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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Success rate without flow hood?
    #10624192 - 07/05/09 10:52 AM (15 years, 6 months ago)

I'd like to know opinions of people who have tried it. I work in a room that is toroughly sanitized with bleach and alcohol. I take all the precautions, shower with anti bacterial soap, wash my hair, brush teeth, wear gloves, head net, scrubs, work in a glove box, etc.

I'd like to know if it's possible for me to work with agar without a flow hood. I got RR's video and I got very interested in trying it out but can't a afford a flow hood right now. Would I have any success?


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Invisibleinfinitechaos7
Mycophagist
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Registered: 01/25/09
Posts: 168
Re: Success rate without flow hood? [Re: AlexP]
    #10624210 - 07/05/09 11:00 AM (15 years, 6 months ago)

Quote:

AlexP said:
I'd like to know opinions of people who have tried it. I work in a room that is toroughly sanitized with bleach and alcohol. I take all the precautions, shower with anti bacterial soap, wash my hair, brush teeth, wear gloves, head net, scrubs, work in a glove box, etc.

I'd like to know if it's possible for me to work with agar without a flow hood. I got RR's video and I got very interested in trying it out but can't a afford a flow hood right now. Would I have any success?




If you have a glovebox/still air box then you can definitely do agar without a flow hood.  Pour your plates inside the glovebox and let them cool and solidify in there.  Of course you will want to do your agar work in the glovebox as well, although I get away without it in certain situations by holding the plate to which I am transferring spores/tissue upside down (the agar half on top) so as to minimize the likelihood of anything falling onto the agar surface.  Then it's just a quick lift of the agar-containing half of the dish followed by a swift dab of the tissue-containing scalpel tip or a spore-covered inoculating loop onto the agar surface.

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OfflineHiddenIdentity
My real name is John Doe
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Registered: 04/03/09
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Re: Success rate without flow hood? [Re: infinitechaos7]
    #10624225 - 07/05/09 11:04 AM (15 years, 6 months ago)

I have been wondering the same thing, cause I would love to work with agar to isolate certain mycelium. I bought RR dvd too, and he seamed to put a lot of emphases on using a flow hood for agar work.  If you are very sanitary (like you pretty much described)  It may work....  but Im just a noob trying to offer my 2c.

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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Success rate without flow hood? [Re: HiddenIdentity]
    #10624237 - 07/05/09 11:09 AM (15 years, 6 months ago)

Quote:

If you have a glovebox/still air box then you can definitely do agar without a flow hood.




That's what I though! Thanks, I'll do it all inside the glovebox.

Quote:

but Im just a noob trying to offer my 2c.




Thanks for the 2 c. Here in portugal we say that grain by grain the chicken gets full of grain!

                   
                                :flyhigh:


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
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Re: Success rate without flow hood? [Re: AlexP]
    #10624345 - 07/05/09 11:34 AM (15 years, 6 months ago)

I used a GB for years successfully before getting my flowhoods they are soooo nice but only if you can afford them.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Success rate without flow hood? [Re: hyphae]
    #10624361 - 07/05/09 11:38 AM (15 years, 6 months ago)

Thanks Hyphae! That's what I'll do. Yeah, they are expensive!


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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Invisiblemister
Nature nut
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Registered: 10/04/08
Posts: 2,926
Re: Success rate without flow hood? [Re: AlexP]
    #10624570 - 07/05/09 12:32 PM (15 years, 6 months ago)

I built my flowhood for around $250. all you need to buy online is the filter and blower, then your set.

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Offlineveda_sticks
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Re: Success rate without flow hood? [Re: mister]
    #10625062 - 07/05/09 02:18 PM (15 years, 6 months ago)

A blower is something i have really wanted to build. Just need some money.

It can be done in a glovebox. Even with a flowhood, youd still need a glovebox for those contaminated plates that you want to save.

Opening a contaminated petri infront of a flowhood is bad news.

Work quickly and smoothley when opening petri dishes. Open it up for the least amount of time you can. The less its open, less chance for contams to get in.

Im sure you will have good success with the procedures do sanitisation and all the protective gear you wear.


--------------------

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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Success rate without flow hood? [Re: veda_sticks]
    #10625258 - 07/05/09 03:01 PM (15 years, 6 months ago)

Thanks a lot Veda! I'm planning on buying those plastic pre sterelized petris so I can just throw them out if contamed. I just don't want to risk my grow/bedroom. I'm gonna give it a go!


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

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OfflinePsilosophy
Stranger
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Registered: 09/16/08
Posts: 73
Last seen: 4 years, 8 months
Re: Success rate without flow hood? [Re: AlexP]
    #10625275 - 07/05/09 03:06 PM (15 years, 6 months ago)

I've done 25+ G2G Xfers in a closet with some lysol, gloves, rubbing alcohol, and a zippo. I think I lost one jar... but I'm pretty sure I sneezed into it.

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
Trusted Cultivator
Re: Success rate without flow hood? [Re: veda_sticks]
    #10625315 - 07/05/09 03:16 PM (15 years, 6 months ago)

Quote:

veda_sticks said:
A blower is something i have really wanted to build. Just need some money.

It can be done in a glovebox. Even with a flowhood, youd still need a glovebox for those contaminated plates that you want to save.

Opening a contaminated petri infront of a flowhood is bad news.

Work quickly and smoothley when opening petri dishes. Open it up for the least amount of time you can. The less its open, less chance for contams to get in.

Im sure you will have good success with the procedures do sanitisation and all the protective gear you wear.



Yep any isolation from contaminated plates and any spore work must be done in a still air glovebox!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
InvisibleAlexP
 User Gallery


Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: Success rate without flow hood? [Re: hyphae]
    #10627377 - 07/05/09 10:41 PM (15 years, 6 months ago)

Thanks a lot guys! I'll give it a go!


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum

Extras: Filter Print Post Top
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Shop: Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals Bridgetown Botanicals   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Left Coast Kratom Buy Kratom Extract


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