spawn the other 4 quarts of grain normally. This speeds up my spawn run by 1-2 days on average compared to normal spawning speeds in my setup with my environmental controls and temps around 76-83 degrees.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive] 1
#21575644 - 04/21/15 08:58 AM (9 years, 2 months ago) |
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This is what I have so far. I used a 1/4 pint to noc up 8 muda bottles. I went with your 2:1 water to cake ratio, eat. Since no one has an answer yet for how much slurry to use or how thick the slurry should be for the muda bottles I opted to run a little experiment. Instead of shaking the slurry in between pours, the first bottle from each slurry jar naturally has more liquid inoculant and the 4th and final bottle from each slurry jar has more solid inoculant. If anyone is interested, I will keep updating progress.
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KindofBlue
The truth is out there


Registered: 10/14/14
Posts: 151
Last seen: 3 years, 11 months
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: insanemike]
#21575906 - 04/21/15 10:52 AM (9 years, 2 months ago) |
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Threads like this are what make this place awesome. Thank you.
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 2 months
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: KindofBlue]
#21575918 - 04/21/15 10:58 AM (9 years, 2 months ago) |
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Quote:
KindofBlue said: Threads like this are what make this place awesome. Thank you.
QFT
I'm over here just taking notes like
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Credit where credit is due.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: insanemike]
#21576370 - 04/21/15 01:56 PM (9 years, 2 months ago) |
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Quote:
insanemike said: This is what I have so far. I used a 1/4 pint to noc up 8 muda bottles. I went with your 2:1 water to cake ratio, eat. Since no one has an answer yet for how much slurry to use or how thick the slurry should be for the muda bottles I opted to run a little experiment. Instead of shaking the slurry in between pours, the first bottle from each slurry jar naturally has more liquid inoculant and the 4th and final bottle from each slurry jar has more solid inoculant. If anyone is interested, I will keep updating progress.

also mike you can simply use the black base attachment on the top of those jars and it will blend up perfectly. the gasket when pouring is what you have to look out for. or you can build a badass rig like carc did below.
nice mike. yeah if your the first trying it, it may take some ratio experiments for the inoculant. i think if you stick with what you generally use for a bottle its probably similar. if im doing 2 quarts slurry spawn to 9 quarts of substrate that may be able to give you a rough idea of how much inoculant im using in a larger tub. maybe you can figure out the ratio in what volume substrate your using.i generally use about a tablesppon volume per grain jar. im thinking it might actually need a little more when substrate is involved as the substrate may absorb the liquid a little easier.
yeah im doing something similar with grain slurries. where i use the liquid inoculant water first. then pour the blended up grain on the last 10 or so jars. the jars with grain chunks grow about 2-3 days faster than the GLC jars. i posted some links a few pages back.
http://www.shroomery.org/forums/showflat.php/Number/21554067#21554067
http://www.shroomery.org/forums/showflat.php/Number/21242497#21242497
also i built this awesome container that carc made! easy build, good write up! im going to use it on my next slurry.
http://www.shroomery.org/forums/showflat.php/Number/21552063
Edited by eatyualive (04/21/15 02:03 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: KindofBlue]
#21576373 - 04/21/15 01:57 PM (9 years, 2 months ago) |
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Quote:
KindofBlue said: Threads like this are what make this place awesome. Thank you.
thanks for the kind words.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21576482 - 04/21/15 02:34 PM (9 years, 2 months ago) |
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Thanks for the links. I skimmed over carc's rig tek a few days ago, the shit is boss. I just wanted to take this one step at a time. I'm very new to pf, slurries and muda bottles and I'm trying to make the all work together. When I'm comfortable with what I'm doing I will damn sure add carc's rig to the toolbox.
I used about 30ml for each bottle. I did notice a clump in the substrate from the thicker part of the inoculant. I think I broke it up well enough because I couldn't find it again after a real hard shake. If you want, I'll post pics/ updates of progress on your thread, if you like?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: insanemike] 1
#21578024 - 04/21/15 08:10 PM (9 years, 2 months ago) |
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yes the muddy part is the part that clings to grain really well. good plan. i always had good success using plain blender jar/gasket/black base and blender. pouring from the side of the jar nothing fancy.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,222
Last seen: 1 day, 3 hours
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Re: Liquid spawn: Slurry Spawn (moved) [Re: eatyualive]
#21601193 - 04/26/15 08:24 PM (9 years, 2 months ago) |
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Just got done reading this thread. I am very interested in doing a slurry direct to substrate. I am so excited about what you all have accomplished.
My objectives are to do the most harvest with the least spawn, and to reduce the spawn to fruit times.
Will this work?
1) One 3 quart, fully colonized myco bag of rye berries slurried with two quarts of water. (golden teacher)
2) substrate made like frank (1brick of coco/ 2 quarts of verm / handful gypsum) and instead of 4.5 quarts of water, I would try to do it with 2.5 quarts of water.
I am currently getting 4 ounces dry from 3 quarts of rye spawn in 4 weeks (spawn mixed into substrate to last fruits). about three flushes.
I am concerned about rye berries contaminating in the slurry. And if I can pasteurize the coco with that little water.
Thoughts?
Edited by tedoro (04/26/15 08:29 PM)
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Liquid spawn: Slurry Spawn (moved) [Re: tedoro]
#21601325 - 04/26/15 08:53 PM (9 years, 2 months ago) |
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How do you plan on slurrying the grain if it's in a bag? If you're going to dump them into a jar to slurry, you might as well grow the spawn in jars in the first place.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Liquid spawn: Slurry Spawn (moved) [Re: insanemike]
#21602933 - 04/27/15 08:29 AM (9 years, 2 months ago) |
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Nothing spectacular, but this is a minimono from one of the pin covered cakes.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,222
Last seen: 1 day, 3 hours
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Re: Liquid spawn: Slurry Spawn (moved) [Re: grainbrain]
#21603540 - 04/27/15 11:16 AM (9 years, 2 months ago) |
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I was going to blend them in a clean but not sterile blender. Its my understanding that that is fine to do.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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