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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: blackdust]
#20959202 - 12/10/14 07:43 PM (9 years, 6 months ago) |
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ok here is a peak at what ive been working on. im tring to narrow down a good recipe for grain slurry. thats assuming it even works without triching out. so far 1/2 quart wbs grain to 1 pint of water was far too low of a ratio.
Quote:
eatyualive said: so i slurried 1 quart wbs grain with 1 pint of water to a 66 quart tub with 9 quarts volume substrate cvg. ive tried several ratios and this ones working ok. i think i might try 2 quart wbs grain to 3/4 quart of water slurry next. im guessing that may work out right.
blend looks like wbs soup.
 
day 4. im still counting this one out anyway. im not expecting anything but trich.

Edited by eatyualive (12/10/14 07:54 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: chowster]
#20959294 - 12/10/14 08:02 PM (9 years, 6 months ago) |
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Quote:
chowster said: Wait I don't think muda ground up WBS yet tho. I don't have any cakes right now so I gave it a shot. I noticed that I could see tiny colonization points during recovery. I think it was mycelium. But there were was slight green coloration by day 2. Day 3.5 and the darkish green went boom. The grains that didn't get ground up recovered as well as they could. So my guess is that the opinion that the inside of the grain is a contamination waiting to happen if ground up was correct. So sad... 
for sure. i expected trich. but im hopeful. if this recipe works out. ill try to repeat it several times. ill probably attempt it until i can get working ratio. then attempt it about 5 times each with 3 different clones.
so far ive tried:
1) 1/2 pint wbs grain to 1 pint water slurry: 10 quarts sub cvg 2) 1/2 pint wbs grain to 1 pint water slurry: 10 quarts sub cvg 3) 1 quart wbs grain to 1 pint water slurry: 9 quarts sub cvg 4) next ill try 2 quarts wbs grain to 3/4 quart of water slurry:9 quarts cvg.
Edited by eatyualive (12/10/14 08:03 PM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#20959704 - 12/10/14 09:32 PM (9 years, 6 months ago) |
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My oat slurry bucket triched the fuck out. It colonised well and started to knot. Then it turned green really fast. Kind of what I expected.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: MudaFuka]
#20959715 - 12/10/14 09:35 PM (9 years, 6 months ago) |
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Quote:
MudaFuka said: My oat slurry bucket triched the fuck out. It colonised well and started to knot. Then it turned green really fast. Kind of what I expected.
yeah im expecting that. did you put it in a greenhouse or was it in the bucket?
im thinking the wbs grain is so small that most of it was missed by the blade and kept in tact. i know the oats are larger and probably have more volume of uncolonized inner grain.
Edited by eatyualive (12/10/14 09:36 PM)
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MudaFuka
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Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#20959738 - 12/10/14 09:40 PM (9 years, 6 months ago) |
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Mono bucket. I didn't want a potential trich bomb in my GH.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Slurry Spawn or Liquid Inoculant [Re: MudaFuka]
#20959770 - 12/10/14 09:47 PM (9 years, 6 months ago) |
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Why does everyone favor grains over powder? or am I just crazy? I have worked with both and they carry their own set of cons and pros in my experience.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: blackdust]
#20959781 - 12/10/14 09:49 PM (9 years, 6 months ago) |
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Quote:
blackdust said: Why does everyone favor grains over powder? or am I just crazy? I have worked with both and they carry their own set of cons and pros in my experience.

powder like brf?
i just wanted to see if it would work with grain. muda tried it first but he had trich. another guy posted above who got trich. so that 2 strikes. but im going to attempt a few tubs just for fun. ill give up after 5 tubs of a good ratio trich out.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#20959841 - 12/10/14 10:06 PM (9 years, 6 months ago) |
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Yeah. Or flour out of the WBS, Oats, Rye berries, Rye grass seed, Millet, etc. I don't see to many people going bulk with grain flour.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: blackdust]
#20975330 - 12/14/14 01:03 PM (9 years, 6 months ago) |
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1 QUART WBS GRAIN JAR SLURRY TO 1 PINT WATER
This is an experiment using slurry wbs grain to liquid inoculate bulk substrates. All of this is being tested with a tested Texas Yellow cap clone that has shown decent yield. The jar was blended using the slurry method but with wbs grain instead of a pf cake. I have currently tested 2 recipes. The first was 1/2 quart grain to 1 quart of water spawned to 10 quarts volume substrate. This tub has taken approximately 14 days to colonize and still appears to have 2 more days. The spawn ratio is far too low. What I am searching for is a perfect volume to get colonization in about 3-4 days with about half the spawn ratio I normally use. So my next experiment will be 2 quarts of grain with 3/4th quart of water slurry to 9 Quarts volume of substrate.
Here is an example of the Texas Clone Test. This is a non cased bulk substrate. With 5 quarts of wbs grain to 10 quarts volume sbutstrate.
TESTED TEX CLONE YIELD: TOTAL 237 g 10.35 oz 1st: 168g 6oz 2nd: 69g 2.5oz 3rd: 52g 1.85oz
  Here was the slurry before and after the blend.
 
Im extremely doubtful this will work and I fully expect this tub to contaminate. I will more than likely induce fruiting in a day or two.
TEX YELLOW CAP TUB 4: 1 Quart wbs to 1 pint water slurry/ 66 Quart Sterilites
Substrate Ratio Per Tub: 1:9 Ratio Spawn:substrate
- 1 Quart spawn
- 9 Quarts Volume: 1 Brick Coir/ 1 Quarts Verm/1 cup gypsum
Day by Day: Day 2 to Day 23(dunked for 2nd flush)
     
Edited by eatyualive (12/29/14 03:33 PM)
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azur
God of Fuck



Registered: 04/21/12
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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#20975430 - 12/14/14 01:36 PM (9 years, 6 months ago) |
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Shit. Looks great to me
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Slurry Spawn or Liquid Inoculant [Re: azur]
#20975805 - 12/14/14 03:06 PM (9 years, 6 months ago) |
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so far lol. im expecting green when i induce fruiting. but who knows it might make it. took longer than i wanted to colonize.
Edited by eatyualive (12/14/14 03:08 PM)
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roadrage999
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Registered: 08/22/14
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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#20986083 - 12/16/14 07:03 PM (9 years, 6 months ago) |
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my slurry tub stalled and triched out yesterday. i woke up and popped the top to see that shade of green . My WBS tub has already flushed once and the second flush is pinning now. I think ill stick to the WBS for the time being as the 2 tubs i tried with slurry did not end up with any yield.
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TheMustardTiger


Registered: 08/19/13
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Re: Slurry Spawn or Liquid Inoculant [Re: roadrage999]
#20986114 - 12/16/14 07:10 PM (9 years, 6 months ago) |
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I made some LI from a fully colonized agar plate and it's day two and I see nothing in my oat jars. I wonder if the blade destroyed the myc? I did dump the solution in the center of the jars, but I was under the assumption I would see some recovery at this point.It was my first LI so I'm not too hung up on it. On to the next I guess.
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MudaFuka
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Registered: 12/14/13
Posts: 18,648
Loc: Canada
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It usually takes mine 4 to 5 days to show recovery but then it finishes up pretty quick.
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 10 months
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Re: Slurry Spawn or Liquid Inoculant [Re: MudaFuka]
#20986139 - 12/16/14 07:14 PM (9 years, 6 months ago) |
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Quote:
MudaFuka said: It usually takes mine 4 to 5 days to show recovery but then it finishes up pretty quick.
Good shit. I guess all hope isn't lost. Thanks, Muda.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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did you shake up the quart jar or let it be?
usually after a few days it will begin to show growth in grain. grain is a little slower than substrate or a pf jar.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Slurry Spawn or Liquid Inoculant [Re: roadrage999]
#20990431 - 12/17/14 05:00 PM (9 years, 6 months ago) |
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Quote:
roadrage999 said: my slurry tub stalled and triched out yesterday. i woke up and popped the top to see that shade of green . My WBS tub has already flushed once and the second flush is pinning now. I think ill stick to the WBS for the time being as the 2 tubs i tried with slurry did not end up with any yield.
sorry to hear that. i currently have 4 tubs in fruiting. all experiments at the moment. i have some grain prep going and when its complete i am going to try to find an exact ratio of water to cake/ sub to water so it can be more specific. that is, if the grain slurries don't trich out. i have 3 tubs that are currently on day 14 without a contam.
Edited by eatyualive (12/17/14 05:01 PM)
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roadrage999
Stranger

Registered: 08/22/14
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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
#20995915 - 12/18/14 07:29 PM (9 years, 6 months ago) |
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no worries. It was a good and worthwhile experiment to run. Im not opposed to giving it another shot but instead of a tub i think im going to go with a mini-mono setup and see how it fares in colonizing the smaller area while trying not get get any more of the green.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Slurry Spawn or Liquid Inoculant [Re: roadrage999]
#21008247 - 12/21/14 04:24 PM (9 years, 6 months ago) |
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ok here is an update on my wbs 1 quart slurry. 2 pins began to form today. i see a bunch of knots also. no signs of contamination. you can find images on the log below.
1 QUART WBS GRAIN JAR SLURRY TO 1 PINT WATER
This is an experiment using slurry wbs grain to liquid inoculate bulk substrates. All of this is being tested with a tested Texas Yellow cap clone that has shown decent yield. The jar was blended using the slurry method but with wbs grain instead of a pf cake. I have currently tested 2 recipes. The first was 1/2 quart grain to 1 quart of water spawned to 10 quarts volume substrate. This tub has taken approximately 14 days to colonize and still appears to have 2 more days. The spawn ratio is far too low. What I am searching for is a perfect volume to get colonization in about 3-4 days with about half the spawn ratio I normally use. So my next experiment will be 2 quarts of grain with 3/4th quart of water slurry to 9 Quarts volume of substrate.
Here is an example of the Texas Clone Test. This is a non cased bulk substrate. With 5 quarts of wbs grain to 10 quarts volume sbutstrate.
TESTED TEX CLONE YIELD: TOTAL 237 g 10.35 oz 1st: 168g 6oz 2nd: 69g 2.5oz 3rd: 52g 1.85oz
  Here was the slurry before and after the blend.
 
Im extremely doubtful this will work and I fully expect this tub to contaminate. I will more than likely induce fruiting in a day or two.
TEX YELLOW CAP TUB 4: 1 Quart wbs to 1 pint water slurry/ 66 Quart Sterilites
Substrate Ratio Per Tub: 1:9 Ratio Spawn:substrate
- 1 Quart spawn
- 9 Quarts Volume: 1 Brick Coir/ 1 Quarts Verm/1 cup gypsum
Day by Day: Day 2 to Day 23 (dunked for a 2nd flush)
              
the image below this paragraph is one of the 1 pint wbs grain to water slurry tubs. it began to fruit one fruit in one tub. so i want to show you the failed attempt. although they did not fully colonize they did not contaminate. it is quite funny actually. i hope to get the recipe to get decent yield on the next go around. im going to make some water volume adjustments as well. this substrates moisture content is a little low. so i think that will ruin the yield. but if it will grow ill make the adjustment on the next trial. here is the failed attempt that didnt colonize the sub 100% this has 1 pint wbs grain slurry to 1 pint volume water. this was slurried into 10 quarts cvg.
 pins forming!

Edited by eatyualive (12/29/14 03:41 PM)
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 7 months, 21 days
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Quote:
MustardTiger66 said: I made some LI from a fully colonized agar plate and it's day two and I see nothing in my oat jars. I wonder if the blade destroyed the myc?
I was wondering about other methods of shredding up agar plates, to stop this potential problem of overblending it.
I have an LC with a plastic magnetic stirring turbine vane thing, its from a pump. As its not sharp I hope it is just blending the myc up. As your agar is nice & soft you might have things you could put on the blade to blunt it, like silicone tube or PTFE tape or something. So rather than shredding its breaking it.
For the agar plates I thought about a tool you could use to press down onto it. I was thinking of those things for making french fries. Where is is a grid like structure of sharpened blades.

Something could possibly be made with stanley blades.
 If you have a stack of blades like these they stack together and are about 0.8 or 1mm thick. And they come to a fine point (obviously!) so have a 1mm gap between points. What this means is if you were to stack them and glue or silicone them together you now have a nice chopping tool. You could press it into agar, take it up turn 90 degrees and press it down again. So if they are 1mm thick and you had 51 bonded together it would be 51mm wide with the 2 outer blades 50mm apart and when you press down you have cut 50 strips, and then the second cut makes 2500 squares. And all of them evenly sized.
Now these squares might stick to the blades which could be a problem. If the blades were stainless steel you could he holding them in some clean/sterile manner and just dump the blades into your water jar and shake up to disperse.
After the first press of the blades the agar might rise up and make a mess, so you might just live with the 50 strips, or else run a scalpel down 10 times and then press the blades to get 500 strips.
Another idea would be using metal tubes or plastic tubing or straws or glass tubes or something, maybe even wide syringe needles. You gather a bunch of them up and hold them together with tape or elastic bands or silicone tube tied up.

As it is a round shape you can easily custom make it to fit your petris (if you use round petris). So you can just press it downwards and you have cut up loads of mini agar discs. Again these might become stuck in the tubes so you might need to dump the whole thing into your jar. You could have some matching device to push them out. You will have bits which are not round, the bits in between the holes, but they are evenly sized and should still be of use.
A sterile potato ricer might do the job, the whole idea of them is not to over mash and have consistent size.

Or maybe there is a stainless steel mesh or plastic mesh. A wide sieve might work.
Edited by blackout (12/21/14 05:10 PM)
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