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OfflineShroomWhore
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Re: 4 quarts of spawn to coir/verm/coffee [Re: 13shrooms]
    #10648051 - 07/09/09 03:12 PM (15 years, 6 months ago)

RR also says layering means you dont have to touch the grain which reduces the risk of breaking open the kernels and releasing bacteria. :wink:

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OfflineABC
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Re: 4 quarts of spawn to coir/verm/coffee [Re: 13shrooms]
    #10648071 - 07/09/09 03:17 PM (15 years, 6 months ago)

Quote:

*If you spawn in layers, the spawn layer recovers very fast which then makes it resistant to contaminants as it
colonizes the layers of manure. BETTER LAYERING BULK




Quote:

*It seems to make sense that mixing would give faster colonization, but my experience is the opposite. By
layering, the mycelium on the grains recovers and knits together, and then rapidly takes off and colonizes the
rest of the substrate. In addition, since mixing 'can' damage the kernels, and a broken kernel is a prime site for
contaminant spores to germinate, layering has the added benefit of less trauma to the spawn medium.
LAYERING VS MIXING CASING




Quote:

*I strongly recommend against leaving any grains on top of a substrate, exposed to air. If the grains dry out and
the mushroom mycelium weakens, they become the perfect place for molds to start. Many growers get away
with it, but the contamination rate will be higher over time. Grains should be covered with at least a very light
layer of substrate, imo. LAYERING CASING




From RR's notes

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Invisible13shroomsM
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Re: 4 quarts of spawn to coir/verm/coffee [Re: ShroomWhore]
    #10648073 - 07/09/09 03:18 PM (15 years, 6 months ago)

Quote:

risk of breaking open the kernels and releasing bacteria. 




:shrug:

if u PC'd your grains and pasteurized your substrate properly no harmful bacteria or other contams should be present unless you introduced them.:shrug:


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OfflinePsuper
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Re: 4 quarts of spawn to coir/verm/coffee [Re: 13shrooms]
    #10648146 - 07/09/09 03:37 PM (15 years, 6 months ago)

Quote:

13shrooms said:
Quote:

risk of breaking open the kernels and releasing bacteria. 




:shrug:

if u PC'd your grains and pasteurized your substrate properly no harmful bacteria or other contams should be present unless you introduced them.:shrug:




From what I understand all mold spores get killed.  However, certain bacteria make endospores which can survive high temperatures.  Here's a link with some information:
http://bacteriology.suite101.com/article.cfm/what_is_a_bacterial_endospore


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: Psuper]
    #10649593 - 07/09/09 08:19 PM (15 years, 6 months ago)

oh RR where art thou


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10649650 - 07/09/09 08:30 PM (15 years, 6 months ago)

ok you fans of layering whats your tek? thick layer of sub at bottom then layer the body, then cover all kernels on top?


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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10649668 - 07/09/09 08:34 PM (15 years, 6 months ago)

I do little layers. Half inch of sub, cover w/ grain, repeat. Finish with a light layer of substrate.
I've got a scoop that's a great size, makes it easy.


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: Doc_T]
    #10649687 - 07/09/09 08:38 PM (15 years, 6 months ago)

ok cool, I think I'll also be crumbling a few pf cakes in the mix to give the spawn ratio a bit of a boost :thumbup:


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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10649709 - 07/09/09 08:42 PM (15 years, 6 months ago)

Good idea- did you dunk them?


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OfflineArcanePerception
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Re: 4 quarts of spawn to coir/verm/coffee [Re: 13shrooms]
    #10650532 - 07/09/09 11:23 PM (15 years, 6 months ago)

Quote:

13shrooms said:
Quote:

risk of breaking open the kernels and releasing bacteria. 




:shrug:

if u PC'd your grains and pasteurized your substrate properly no harmful bacteria or other contams should be present unless you introduced them.:shrug:




I think what he was saying is grains on top make an easy target for introduced contams. Contams will be introduced its not like you can have a sterile spawning tek without a clean room. if a grain on top gets too dry for myc other contams could get a foothold


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: Doc_T]
    #10651618 - 07/10/09 07:04 AM (15 years, 6 months ago)

well I was waiting to dunk some other cakes untill I had my PC and or my stockpot free for the dunk. I guess I wasnt planning on dunking them for the monotub but now that I think about it I dont know why I wouldnt! I have a shitload of cakes ready to dunk check it out... I just have to find a vessel not in use and I will dunk from 10am till about 6pm and prepare the monotub later as well as birth the myriad of other cakes into the fc. do you think 8-10hrs is too short of a dunk?

heres the 5 quarts ill be using, its about half wbs half pf cakes..





I also have all these jars to do something with in addition to the jars to be used for this monotub! I dont know what to do with them I might just dunk and roll em.



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OfflineArcanePerception
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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10651966 - 07/10/09 09:53 AM (15 years, 6 months ago)

5 quarts is perfect for your 56 quart monotub.


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Re: 4 quarts of spawn to coir/verm/coffee [Re: ArcanePerception]
    #10655166 - 07/10/09 10:02 PM (15 years, 6 months ago)

I submerged the cakes at about ten this morning (14 hours ago) I wanna go to sleep and prepare the monotub in the morning, if the cakes are left submerged in the fridge underwater for 24 hours is that too long????


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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10655174 - 07/10/09 10:03 PM (15 years, 6 months ago)

It's great. Dunk, sleep, spawn. :yesnod:


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: Doc_T]
    #10655195 - 07/10/09 10:10 PM (15 years, 6 months ago)

awesome im stoked, the substrate has been sitting in those 2 vessels it cooled in since I prepared it the day before yesterday, one is my pc so its sealed and the other is a stockpot but I put plastic wrap over the top so the moisture wouldnt leak out, shouldnt be any problem with storing that for 2 days since I've prepared it I hope! I just hope I dont loose all this work to contams I am concerned about how to properly prepare the fc as well as the black plastic bag, layering the sub+spawn then trimming the bag without introducing contams... but the thing is going to be open to the air the whole preparation time....


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10657302 - 07/11/09 10:20 AM (15 years, 6 months ago)

Okay so today was D day,

first I drilled holes and put my black plastic bag liner (turned inside out) in the monotub.




next I took the pf cakes i was going to use to boost the spawn ratio and crumbled them in individual plastic baggies




then I preceeded to layer the spawn with the substrate, only ended up with about a pint of sub left so I think I did pretty good!
also inserted polyfill at this time...



NOW WHAT???????????????????



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OfflineLightShedder
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Re: 4 quarts of spawn to coir/verm/coffee [Re: antimatt3r]
    #10657320 - 07/11/09 10:27 AM (15 years, 6 months ago)

Let them colonize for about a week without any FAE.

Then turn on the ceiling fan in the room that the tubs are in.

If this is not possible due to circumstance then just make sure there is steady air exchange in the enclosed area where the tubs are placed.

I would recommend that you don't open the tubs again until they are ready for harvest.


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Re: 4 quarts of spawn to coir/verm/coffee [Re: LightShedder]
    #10660961 - 07/11/09 10:48 PM (15 years, 6 months ago)

I would recommend taping over polyfil holes during colonization. Then, plugging with polyfil when you want to initiate pinning.


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Re: 4 quarts of spawn to coir/verm/coffee [Re: Lucid_Euphoria]
    #10661169 - 07/11/09 11:56 PM (15 years, 6 months ago)

Quote:

Lucid_Euphoria said:
I would recommend taping over polyfil holes during colonization. Then, plugging with polyfil when you want to initiate pinning.


:headbang3:


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Invisibleantimatt3r
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Re: 4 quarts of spawn to coir/verm/coffee [Re: 13shrooms]
    #10662072 - 07/12/09 09:03 AM (15 years, 6 months ago)

yeah??? hmm.... i misted a few layers cuz the sub on top bad been dried out a little bit but by the time i got to the bottom it was plenty moist, im hoping this misting didnt create too humid/wet of a situation, i was thinking if I did do that, maybe leaving the polyfill in there might compensate for this.... as of now you cant even see in the fc due to the moisture on the sides of the bin :wink:


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