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Offlinetophernotsocool
friend of earth
Male


Registered: 05/10/09
Posts: 48
Loc: Milky Way Galaxy
Last seen: 15 years, 2 months
another failure
    #10620168 - 07/04/09 10:44 AM (15 years, 6 months ago)

again no myc growth in the jars, i opened the jars and its the same as the last 2 times, a mild yet repugnant foot like odor, large wet stains where innoc took place, and the material in the jar has become compacted and sort of caked but NO growth at all. could this be a gas exchange issue?


--------------------
"and take me disappearing, through the smoke rings of my mind,
down the foggy ruins of time, far past the frozen leaves,
the haunted frightened trees, out to the windy beach,
far from the twisted reach, of crazy sorrow.
yes the dance, beneath the diamond sky, with one hand waving free,
silhouetted by the sea, circled by the circus sands,
with all memory and fate, driven deep beneath the waves,
let me forget about today, until tomorrow."
~bob dylan

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Offlinefrock
member
Registered: 02/22/04
Posts: 262
Last seen: 14 years, 3 days
Re: another failure [Re: tophernotsocool]
    #10620176 - 07/04/09 10:46 AM (15 years, 6 months ago)

Sounds like a bacterial contam.  What substrate/grain?  How long did you sterilize for?

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Offlinetophernotsocool
friend of earth
Male


Registered: 05/10/09
Posts: 48
Loc: Milky Way Galaxy
Last seen: 15 years, 2 months
Re: another failure [Re: frock]
    #10620194 - 07/04/09 10:50 AM (15 years, 6 months ago)

brf/verm/tapwater in 1/2 pints, mask taped holes, covered with foil, 10psi cooker for 2 hours, cooled for 6 hours, innoc'ed in a storage tote glove box, left to sit in the dark warm closet for about 3 weeks and nothing but caked up material and foot stench, but no visible contams.


--------------------
"and take me disappearing, through the smoke rings of my mind,
down the foggy ruins of time, far past the frozen leaves,
the haunted frightened trees, out to the windy beach,
far from the twisted reach, of crazy sorrow.
yes the dance, beneath the diamond sky, with one hand waving free,
silhouetted by the sea, circled by the circus sands,
with all memory and fate, driven deep beneath the waves,
let me forget about today, until tomorrow."
~bob dylan

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Invisiblebumpn
jesus juice


Registered: 05/31/09
Posts: 948
Loc: HELL
Re: another failure [Re: frock]
    #10620197 - 07/04/09 10:50 AM (15 years, 6 months ago)

where did u get ur spores at ?walk us thought ur process. so we can help try to figure out the problem.


--------------------
[if u have PB print PM ME]
{-----------------------------------------------------------------------------------------------------------------}

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
Trusted Cultivator
Re: another failure [Re: bumpn]
    #10620211 - 07/04/09 10:53 AM (15 years, 6 months ago)

Also you removed the tape after inoculating correct?


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Invisiblebumpn
jesus juice


Registered: 05/31/09
Posts: 948
Loc: HELL
Re: another failure [Re: bumpn]
    #10620216 - 07/04/09 10:54 AM (15 years, 6 months ago)

try using 15 psi for 90 min
try using spring or distilled water
or just boil for 90 min .
ive had no issues using city water and doing boil for brf. 2 weeks in and im at 50% colonize


--------------------
[if u have PB print PM ME]
{-----------------------------------------------------------------------------------------------------------------}

Edited by bumpn (07/04/09 11:04 AM)

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Offlinetophernotsocool
friend of earth
Male


Registered: 05/10/09
Posts: 48
Loc: Milky Way Galaxy
Last seen: 15 years, 2 months
Re: another failure [Re: bumpn]
    #10620243 - 07/04/09 11:04 AM (15 years, 6 months ago)

okay i will give the entire process in detail
mixed the verm with water [and yes. proper amounts]
mixed brf into the verm/water mix
filled 4 1/2 pint jars loosely
filled top half inch rim with dry verm
put masking tape strips over the 4 holes of each jar
molded sheets of a-foil around the tops
PC'ed in a 10 psi for 2 hours [on high8 for 30 mins then low3 for the rest]
cooled for 6 hours
brought pc into bathroom
transferred jars into plastic storage tote glovebox
removed foil, didnt remove tape cuz the cover was closed over it..
so i innoced through the tape
flame sterilized needle
innoced 1/4 cc into each hole
brought the tote into my room, placed jars into closet with 75-80 degs F heat, waited a few weeks and nothing.

[edit] psilocybe cubes B+ from sporeworks


--------------------
"and take me disappearing, through the smoke rings of my mind,
down the foggy ruins of time, far past the frozen leaves,
the haunted frightened trees, out to the windy beach,
far from the twisted reach, of crazy sorrow.
yes the dance, beneath the diamond sky, with one hand waving free,
silhouetted by the sea, circled by the circus sands,
with all memory and fate, driven deep beneath the waves,
let me forget about today, until tomorrow."
~bob dylan

Edited by tophernotsocool (07/04/09 11:05 AM)

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Invisibleprismism
 User Gallery


Registered: 05/11/09
Posts: 5,570
Re: another failure [Re: bumpn]
    #10620252 - 07/04/09 11:05 AM (15 years, 6 months ago)

dont use distilled water for preparing your substrate.
spring or tap is fine.
dont incubate in a warm dark closet..
but these arent the reasons for your shortcomings..
could be a few other things like bacteria or moisture content.
you just have to keep trying to you get it right.
most people dont mess up the brf pf tek..its just way too easy.
so give it another whirl.


--------------------
ephemeral anomalous

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Invisibleprismism
 User Gallery


Registered: 05/11/09
Posts: 5,570
Re: another failure [Re: tophernotsocool]
    #10620265 - 07/04/09 11:08 AM (15 years, 6 months ago)

Quote:

tophernotsocool said:
okay i will give the entire process in detail
mixed the verm with water [and yes. proper amounts]
mixed brf into the verm/water mix
filled 4 1/2 pint jars loosely
filled top half inch rim with dry verm
put masking tape strips over the 4 holes of each jar
molded sheets of a-foil around the tops
PC'ed in a 10 psi for 2 hours [on high8 for 30 mins then low3 for the rest]
cooled for 6 hours
brought pc into bathroom
transferred jars into plastic storage tote glovebox
removed foil, didnt remove tape cuz the cover was closed over it..
so i innoced through the tape
flame sterilized needle
innoced 1/4 cc into each hole
brought the tote into my room, placed jars into closet with 75-80 degs F heat, waited a few weeks and nothing.

[edit] psilocybe cubes B+ from sporeworks




you may have burnt your substrate..
i cant think of anything else.


--------------------
ephemeral anomalous

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Offlinetophernotsocool
friend of earth
Male


Registered: 05/10/09
Posts: 48
Loc: Milky Way Galaxy
Last seen: 15 years, 2 months
Re: another failure [Re: hyphae]
    #10620273 - 07/04/09 11:09 AM (15 years, 6 months ago)

Quote:

hyphae said:
Also you removed the tape after inoculating correct?




funny you say that because the tape was very damp, and the holes poked slightly closed up a little because of the tape [which is why i thought maybe gas problem]

but it cant be just the wet masking tape because the first attempt was without anything on the holes besides the foil and the same result happend


--------------------
"and take me disappearing, through the smoke rings of my mind,
down the foggy ruins of time, far past the frozen leaves,
the haunted frightened trees, out to the windy beach,
far from the twisted reach, of crazy sorrow.
yes the dance, beneath the diamond sky, with one hand waving free,
silhouetted by the sea, circled by the circus sands,
with all memory and fate, driven deep beneath the waves,
let me forget about today, until tomorrow."
~bob dylan

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Offlinetazz
the spazz
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Registered: 04/21/09
Posts: 162
Loc: The Great White North
Last seen: 13 years, 7 months
Re: another failure [Re: prismism]
    #10620289 - 07/04/09 11:11 AM (15 years, 6 months ago)

Quote:

prismism said:
Quote:

tophernotsocool said:
okay i will give the entire process in detail
mixed the verm with water [and yes. proper amounts]
mixed brf into the verm/water mix
filled 4 1/2 pint jars loosely
filled top half inch rim with dry verm
put masking tape strips over the 4 holes of each jar
molded sheets of a-foil around the tops
PC'ed in a 10 psi for 2 hours [on high8 for 30 mins then low3 for the rest]
cooled for 6 hours
brought pc into bathroom
transferred jars into plastic storage tote glovebox
removed foil, didnt remove tape cuz the cover was closed over it..
so i innoced through the tape
flame sterilized needle
innoced 1/4 cc into each hole
brought the tote into my room, placed jars into closet with 75-80 degs F heat, waited a few weeks and nothing.

[edit] psilocybe cubes B+ from sporeworks




you may have burnt your substrate..
i cant think of anything else.



Ya, it doesn't sound so bad. I'd scrap the masking tape - not required.

Hmm... Did you remember to shake the hell out of the syringe before inoculating? I forgot once and those jars took forever to germinate (months). A couple never did.

Edited by tazz (07/04/09 11:12 AM)

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Offlinekaneabel
getting my feet wet
Male User Gallery

Registered: 01/10/05
Posts: 133
Loc: Central Indiana
Last seen: 15 years, 1 month
Re: another failure [Re: tophernotsocool]
    #10620295 - 07/04/09 11:12 AM (15 years, 6 months ago)

Make sure when you put foil over the jars its on there really good and also you may want to try using a little less water, I had that mistake with mine

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Offlinetazz
the spazz
 User Gallery


Registered: 04/21/09
Posts: 162
Loc: The Great White North
Last seen: 13 years, 7 months
Re: another failure [Re: tophernotsocool]
    #10620304 - 07/04/09 11:14 AM (15 years, 6 months ago)

Quote:

tophernotsocool said:
funny you say that because the tape was very damp, and the holes poked slightly closed up a little because of the tape [which is why i thought maybe gas problem]

but it cant be just the wet masking tape because the first attempt was without anything on the holes besides the foil and the same result happend



They should germinate even if taped up. It's just that growth will stall later without some gas exchange, so the holes must be open. Leave the tape off, or use micropore tape instead.

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
Trusted Cultivator
Re: another failure [Re: tazz]
    #10621002 - 07/04/09 01:22 PM (15 years, 6 months ago)

Quote:

tazz said:
Quote:

tophernotsocool said:
funny you say that because the tape was very damp, and the holes poked slightly closed up a little because of the tape [which is why i thought maybe gas problem]

but it cant be just the wet masking tape because the first attempt was without anything on the holes besides the foil and the same result happend



They should germinate even if taped up. It's just that growth will stall later without some gas exchange, so the holes must be open. Leave the tape off, or use micropore tape instead.



This is true.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineRJ Tubs 202
Male


Registered: 09/20/08
Posts: 6,734
Loc: USA Flag
Last seen: 4 hours, 12 minutes
Re: another failure [Re: tophernotsocool]
    #10621131 - 07/04/09 01:44 PM (15 years, 6 months ago)

My guess is that either your sterlization was not effective or your inoculation technique introduced contamination.

A few ideas include,

If all the jars had the same odor, the jars were not sterile.
(I go 15 psi for 90 minutes. I'm not sure about the effectiveness of 10 psi)
If your sterile technique was sloppy, I'd usually expect to see various types of contams.

A great troubleshooting tip is, don't inoculate one jar in a PC run.
If you run into problems, and that one jar goes down to contams, then the problem is with PCing technique.
If the one jar stays clean, and all others contam, the problem is with the sterile technique or a contaminated syringe.

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Invisibleprismism
 User Gallery


Registered: 05/11/09
Posts: 5,570
Re: another failure [Re: RJ Tubs 202]
    #10621265 - 07/04/09 02:14 PM (15 years, 6 months ago)

Quote:

RJ Tubs 202 said:
A great troubleshooting tip is, don't inoculate one jar in a PC run.
If you run into problems, and that one jar goes down to contams, then the problem is with PCing technique.
If the one jar stays clean, and all others contam, the problem is with the sterile technique or a contaminated syringe.




this is a good tip. :thumbup:


--------------------
ephemeral anomalous

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Shop: North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)


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