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OfflineFunction
Stranger
Registered: 02/12/07
Posts: 6
Last seen: 15 years, 5 months
Yellowing liquid culture
    #10608765 - 07/02/09 09:21 AM (15 years, 6 months ago)

I tried making a liquid culture with some karo light corn syrup and some spores. I'm not sure what's going on with the jar now.
I pressure cooked it for 15 minutes and let it cool, inoculated it, and two weeks later this is what I have: a yellowed liquid with white specks on the bottom. Is it mycelium? Is it a contam? Is it caramelized corn syrup? I have no idea. I need some help :confused:


Take a closer look at the pictures for more clarity
The black line is a paper clip. The liquid was clear when I first inoculated it.

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Offlinewhitelight7
Sr.
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Registered: 02/17/09
Posts: 1,250
Loc: Washington Flag
Last seen: 7 years, 6 months
Re: Yellowing liquid culture [Re: Function]
    #10610352 - 07/02/09 03:05 PM (15 years, 6 months ago)

I've had LC's turn yellow like that.  To date I have found no significant correlation between it and contamination. 
It's hard to see exactly what the white stuff is ur talking about, but if there is enough of it, go ahead and shoot up some jars or agar to see what you have.

PS I hope you didn't add any of that Jack I see in the background to the LC? :cheers:


--------------------
Staunch supporter of spontaneous generation:cheers:
           

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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Yellowing liquid culture [Re: Function]
    #10624392 - 07/05/09 11:45 AM (15 years, 6 months ago)

yes i have lc's turn yellow all the time and they still work fine. It's due to the carmelization of  the sugars. your lc is very hazey though which is never a good thing.

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