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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: fishy1]
#21442824 - 03/22/15 11:53 AM (9 years, 3 months ago) |
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not much sleep on top of real life and work. it does help that i love cultivation.
Edited by eatyualive (03/22/15 11:54 AM)
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thisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 8 years, 17 days
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21452847 - 03/24/15 07:57 PM (9 years, 3 months ago) |
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So I'm going to do a G2G slurry for the first time. The only thing is the half pint of colonized rye I have is in a wide mouth jar where the blender assembly fits a regular mouth jar. Could I possibly just break up the colonized jar, then add it to the jar with the PC'd water in it?
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: thisisfielding]
#21456793 - 03/25/15 06:10 PM (9 years, 3 months ago) |
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Just keep everything sterile & you should be fine. For the PFSlurry, you're adding a cake to the water jar.
Speaking of which, I have a fully colonized & consolidated cake that is pinning in the jar. Some of the pins are 2" long. This one is getting pureed in the morning at a 1:1 ratio for extra thick slurry goodness, then out to a dozen jars.
The four monotubs from the last slurry are all in fruiting conditions curently.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: grainbrain]
#21456891 - 03/25/15 06:30 PM (9 years, 3 months ago) |
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Quote:
grainbrain said: Just keep everything sterile & you should be fine. For the PFSlurry, you're adding a cake to the water jar.
Speaking of which, I have a fully colonized & consolidated cake that is pinning in the jar. Some of the pins are 2" long. This one is getting pureed in the morning at a 1:1 ratio for extra thick slurry goodness, then out to a dozen jars.
The four monotubs from the last slurry are all in fruiting conditions curently.
do you have any pictures of those tubs that you slurried with the pins on it? id like to see some pics.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21457204 - 03/25/15 07:37 PM (9 years, 3 months ago) |
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The pinning cake gets slurried tomorrow, then spooned to 12 jars. I'll post pics once it starts popping.
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thisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 8 years, 17 days
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: grainbrain]
#21458512 - 03/26/15 03:46 AM (9 years, 3 months ago) |
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quick question : if i shook a jar that was 100% colonized a couple days ago and its almost fully recovered can i use that for the slurry still or should i wait until its fully recovered?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21461990 - 03/26/15 06:17 PM (9 years, 3 months ago) |
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Quote:
eatyualive said: do you have any pictures of those tubs that you slurried with the pins on it? id like to see some pics.
   
   
Been trying to figure out why this tub sucked so bad for so long and I just realized it was my WBS + WBS Slurry tub that I slurried with a cake that had a few pins on it, I think. Maybe that's why it's such a shitty performer? Maybe just bad genetics.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: thisisfielding]
#21462284 - 03/26/15 07:22 PM (9 years, 3 months ago) |
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Quote:
thisisfielding said: quick question : if i shook a jar that was 100% colonized a couple days ago and its almost fully recovered can i use that for the slurry still or should i wait until its fully recovered?
id wait till its 100% before slurrying it again. you may even give it a day or two after its 100%.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: stareatclouds]
#21462294 - 03/26/15 07:24 PM (9 years, 3 months ago) |
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was it multispore? mine been rocking with clones. all of them.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21462360 - 03/26/15 07:46 PM (9 years, 3 months ago) |
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The BRF jar was noc'd with an MS LI that I found in my fridge. I'm bad at keeping records of stuff I use, but I'll have to change that soon.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: stareatclouds]
#21462386 - 03/26/15 07:54 PM (9 years, 3 months ago) |
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i think you had a delay in inducing fruiting in this tub didn't you mention that? how long did you wait?
Edited by eatyualive (03/26/15 07:54 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21462523 - 03/26/15 08:28 PM (9 years, 3 months ago) |
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Yeah, good memory. My note says I was 3-5 days late on fruiting, but that's like 3-5 days past a full 100% coverage. Think that fucked up the sub?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: stareatclouds]
#21463466 - 03/26/15 11:05 PM (9 years, 3 months ago) |
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no. that shouldn't have. i thought you meant more like 2 weeks or something.
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Mr. Penguin


Registered: 03/26/14
Posts: 548
Last seen: 6 years, 2 months
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
#21463695 - 03/26/15 11:52 PM (9 years, 3 months ago) |
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Got 2 66qt mono tubs consolidating as we speak. should be done at the end of this weekend.
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camplo
Freedom!


Registered: 02/10/08
Posts: 1,675
Last seen: 2 years, 9 months
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Mr. Penguin]
#21464538 - 03/27/15 08:23 AM (9 years, 3 months ago) |
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This slurry shit is good business. The fact people aren't getting mass contamination is awesome, I came across some threads from you, eatyu, and in the beginning even you were skeptical. The miracle is that once you slurry the grains you are exposing uncolonized grain, a mass amount at that, which is normally a no no.
I want to experiment with slurry, just waiting on my grain jars to finish. I created the "camplo gallon slurry jars" (laf) just for said purpose but it turns on the blender blade assembly is not a 100% seal and for long term storage, isn't safe. Still the idea might work. I want to take freshly inoculated grain, slurry it, and then see how it colonizes. The idea would be to get the growth speed of a LC culture but the nutrient power of grain.
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Edited by camplo (03/27/15 08:24 AM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: camplo]
#21469588 - 03/28/15 09:12 AM (9 years, 3 months ago) |
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camplo do you have a link? ill follow the thread. im interested to see your experiments. also feel free to post your trials in the tribal forum if you wish. click my sig below that says "the tribe" and it will link you to the forum.
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thisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 8 years, 17 days
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So I'm getting my Series 1 Flowhood in the mail on Tuesday and I'm going to proceed doing my first slurry work. I just want to clarify a couple things to make sure I'm reading things correctly.
I can either - 1. Take PF Cake, slurry it and use it to inoculate grain jars. 2. Take PF Cake, slurry it and then pour it into pasteurized substrate (in my case its going to be the 50/50 coir / vermiculite / gypsum) which goes into monotub. 3. Take PF Cake, slurry it and take uncolonized grain jars and pour all of this into mono tub with my pasteurized substrate.
Also pretty much do the same thing with a colonized grain jar as well instead of PF Cake.
Does this sound correct? My brain always glazes over with the spawn/substrate convos cause it always gets me mixed up.
Is there a benefit by taking 1 slurry jar to inoculate 5 uncolonized grain jars (waiting 3 days) then putting it into a monotub with pasteurized 50/50 cvg VS taking 1 slurry jar and 5 uncolonized grain jars and pouring it into the monotub (using the slurry to colonize the grains + substrate in the actual monotub)?
I have both colonized PF jars and grain jars waiting to do this slurry, I want to wait until I get my flowhood setup before I try this for the first time and trying to figure out what my best route to go.
Currently I have multiple empty monotubs ready, 10+ Quart grain jars colonized, 10+ half pint PF cakes colonized, 20 jars of uncolonized grain PC'd and as much substrate as I need (just need to pasteurize it when need be). What would be your best advice on what I should do to produce the best yield the quickest - I've been waiting for a while to get to the fruiting phase and I'm so close and very excited.
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Nos-hroom
Oneironaut's Apprentice



Registered: 08/13/14
Posts: 183
Last seen: 9 years, 7 days
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I don't believe #3 is quite right, unless I've been doing a terrible job at following along.
Eat mixed slurried colonized grain with grain spawn to a mono, which had rather excellent results.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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#3 is wrong. You'd combine the slurry with colonized, clean spawn. In fact, you'd likely want to combine #1 and #3. Use BRF cakes to make slurry and to noc up a bunch of spawn and then you can either slurry that spawn itself (grain slurry) or mix that spawn in with another slurried cake.
I'm not sure if the process has changed, but I couldn't get tubs to fully colonize with even 2 BRF cakes in there. But with a slurry AND the colonized grain, I got fruits.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Quote:
thisisfielding said: So I'm getting my Series 1 Flowhood in the mail on Tuesday and I'm going to proceed doing my first slurry work. I just want to clarify a couple things to make sure I'm reading things correctly.
I can either - 1. Take PF Cake, slurry it and use it to inoculate grain jars. 2. Take PF Cake, slurry it and then pour it into pasteurized substrate (in my case its going to be the 50/50 coir / vermiculite / gypsum) which goes into monotub. 3. Take PF Cake, slurry it and take uncolonized grain jars and pour all of this into mono tub with my pasteurized substrate.
Also pretty much do the same thing with a colonized grain jar as well instead of PF Cake.
Does this sound correct? My brain always glazes over with the spawn/substrate convos cause it always gets me mixed up.
Is there a benefit by taking 1 slurry jar to inoculate 5 uncolonized grain jars (waiting 3 days) then putting it into a monotub with pasteurized 50/50 cvg VS taking 1 slurry jar and 5 uncolonized grain jars and pouring it into the monotub (using the slurry to colonize the grains + substrate in the actual monotub)?
I have both colonized PF jars and grain jars waiting to do this slurry, I want to wait until I get my flowhood setup before I try this for the first time and trying to figure out what my best route to go.
Currently I have multiple empty monotubs ready, 10+ Quart grain jars colonized, 10+ half pint PF cakes colonized, 20 jars of uncolonized grain PC'd and as much substrate as I need (just need to pasteurize it when need be). What would be your best advice on what I should do to produce the best yield the quickest - I've been waiting for a while to get to the fruiting phase and I'm so close and very excited.
yes but the pf cake directly to the substrate is a very low spawn ratio. results are inconsistent and unless you have a good clone, may not perform well. the grain slurries directly to bulk work very well. i generally use the slurry itself to expand large volumes of spawn that i then may spawn normally into tubs or slurry those grain jars directly to substrates.
like stare said, 3 is wrong. you always want to sterilize everything and use the clean work technique like your flowhood. i use a similar technique to doing grain to grain transfers and that works well.
well the idea is that you expand that one pf jar into 50-100 quarts of grain. if your going to use it for 5 quarts. you would not need to use but 1/4 of the pf cake. so i would just use a sterile fork to fork the pf jar into grain jars like a grain master only your using the pf jar. you can look at the link below for p2g transfers. that is what i do when i only do about 10 jars per pf jar. it depends. if i only have 10 quarts available i might only fork the pf jar into the grain jars. it takes around 6 days to get full colonization.
P2G Transfer
why this is so great is. if you work lets say 60 hours a week. you can prep 10 quart jars a night. by the end of the week, you have 50. or if you have a large enough pressure cooker you may be able to make 100. you take that 1 pf jar on saturday morning, slurry it and in 3-4 days you have 50-100 quart jars of grain colonized and ready for spawning. so, on a busy schedule, its boss. you no longer have to wait for agar to colonize for 2 weeks. or even a stubborn LI to colonize in a week and a half. GLC takes about as long as an LI to colonize as well. so its very fast. the key difference between LI, GLC and slurry is that slurry has chunks of grain or brf mix that allow the mix to be a very mud like consistency. this mudlike consistency sticks to the grain instead of sliding past it like some LI's do. this also has setbacks as far as using it in lets say syringes or smaller width tubing to use as injection. it would clog easily. but pouring is very easy directly from your blend jar.
and for an example, i have stored my pf jars in the fridge for months and up to a year.i just take one out when im ready to slurry. some cube varieties last longer in the fridge than others. tas, burma, tex, thai all last a long time up to a year. i make pf jars about every 6-8 months. then i store the pf jars. and that will last well over a year from one pf jar to make enough tubs without having to start from master again for a full year or more. ill continue after that slurry transfer. take one of those colonized quarts after i have spawned the other 49 and slurry it to a 2nd round of 50-100 quarts. so it exponentially is limitless. im doing far less work than most realize. even though it may appear to look complicated but i assure you, im sitting back and relaxing. while others are constantly doing busy work. i did busy work for a very long time in this hobby. but ive figured out ways around that. i only need to do agar work once a year to have enough spawn/master material for more than a years worth of grows. its boss.
i just slurried an ape multispore pf jar that was made in march 2013. and i just spawned 6 tubs from that one pf jar. i also have 8 more quarts ready for spawning. that ape multispore was from a syringe that i had stored in the fridge from 2009. i inoculated that syringe around february of 2013. the spawn jars turned out great, smell great and i spawned all of them normally to bulk subs.
Edited by eatyualive (03/30/15 05:44 AM)
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