|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Slurry Spawn or Liquid Inoculant [Re: grainbrain]
#21388370 - 03/10/15 05:43 PM (9 years, 3 months ago) |
|
|
i don't necessarily consolidate the jars before using them. i wait till full colonization and give them a day or two after then use them. or i store them in the fridge immediately after that.
so here is my 5 quart tubs. 4 quart regular spawn, 1 quart slurry 4th flush harvest of 50.5g.

in addition, i got my first slurry contam in my 8 quart spawn tubs before 1st flush. some trich got a hold of one spot and i isolated it with some salt. but the tub flushed well. this is the first contam in close to 40 tubs. so im not at all dissatisfied with the results. the temps jumped up with spring and the growroom got above 85. but the other 3 tubs of this batch fruited well over 8z each. i had one tub fruit 12z dry. total yield on 3 tubs was 24z dry with 8 quarts of spawn in each tub. with about a 1/3" casing layer on top.
 
also some fun gifs of some earlier slurry tubs!!

|
wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21388398 - 03/10/15 05:50 PM (9 years, 3 months ago) |
|
|
Heelllll yeah
|
hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 1 hour, 36 minutes
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21388413 - 03/10/15 05:54 PM (9 years, 3 months ago) |
|
|
Noice! Those gifs are some next-level shit (no pun intended). Thanks for sharing, sir. One!
|
magicMerlin



Registered: 01/26/12
Posts: 617
Loc: Toronto
|
Re: Slurry Spawn or Liquid Inoculant [Re: hamloaf]
#21393477 - 03/11/15 03:31 PM (9 years, 3 months ago) |
|
|
Eat, I had made a PF slurry with a jar that had 120+ pins forming invitro. I have 3 tubs fruiting right now and they are the most insane thing I've grown. I did a top layer with leftover coir & verm on one and the pinset is out of this world. The others without top layers are also performing better than any MS or limited MS grow I've done. I wish I was more confident in posting pictures.
Question: Lets say I've inoculate a PF cake with MS and let it fully colonize, maybe even let it pin invitro as I did. If I use this colonized cake as inoculant as per your tek, are the genetics going to be more limited than MS? If so, do you any idea as how limited? I've never had a pinset as even as these with MS.
Thanks for all the tips Eat
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Slurry Spawn or Liquid Inoculant [Re: magicMerlin]
#21394134 - 03/11/15 05:35 PM (9 years, 3 months ago) |
|
|
hey can you post pics?
the substrate as casing method really makes for insane pinsets. but i can get pretty decent results with casings and multispore consistently with many varieties. i think maybe you just found the right recipe for success. click my signature that says the tribe. if you have time, make a grow log.
the fact that your having success even with fruitbodies tells me one thing, with proper procedure anything can be possible when it comes to innovation. maybe because the cake was already in fruiting mode it helped. but i have no idea. it could be a detail to remember for the future when you try it a few more times.
remind me, was the slurry cake you used poured into grain jars then fruited? or was it spawned to substrate directly?
Edited by eatyualive (03/11/15 05:41 PM)
|
tourrat
humanbeinganimal


Registered: 01/16/14
Posts: 394
Last seen: 8 years, 9 months
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21394406 - 03/11/15 06:26 PM (9 years, 3 months ago) |
|
|
I've got 24 jars I'm about to slurry nocc tomorrow. Will post updates. Thank you all for the info....really great stuff here....
About the pins in vitro...I think I remember reading something Stamets said about hormones in pins making for good spawn. That was in relation to grain jars having a pin or two. I could certainly see where a lot of pins could result in a massive hormone dose leading to a crazy pin set. I wish I remembered more about whatever it was I read.
-------------------- Knee deep in the hotel tub.....
|
magicMerlin



Registered: 01/26/12
Posts: 617
Loc: Toronto
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21396058 - 03/12/15 04:26 AM (9 years, 3 months ago) |
|
|
Quote:
eatyualive said: remind me, was the slurry cake you used poured into grain jars then fruited? or was it spawned to substrate directly?
I was in a hurry but to excited not to share earlier; I should have clarified some things. First, my PF cake was inoculated with LI made with limited, but tested, genetics. The cake was fully colonized and I set it aside to consolidate for 7 days. About 3-4 days later I checked and the thing had over a hundred healthy pins popping up so I cut consolidation short and made a slurry. I slurried 1/2 the cake (pins and all) to about 10 jars of WBS. I saw recovery within a few days (faster than my LI personally). There are TONS of beautiful even cluster on all tubs, but the best looking one has the C/V top layer.
My previous question clarified: Is inoculating with a PF cake akin to inoculating with a clone in terms of genetics? Example, if using MS, am I getting tons and tons of different genetics when using the cake as inoculant, or has the colonization limited them? This might be a dumb question I just want to make sure.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Slurry Spawn or Liquid Inoculant [Re: magicMerlin]
#21396190 - 03/12/15 05:51 AM (9 years, 3 months ago) |
|
|
i don't see that the genetic variation on multispore would be any different if you were using any other methods for growing cubes. it is still multispore.
|
hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 1 hour, 36 minutes
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21396197 - 03/12/15 05:57 AM (9 years, 3 months ago) |
|
|
Got me 12 pint sized wheat bran cakes, no vermiculite layer. They are approaching full colonization. Planning on giving this another go with BRF this time in my Eberbach.
Quick question: What is the prescribed BRF to plain water ratio? I mean, how many parts BRF cake do you put to how many parts water and blend?
|
grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
|
Re: Slurry Spawn or Liquid Inoculant [Re: hamloaf]
#21396247 - 03/12/15 06:36 AM (9 years, 3 months ago) |
|
|
2:1 water to PF cake
Going to be blending one up today.
|
hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 1 hour, 36 minutes
|
Re: Slurry Spawn or Liquid Inoculant [Re: grainbrain]
#21396306 - 03/12/15 07:01 AM (9 years, 3 months ago) |
|
|
Thank you. Looking forward to your results. Good luck.
|
tourrat
humanbeinganimal


Registered: 01/16/14
Posts: 394
Last seen: 8 years, 9 months
|
Re: Slurry Spawn or Liquid Inoculant [Re: hamloaf]
#21397246 - 03/12/15 11:46 AM (9 years, 3 months ago) |
|
|
Just spawned 8 wbs jars with a grain slurry. (Cambo on agar to a half pint of wbs used for slurry) was a clumsy mess lol. I see now why you mod the lids ! I didn't really like using the grain slurry ...just felt too clumsy . Water comes out for the first three jars...then trying to jiggle wet grain out.( I need a bigger sab. ) if these jars don't contam I'll be amazed.
I've got another set to do tonight which will be using a brf slurry. I think it will be a little more manageable. After this batch I'm definitely going to mod the lid to a widemouth.
Thanks for all the info!
-------------------- Knee deep in the hotel tub.....
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Slurry Spawn or Liquid Inoculant [Re: grainbrain]
#21399436 - 03/12/15 07:27 PM (9 years, 3 months ago) |
|
|
Quote:
grainbrain said: 2:1 water to PF cake
Going to be blending one up today.
yes and that means 1 pint water to 1/2 pint pf cake. double it up if your using a pint cake jar. your recipe would be 2 pints water to 1 pint cake. but thats enough inoculum for well over 100 jars. once you get the feel for the transfer you can do it lightning quick and have enough for even more jars. it took me a little while to actually get the tek down to make my transfers even. and usually i throw away half the inoculum because i don't need it. what i tend to do, and this is not advised but i do it to see if it works, is if i do have a good volume left over. i may pour a higher volume in the last few jars to see if it colonizes quicker. it usually does. but there is a fine line between over pouring and speed. most of the time i just pour the leftovers in the last jar.
use the brf cakes to inoculate grain spawn. you can use a make shift pour spout to evenly pour the slurry into receiving jars. but i just eye it in. i keep the blender lid on and lift up as i pour. then close the lid after the pour and move to the next jar. now, what im describing has the oster blender jar blade loose and not rtved on. its easier to pour that way. it can be done with the rtved lid but its a little more difficult and i feel the higher up you have to lift the lid your leaving room for error. i pour about a tablespoon per quart jar. it colonizes in 3-4 days with no shake. it allows you to create large volume of spawn very quickly with 1 pf cake. all the grows ive done in the last 4-5 months have been from 1 pf jar. its actually fucking incredible! if i mentioned the yield accomplished from 1 pf cake. or else id use something different. it would make you either cry for joy or anger. what people don't understand is, that most are doing more work than is necessary to get good results. and their arguments are null and void.
Edited by eatyualive (03/12/15 07:30 PM)
|
grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21399481 - 03/12/15 07:37 PM (9 years, 3 months ago) |
|
|
I knocked up 27 quarts today from a single slurried PF cake. I may have overpoured a bit, but after a thorough shake there was no pooling liquid at all. Time will tell.
|
tourrat
humanbeinganimal


Registered: 01/16/14
Posts: 394
Last seen: 8 years, 9 months
|
Re: Slurry Spawn or Liquid Inoculant [Re: grainbrain]
#21399518 - 03/12/15 07:47 PM (9 years, 3 months ago) |
|
|
I'm waiting on the water to cool right now so I can slurry a cake . Thanks for all the tips. This thread has really changed the way I'm looking at cultivation. Really good stuff. The grain I slurried earlier has all pretty much absorbed no pooling to speak of so I'm confident they'll do good.
-------------------- Knee deep in the hotel tub.....
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Slurry Spawn or Liquid Inoculant [Re: tourrat]
#21399539 - 03/12/15 07:52 PM (9 years, 3 months ago) |
|
|
at the end of day 2 it will leap off like crazy.
remember, i use a shmuvbox which uses completely different principles as a SAB. so fine tune the method to cater to your sterile tek.
for me bleach wipes are a must have go to item.
|
tourrat
humanbeinganimal


Registered: 01/16/14
Posts: 394
Last seen: 8 years, 9 months
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21399618 - 03/12/15 08:13 PM (9 years, 3 months ago) |
|
|
Yeah I use a lot of wipes...I gotta build a new taller box...I use a tall 66 q...its just not tall enough for jar to jar stuff...I love it for agar but this is a different beast.I've got several brf pucks so I'm going to be going some muda style pp5 quarts with slurry as well...I've got a ton of projects lol
-------------------- Knee deep in the hotel tub.....
|
hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 1 hour, 36 minutes
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21399904 - 03/12/15 09:23 PM (9 years, 3 months ago) |
|
|
Quote:
eatyualive said:
Quote:
grainbrain said: 2:1 water to PF cake
Going to be blending one up today.
yes and that means 1 pint water to 1/2 pint pf cake. double it up if your using a pint cake jar. your recipe would be 2 pints water to 1 pint cake. but thats enough inoculum for well over 100 jars. once you get the feel for the transfer you can do it lightning quick and have enough for even more jars. it took me a little while to actually get the tek down to make my transfers even. and usually i throw away half the inoculum because i don't need it. what i tend to do, and this is not advised but i do it to see if it works, is if i do have a good volume left over. i may pour a higher volume in the last few jars to see if it colonizes quicker. it usually does. but there is a fine line between over pouring and speed. most of the time i just pour the leftovers in the last jar.
use the brf cakes to inoculate grain spawn. you can use a make shift pour spout to evenly pour the slurry into receiving jars. but i just eye it in. i keep the blender lid on and lift up as i pour. then close the lid after the pour and move to the next jar. now, what im describing has the oster blender jar blade loose and not rtved on. its easier to pour that way. it can be done with the rtved lid but its a little more difficult and i feel the higher up you have to lift the lid your leaving room for error. i pour about a tablespoon per quart jar. it colonizes in 3-4 days with no shake. it allows you to create large volume of spawn very quickly with 1 pf cake. all the grows ive done in the last 4-5 months have been from 1 pf jar. its actually fucking incredible! if i mentioned the yield accomplished from 1 pf cake. or else id use something different. it would make you either cry for joy or anger. what people don't understand is, that most are doing more work than is necessary to get good results. and their arguments are null and void.
Hell, With a simple modification to my Eberbach lid, I'll be using my nifty new LC/I gun to distribute the Wheat Bran Slurry. Nice and even cc distribution made fast and easy, everytime. 
When you say to inoculate grain jars with a brf slurry, are you suggesting/implying that brf slurry is better to take to grains, and grain slurry is better suited for bulk substrate materials in your experience??
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Slurry Spawn or Liquid Inoculant [Re: hamloaf]
#21400023 - 03/12/15 09:50 PM (9 years, 3 months ago) |
|
|
pf cake slurry gets a little chunky. you may want to try it first in small amounts as i think it may get clogged i the tubing on the gun. its not like LI where it slides right through. it is more a mudlike consistency. this is why it catches so fast on grain. the brf/mycelia cling and stick all over the outside of the grain and sort of coat it with mycelia. where an LI may slide down the sides at times. even with a good blend it ends up being a little chunky. that is using brf. i don't know how the wheat bran will blend up as i have no experience with it.
ah, no i just have many brf jars stored that i use for masters doing a bunch of quarts. ill do stem tissues blended in syringes. then inject those into pf jars like an LI but i go directly from stem to media. then once colonized in 3-4 days, blend it up into a slurry and noc 40-100 quarts and have those done in less than a week. ill save a few of those and start the whole process over with grain slurries on the next step. you can see where this is going, it never ends.
I personally think its just another option for me. depending on what i have available or what type of mood im in i would do either because they both worked very well for me. ive had an extremely high success rate using these slurries and the way people grow these days seems to be the opposite ideals on what im doing. i don't really care, but that could be the gap that people are missing on figuring out how to do it properly. but then you have newbs who come in and do it perfectly over and over. i tried it the first time i read it and it worked 100%. it was so damn good, ive been using it ever since. it takes alot of conving to move and change things. it really moved me with the ease and speed of it all. the results are excellent.
i only recently attempted grain slurries. i had used brf slurries to propagate spawn for the last 5 years just messing around with it. i had initially done a few pf jars to bulk subs but i had one of the strongest clones i used that i had grow well for over 5 years. i find now that its too low a spawn ratio. with grain slurries, you have a higher volume of spawn to bulk. so to me, grain slurries are better for spawning to bulk. with 2 quarts wbs grain slurry it colonizes the sub in 3 days compared to using 5-8 quarts of spawn(spawned normally) taking about 3 days maybe a little more. so its less spawn but quicker speed. and yes less yield. im trying to find the absolute minimum spawn i can use per tub with good performance. im still working on that. i plan on trying a 3 quart tub soon.
initially, i thought blending up the grain would trich out immediately when i spawned directly to substrate. muda tried it and it triched on him at fruiting. i attempted the slurries with casing and non casings. both worked with 4 solid flushes and no contamination. ive had 100% success with grain slurries directly to bulk on the number of tubs i tried.
i went past 3 flushes on each without contamination and actually just chunked out the subs to make room for more grows. i still need to try the 3 quart slurry to bulk. thats my next trial. im about to slurry a bunch of ape sometimes soon. ive been extremely lazy and busy.
as far as grain slurries being pure gold. you can actually blend up the grain jar and let it settle. once settled, you can use that cloudy liquid like a GLC. it takes about as long as an LI to colonize so it adds about 3-5 days more time to colonize compared to the grain slurry that has more grain chunks in it when poured. but when i use a lower volume of water to grain its a more mud like consistency. this will take 3 days to colonize. ive been messing with a bunch of different things. i plan on doing some nice tex yellow cap grain slurries soon after my ape slurry fest has commenced. you know its about to get real sick in THE TRIBE. JUST WATCH OUT!
http://www.shroomery.org/forums/showflat.php/Number/21242497#21242497
|
hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 1 hour, 36 minutes
|
Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#21400381 - 03/12/15 11:39 PM (9 years, 3 months ago) |
|
|
Awesome! Everything I wanted to know and more. Gonna try another grain slurry to bulk. This time, I'll mix the slurry into the sub.
Couple of questions; 1. What is the water to grain spawn ratio for a good grain slurry? 2. What is the grain slurry to sub ratio that you use? Thanks.
|
|