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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Success rate without flow hood?
#10624192 - 07/05/09 10:52 AM (15 years, 6 months ago) |
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I'd like to know opinions of people who have tried it. I work in a room that is toroughly sanitized with bleach and alcohol. I take all the precautions, shower with anti bacterial soap, wash my hair, brush teeth, wear gloves, head net, scrubs, work in a glove box, etc.
I'd like to know if it's possible for me to work with agar without a flow hood. I got RR's video and I got very interested in trying it out but can't a afford a flow hood right now. Would I have any success?
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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infinitechaos7
Mycophagist


Registered: 01/25/09
Posts: 168
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Re: Success rate without flow hood? [Re: AlexP]
#10624210 - 07/05/09 11:00 AM (15 years, 6 months ago) |
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Quote:
AlexP said: I'd like to know opinions of people who have tried it. I work in a room that is toroughly sanitized with bleach and alcohol. I take all the precautions, shower with anti bacterial soap, wash my hair, brush teeth, wear gloves, head net, scrubs, work in a glove box, etc.
I'd like to know if it's possible for me to work with agar without a flow hood. I got RR's video and I got very interested in trying it out but can't a afford a flow hood right now. Would I have any success?
If you have a glovebox/still air box then you can definitely do agar without a flow hood. Pour your plates inside the glovebox and let them cool and solidify in there. Of course you will want to do your agar work in the glovebox as well, although I get away without it in certain situations by holding the plate to which I am transferring spores/tissue upside down (the agar half on top) so as to minimize the likelihood of anything falling onto the agar surface. Then it's just a quick lift of the agar-containing half of the dish followed by a swift dab of the tissue-containing scalpel tip or a spore-covered inoculating loop onto the agar surface.
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HiddenIdentity
My real name is John Doe



Registered: 04/03/09
Posts: 679
Loc: Your moms house.
Last seen: 5 years, 4 months
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I have been wondering the same thing, cause I would love to work with agar to isolate certain mycelium. I bought RR dvd too, and he seamed to put a lot of emphases on using a flow hood for agar work. If you are very sanitary (like you pretty much described) It may work.... but Im just a noob trying to offer my 2c.
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Quote:
If you have a glovebox/still air box then you can definitely do agar without a flow hood.
That's what I though! Thanks, I'll do it all inside the glovebox.
Quote:
but Im just a noob trying to offer my 2c.
Thanks for the 2 c. Here in portugal we say that grain by grain the chicken gets full of grain!
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: Success rate without flow hood? [Re: AlexP]
#10624345 - 07/05/09 11:34 AM (15 years, 6 months ago) |
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I used a GB for years successfully before getting my flowhoods they are soooo nice but only if you can afford them.
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Re: Success rate without flow hood? [Re: hyphae]
#10624361 - 07/05/09 11:38 AM (15 years, 6 months ago) |
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Thanks Hyphae! That's what I'll do. Yeah, they are expensive!
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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mister
Nature nut



Registered: 10/04/08
Posts: 2,926
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Re: Success rate without flow hood? [Re: AlexP]
#10624570 - 07/05/09 12:32 PM (15 years, 6 months ago) |
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I built my flowhood for around $250. all you need to buy online is the filter and blower, then your set.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 5 years, 13 days
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Re: Success rate without flow hood? [Re: mister]
#10625062 - 07/05/09 02:18 PM (15 years, 6 months ago) |
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A blower is something i have really wanted to build. Just need some money.
It can be done in a glovebox. Even with a flowhood, youd still need a glovebox for those contaminated plates that you want to save.
Opening a contaminated petri infront of a flowhood is bad news.
Work quickly and smoothley when opening petri dishes. Open it up for the least amount of time you can. The less its open, less chance for contams to get in.
Im sure you will have good success with the procedures do sanitisation and all the protective gear you wear.
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Re: Success rate without flow hood? [Re: veda_sticks]
#10625258 - 07/05/09 03:01 PM (15 years, 6 months ago) |
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Thanks a lot Veda! I'm planning on buying those plastic pre sterelized petris so I can just throw them out if contamed. I just don't want to risk my grow/bedroom. I'm gonna give it a go!
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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Psilosophy
Stranger

Registered: 09/16/08
Posts: 73
Last seen: 4 years, 8 months
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Re: Success rate without flow hood? [Re: AlexP]
#10625275 - 07/05/09 03:06 PM (15 years, 6 months ago) |
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I've done 25+ G2G Xfers in a closet with some lysol, gloves, rubbing alcohol, and a zippo. I think I lost one jar... but I'm pretty sure I sneezed into it.
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: Success rate without flow hood? [Re: veda_sticks]
#10625315 - 07/05/09 03:16 PM (15 years, 6 months ago) |
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Quote:
veda_sticks said: A blower is something i have really wanted to build. Just need some money.
It can be done in a glovebox. Even with a flowhood, youd still need a glovebox for those contaminated plates that you want to save.
Opening a contaminated petri infront of a flowhood is bad news.
Work quickly and smoothley when opening petri dishes. Open it up for the least amount of time you can. The less its open, less chance for contams to get in.
Im sure you will have good success with the procedures do sanitisation and all the protective gear you wear.
Yep any isolation from contaminated plates and any spore work must be done in a still air glovebox!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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AlexP



Registered: 06/04/09
Posts: 2,270
Loc: Europe
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Re: Success rate without flow hood? [Re: hyphae]
#10627377 - 07/05/09 10:41 PM (15 years, 6 months ago) |
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Thanks a lot guys! I'll give it a go!
-------------------- "To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi
"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley
"The Universe is an artistic catharsis." Artifex Infinitum
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