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OfflineProject
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Registered: 06/02/09
Posts: 1,429
Last seen: 12 years, 10 days
Been about 5 days, no signs of growth in LC?
    #10630621 - 07/06/09 02:21 PM (15 years, 6 months ago)

It's been about 5 days or so since I've knocked up my LC.  Did it just like Otto did in his tek.  Used honey as the nutrient source.  I'm almost positive I didn't use an excessive amount of honey.  I was thinking it was a little less that what Otto used.  I used almost 3cc of MS solution (I know, a bit excessive).  I know there are viable spores in my LC because I knocked up some jars with that same syringe and they're doing okay.

Anyway, in my culture, I see the honey sitting around the bottom.  The only things I can see are little puff balls that are floating within the solution.  I know that there is honey that stays semi-clumpy on the bottom of the jar, but it kicks up when I swirl the jar.  Some little fuzz balls stay afloat all the time, but I can't tell if it's little pieces of mycelium or just honey.  I thought the mycelium was supposed to be stringy.

My question is, could these little fuzz balls be mycelium?  If not, how much longer could it take? 

Thanks

Edited by Project (07/06/09 02:22 PM)

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InvisibleDoc_T
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Registered: 03/06/09
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Re: Been about 5 days, no signs of growth in LC? [Re: Project]
    #10630626 - 07/06/09 02:23 PM (15 years, 6 months ago)

Give it another week before you give up.


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OfflineProject
Ride the Spiral


Registered: 06/02/09
Posts: 1,429
Last seen: 12 years, 10 days
Re: Been about 5 days, no signs of growth in LC? [Re: Project]
    #10630637 - 07/06/09 02:26 PM (15 years, 6 months ago)

Okay..  I'm not wanting to give up on it just yet, I'm just a little disheartened that after so many days I don't even have signs of growth yet being that Otto said after a few days I should see the first signs.

My temperature range has been anywhere between 75-79 degrees, but most of the time it's been around 77.

I've been swirling to aerate it every day.

Is it normal to have to wait this long to see growth even if you're within acceptable growth parameters?

Edited by Project (07/06/09 02:27 PM)

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InvisibleFractalXplora
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Re: Been about 5 days, no signs of growth in LC? [Re: Project]
    #10630647 - 07/06/09 02:30 PM (15 years, 6 months ago)

live cultures can be weird sometimes. Normally if it doesn't work try a different honey, then maybe bottled water instead of tap.

I had better results using glucose a.k.a dextrose @ 4%.

but sometimes they just don't work full stop.,!!

hit and miss they are, or maybe its me......and my technique.


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